本试验以初始体重为（0.042±0.002）g的拟穴青蟹仔蟹为研究对象，以鱼油和大豆油（1：1）为脂肪源，配制脂肪水平分别为1.93%、3.95%、6.35%、8.14%、10.54%、12.30%、14.22%（实测值）的7种等氮等能试验饲料，养殖期为3周，用以研究饲料脂肪水平对拟穴青蟹仔蟹生长性能、体组成及消化酶活性的影响。每100只拟穴青蟹仔蟹为1个重复，每3个重复饲喂1种试验饲料。结果表明：1）饲料脂肪水平对拟穴青蟹仔蟹的增重率和特定生长率有显著影响（P<0.05）。随着饲料脂肪水平的升高，增重率和特定生长率均呈先增后降的趋势，在饲料脂肪水平为8.14%时，增重率和特定生长率达到最大值。二次曲线回归分析确定当增重率达到最大值时，饲料脂肪水平为7.52%。拟穴青蟹仔蟹的成活率在饲料脂肪水平为14.22%的组最低，显著低于其余各组（P<0.05）。2）饲料脂肪水平对全蟹中粗蛋白质和粗脂肪含量有影响显著（P<0.05）。全蟹粗蛋白质含量随着饲料脂肪水平的升高先升后降，在饲料脂肪水平为8.14%的组全蟹粗蛋白质含量达到最高；全蟹粗脂肪含量则随着饲料脂肪水平的升高而持续上升，在饲料脂肪水平为14.22%的组全蟹粗脂肪含量达到最高。饲料脂肪水平对全蟹中水分和粗灰分含量没有显著影响（P>0.05）。3）随着饲料脂肪水平的升高，拟穴青蟹仔蟹蛋白酶、淀粉酶和脂肪酶活性均呈先增后降的趋势，3种酶活性的最大值均出现在饲料脂肪水平为8.14%的组。由此得出，以增重率为评价指标，拟穴青蟹仔蟹饲料中最适脂肪水平为7.52%。
Juvenile Scylla paramamosain with the average body weight of (0.042±0.002) g were fed seven isonitrogenous and isoenergetic experimental diets with different lipid levels for 3 weeks to investigate the effects of dietary lipid level on growth performance, body composition and digestive enzyme activities of juvenile Scylla paramamosain. Using fish oil and soybean oil (1:1) as the lipid source, the lipid level in the seven experimental diets was 1.93%, 3.95%, 6.35%, 8.14%, 10.54%, 12.30% and 14.22% (measured values), respectively. Each experimental diet had three replicates and each replicate had 100 juvenile Scylla paramamosain. The results showed as follows:1) dietary lipid level significantly affected the weight growth ratio (WGR) and specific growth ratio (SGR) of juvenile Scylla paramamosain (P<0.05). With the increase of dietary lipid level, the WGR and SGR of juvenile Scylla paramamosain were firstly increased and then decreased, and when the dietary lipid level was 8.14%, both WGR and SGR produced the maximum value. The quadratic curvilinear regression analysis showed the best dietary lipid level was 7.52% which could acquire the best WGR. The survival rate (SR) in the group with 14.22% dietary lipid level was the lowest, which was significantly lower than that in other groups (P<0.05). 2) Dietary lipid level had significant effects on body crude protein and ether extract contents in whole crab (P<0.05). The crude protein content in whole crab was firstly increased and then decreased, and when the dietary lipid level was 8.14%, it produced the maximum value. The ether extract contents in whole crab with the increase of dietary lipid level continued to increase, when dietary lipid level was 14.22%, it produced the maximum value. Dietary lipid level had no significant effects on the contents of moisture and ash in whole crab (P>0.05). 3) With the increase of dietary lipid level, the activities of protease, lipase and amylase of juvenile Scylla paramamosain were firstly increased and then decreased, and all of them produced the maximum value when the juvenile Scylla paramamosain fed with diet contained 8.14% lipid. In conclusion, taking WGR as the evaluation index, the suitable dietary lipid level for juvenile Scylla paramamosain should be 7.52%.
[1] 檀东飞,吴国欣,林跃鑫,等.锯缘青蟹营养成分分析[J].福建师范大学学报:自然科学版,2000,16(4):79-84.
[2] 陈福才.锯缘青蟹的无公害养殖技术[J].广西科学院学报,2012,28(2):120-121,130.
[3] 韩耀龙.拟穴青蟹健康养殖模式研究[D].硕士学位论文.汕头:汕头大学,2011:7-10.
[4] ZHOU Q C,ZHOU J B,CHI S Y,et al.Effect of dietary lipid level on growth performance,feed utilization and digestive enzyme of juvenile ivory shell,Babylonia areolate[J].Aquaculture,2007,272(1/2/3/4):535-540.
[5] 陈水春.饲料脂肪及不同脂肪源对鱼类的影响[J].现代渔业信息,2008,23(10):7-11.
[6] 韩春燕,郑清梅,黄勋和.饲料脂肪水平对奥尼罗非鱼生长发育、饲料利用及血液指标的影响[J].中国饲料,2013(8):36-39.
[7] 汪留全,胡王,李海洋,等.饲料中脂肪水平对幼蟹生长和饲料利用率的影响[J].上海水产大学学报,2003,12(1):19-23.
[8] 段青源,麦康森,申屠基康,等.不同蛋白质、脂肪水平对三疣梭子蟹生长和卵巢色素沉积的影响[J].中国水产科学,2011,18(4):809-818.
[9] 索帅,李健,李吉涛,等.蛋白质和脂肪水平对脊尾白虾生长、非特异性免疫力及抗病力的影响[J].中国渔业质量与标准,2015,5(4):40-47.
[10] 孙龙生,王恒,魏凯,等.日粮不同脂肪水平对罗氏沼虾生长及体组织组成的影响[J].饲料工业,2012,33(16):28-32.
[11] BLAIR T,CASTELL J,NEIL S,et al.Evaluation of microdiets versus live feeds on growth,survival and fatty acid composition of larval haddock (Melanogrammus aeglefinus)[J].Aquaculture,2003,225(1/2/3/4):451-461.
[12] AOAC.Official methods of analysis association of official analytical chemists[S].16th ed.Arlington,VA:Association of Official Analytical Chemists International,1995.
[13] 李爱杰.水产动物营养与饲料学[M].北京:中国农业出版社,1996:36-46.
[14] 王猛强,郑昌区,金敏,等.粗脂肪水平对2种规格凡纳滨对虾生长性能、饲料利用和非特异性免疫的影响[J].动物营养学报,2014,26(9):2687-2697.
[15] 孙龙生,王恒.油脂添加水平对罗氏沼虾生长性能、血液生化指标及体组织脂肪酸组成的影响[J].重庆水产,2010(3):9-16.
[16] 徐维娜,刘文斌,沈美芳,等.饲料中不同蛋白质和脂肪水平对克氏螯虾(Procambarus clarkii)生长性能、体组成和消化酶活性的影响[J].海洋与湖沼,2011,42(4):521-529.
[17] 斯烈钢,邹李昶,申屠基康,等.饲料添加不同脂肪及蛋白质水平对日本沼虾(Macrobrachium nipponensis)生长性能、体成分及消化酶活力的影响[J].海洋与湖沼,2014,45(2):400-408.
[18] 手岛新一,金泽昭夫.配合饵料中蛋白质、脂肪和碳水化合物含量对对虾幼体生长和成活率的影响[J].水产科学,1986,5(3):48-51.
[19] 黄文瑾,周铭月,牛晶晶,等.食物的摄入和能量的平衡[J].辽宁中医药大学学报,2014,16(10):79-84.
[20] 宋青春,齐遵利.水产动物营养与配合饲料学[M].北京:中国农业大学出版社,2010:57-59.
[21] 杨志刚,阙有清,纪连元,等.以配合饲料替代杂鱼对中华绒螯蟹生长及消化酶活性的影响[J].大连海洋大学学报,2013,28(3):293-297.
[22] GANGADHARA B N,EESHA M C,VARGHESE T J,et al.Effect of varying protein and lipid levels on the growth of rohu,Labeo rohita[J].Asian Fisheries Society,1997,10(2):139-147.
[23] 丁雪燕,何中央,邱晓力,等.三疣梭子蟹不同生长阶段消化酶活性及配合饲料对其影响的研究[J].动物营养学报,2010,22(2):492-497.
[24] 孙金辉,孙敬锋,孙学亮,等.不同蛋白质含量饲料对中华绒螯蟹生长性能和消化酶活性的影响[J].中国饲料,2012(23):25-27.
[25] 汤鸿,李少菁,王桂忠,等.锯缘青蟹幼体消化酶活力[J].厦门大学学报:自然科学版,1995,34(1):88-93.
[26] 唐黎,王吉桥,程骏驰,等.水产动物消化酶的研究[J].饲料工业,2007,28(2):28-31.
[27] 韩光明,王爱民,徐跑,等.饲料中脂肪水平对吉富罗非鱼幼鱼成活率、肌肉成分及消化酶活性的影响[J].上海海洋大学学报,2010,19(4):469-474.
[28] 向枭,周兴华,陈建,等.饲料中脂肪含量对翘嘴红鲌幼鱼消化酶活性的影响[J].北京水产,2008(5):35-38.
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