动物营养学报 >

2017 , Vol. 29 >Issue 3: 1012 - 1020

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.03.035

研究简报 Short communications

太子参茎叶多糖对断奶仔猪肠道免疫功能、肠黏膜形态结构及盲肠内容物菌群的影响

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  • 1. 福建农林大学中西兽医结合与动物保健福建省高校重点实验室, 福州 350002;
    2. 莆田市优利可农牧发展有限公司, 莆田 351111
陈凌锋(1991-),男,福建漳平人,硕士研究生,研究方向为中西兽医结合。E-mail:807803735@qq.com

收稿日期: 2016-08-24

  网络出版日期: 2017-03-09

基金资助

福建省科技厅产学研重大专项（2014N5004）

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Effects of Radix pseudostellariae Stem and Leaf Polysaccharide on Intestinal Immune Function, Intestinal Mucosal Morphology and Cecum Contents Flora of Weaned Piglets

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  • 1. Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2. Putian Youlike Agriculture and Animal Husbandry Developing Co., Ltd., Putian 351111, China

Received date: 2016-08-24

  Online published: 2017-03-09

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摘要

本试验旨在研究饲粮中添加太子参茎叶多糖对断奶仔猪肠道免疫功能、肠黏膜形态结构和盲肠内容物菌群的影响。选取25日龄断奶的“大白×长白”二元杂交仔猪120头，按体重相近原则随机分成4个组，每组3个重复，每个重复10头猪。对照组饲喂基础饲粮，试验组分别在基础饲粮中添加500（0.05%多糖组）、1 000（0.10%多糖组）和1 500 mg/kg（0.15%多糖组）太子参茎叶多糖。试验期30 d。结果表明：与对照组相比，各试验组十二指肠白细胞介素-4（IL-4）含量均显著升高（P<0.05），0.10%多糖组十二指肠干扰素-γ（IFN-γ）含量显著升高（P<0.05），0.10%和0.15%多糖组回肠分泌型免疫球蛋白A（SIgA）含量均显著升高（P<0.05）；0.10%和0.15%多糖组十二指肠和空肠绒毛高度显著或极显著增加（P<0.05或P<0.01），十二指肠绒毛高度/隐窝深度（V/C）值显著增加（P<0.05）；0.15%多糖组回肠绒毛高度显著增加（P<0.05）；各试验组盲肠内容物中的大肠杆菌数量显著降低（P<0.05），乳酸杆菌数量显著增加（P<0.05）。由此可见，饲粮中添加太子参茎叶多糖能够提高断奶仔猪肠道免疫功能，增加小肠绒毛高度和V/C值，调节盲肠内容物菌群结构，维持肠道微生态平衡；其适宜添加量为0.10%。

关键词： 太子参茎叶多糖; 断奶仔猪; 肠道免疫功能; 肠黏膜形态结构; 盲肠内容物菌群

本文引用格式

陈凌锋, 蔡旭滨, 檀新珠, 林国徐, 马玉芳, 黄一帆 . 太子参茎叶多糖对断奶仔猪肠道免疫功能、肠黏膜形态结构及盲肠内容物菌群的影响[J]. 动物营养学报, 2017 , 29(3) : 1012 -1020 . DOI: 10.3969/j.issn.1006-267x.2017.03.035

Abstract

This study was conducted to investigate the effects of dietary Radix pseudostellariae stem and leaf polysaccharide (RpSLp) on intestinal immune function, intestinal mucosal morphology and cecum contents flora of weaned piglets. One hundred and twenty 25-day-old weaned piglets (Large White×Landrace) with similar average body weight were selected and randomly allocated into four groups with three replicates per group and 10 piglets per replicate. Piglets in the control group were fed a basal diet, and the others in the experimental groups were fed the basal diets supplemented with 500 (0.05% polysaccharide group), 1 000 (0.10% polysaccharide group) and 1 500 RpSLp (0.15% polysaccharide group), respectively. The experiment lasted for 30 days. The results showed as follows:compared with the control group, the duodenum interleukin-4 (IL-4) content in experimental groups was significantly increased (P<0.05), the duodenum interferon-γ (IFN-γ) content in 0.10% polysaccharide group was significantly increased (P<0.05), the ileum secretory immunoglobulin A (SIgA) content in 0.10% and 0.15% polysaccharide groups was significantly improved (P<0.05); both duodenum and jejunum villi height in 0.10% and 0.15% polysaccharide groups was significantly increased (P<0.01 or P<0.01); and the villi height to crypt depth (V/C) value was also significantly increased (P<0.05); the ileum villi height in 0.15% polysaccharide group was significantly improved (P<0.05); the number of Escherichia coli in cecum contents in experimental groups was significantly decreased (P<0.05), but the Lactobacillus number was significantly increased (P<0.05). It is concluded that dietary RpSLp can improve intestinal immune function, increase the intestinal villi height and V/C calue, regulate flora structure of cecum contents, and keep the balance for intestinal microecology of weaned piglets. The optimum additive amount of RpSLp is 0.10%.

Key words： Radix pseudostellariae stem and leaf polysaccharide; weaned piglets; intestinal immune function; intestinal mucosal morphology; cecum contents flora

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