茶皂素对奶牛免疫功能的影响

常肖肖, 张议夫, 赵士萍, 蒋琦辉, 王炳, 方洛云, 熊本海, 蒋林树

doi:10.3969/j.issn.1006-267x.2017.03.038

动物营养学报 >

2017 , Vol. 29 >Issue 3: 1039 - 1045

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.03.038

研究简报 Short communications

茶皂素对奶牛免疫功能的影响

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1. 北京农学院动物科学技术学院, 奶牛营养学北京市重点实验室, 北京 102206;
2. 中国农业科学院北京畜牧兽医研究所, 北京 100193

常肖肖(1993-),女,山东泰安人,硕士研究生,研究方向为奶牛营养与免疫。E-mail:c-x-x2@163.com

收稿日期: 2016-09-09

网络出版日期: 2017-03-09

基金资助

“十二五”国家科技支撑项目（25012BAD14B09）；“十三五”国家重大科技专项（2016YFDO70020，2016YFDO700205）；北京市农业局北京市现代农业产业技术体系奶牛创新团队项目

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Effects of Tea Saponin on Immune Function of Dairy Cows

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1. Key Laboratory for Dairy Cow Nutrition, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
2. Institute of Animal Science and Veterinary, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2016-09-09

Online published: 2017-03-09

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摘要

本试验旨在研究茶皂素对荷斯坦奶牛的血清免疫相关细胞因子含量及其mRNA表达量的影响。试验采用4×4拉丁方设计，选取4头瘤胃瘘管奶牛，每头奶牛分别灌注0（对照）、15、30、45 g/d的茶皂素，预试期14 d，正试期21 d，共4期。采用酶联免疫吸附剂测定（ELISA）法试剂盒测定血清中免疫球蛋白（Ig）、白细胞介素（IL）和肿瘤坏死因子-α（TNF-α）的含量，采用实时荧光定量PCR方法测定淋巴细胞IL-1、IL-6、IL-10和TNF-α mRNA表达量。结果显示：1）30 g/d茶皂素组奶牛血清IgM含量显著高于对照组（P<0.05）。2）15 g/d茶皂素组奶牛血清IL-1含量显著高于对照组和45 g/d茶皂素组（P<0.05）。3）与对照组相比，15、30、45 g/d茶皂素组奶牛血清IL-1、IL-6、IL-10、TNF-α mRNA表达量均无显著差异（P>0.05），但45 g/d茶皂素组可提高以上细胞因子mRNA表达量。由此可见，茶皂素可提高奶牛血清中Ig及免疫相关细胞因子含量，并提高细胞因子IL-6 mRNA表达量，表明茶皂素可提高奶牛免疫功能。

关键词： 茶皂素; 免疫; 细胞因子; 奶牛

本文引用格式

常肖肖, 张议夫, 赵士萍, 蒋琦辉, 王炳, 方洛云, 熊本海, 蒋林树 . 茶皂素对奶牛免疫功能的影响[J]. 动物营养学报, 2017 , 29(3) : 1039 -1045 . DOI: 10.3969/j.issn.1006-267x.2017.03.038

Abstract

This experiment was conducted to study the effects of tea saponin on serum immune related cytokines contents and their mRNA expression levels of Holstein cows. Four rumen fistulated cows were used following a 4×4 Latin square design with different infusion concentrations of tea saponin[0 (control), 15, 30, 45 g/d]. The experiment had 4 periods, and each period consisted of 14 d per feeding period and 7 d sampling period. Enzyme-linked immunosorbent assay (ELISA) method kit was used to measure the contents of immunoglobulins, interleukin and tumor necrosis factor alpha (TNF-α) in serum. Quantitative real-time PCR was used to measure the mRNA expression levels of interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-10 (IL-10) and TNF-α. The results showed as follows:1) the serum immunoglobulin M (IgM) content of dairy cow in 30 g/d tea saponin group was significantly higher than that in control group (P<0.05). 2) The serum IL-1 content in 15 g/d tea saponin group was significantly higher than that in control group and 45 g/d tea saponin group (P<0.05). 3) Compared with the control group, the mRNA expression levels of IL-1, IL-10, IL-6 and TNF-α in 15, 30 and 45 g/d tea saponin groups were not significantly different (P>0.05), but the mRNA expression levels of above cytokines in 45 g/d tea saponin group was increased. In conclusion, tea saponin can improve the contents of immunoglobulins and immune related cytokines in serum, as well as the mRNA expression level of cytokine IL-6, which indicate that tea saponin can improve the immune function of dairy cows.

Key words： tea saponin; immunity; cytokines; dairy cows

参考文献

[1] LAI Z F,SHAO Z Q,CHEN Y Z,et al.Effects of sasanquasaponin on ischemia and reperfusion injury in mouse hearts[J].Journal of Pharmacological Sciences,2004,94(3):313-324.

[2] MURAKAMI T,NAKAMURA J,MATSUDA H,et al.Bioactive saponins and glycosides.ⅩⅤ.Saponin constituents with gastroprotective effect from the seeds of tea plant,Camellia sinensis L. var. assamica PIERRE,cultivated in Sri Lanka:structures of assamsaponins A,B,C,D,and E[J].Chemical and Pharmaceutical Bulletin,1999,47(12):1759-1764.

[3] 柳荣祥.几种山茶属植物皂素的化学及活性研究综述[J].茶叶,1997,23(1):22-25,35.

[4] 彭春雨,孟庆翔,任丽萍,等.植物提取物对奶牛产奶性能和血液指标的影响[J].饲料研究,2011(7):8-10.

[5] SAGESAKA M Y,SUGIURA T,MIWA Y,et al.Effect of tea-leaf saponin on blood pressure of spontaneously hypertensive rats[J].Yakugaku Zasshi,1996,116(5):388-395.

[6] 童勇,李玉山.茶皂素耐缺氧及抗氧化作用的实验研究[J].中外健康文摘,2009,6(22):19-21.

[7] 韩志红,刘义庆,庹新兰,等.茶皂素对荷瘤小鼠肿瘤抑制作用研究[J].武汉职工医学院学报,1998,26(1):5-6.

[8] 李静姬,李国江.糖萜素对雏鸡生产性能和免疫器官的影响[J].中国畜牧兽医,2005,32(1):20-22.

[9] 陈玮,周裔彬,宛晓春,等.茶皂素对肉仔鸡生长性能、屠宰性能和肉质的影响[J].粮食与饲料工业,2010(9):49-51.

[10] 冯仰廉,周建民,张晓明,等.我国奶牛饲料产奶净能值测算方法的研究[J].中国畜牧杂志,1987(1):8-11.

[11] WU T Y,WANG C,DING L Y,et al.Arginine relieves the inflammatory response and enhances the casein expression in bovine mammary epithelial cells induced by lipopolysaccharide[J].Mediators of Inflammation,2016,2016:9618795.

[12] 沈维武,徐锐,詹勇,等.水溶性糖萜素对肉鸡生长性能和小肠黏膜免疫功能的影响[J].中国畜牧杂志,2012,48(1):59-62.

[13] 黄鹏,赵珊珊,张学武,等.糖萜素对固始鸡免疫器官发育及T淋巴细胞亚群的影响[J].中国畜牧兽医,2011,38(12):64-67.

[14] 黄鹏,赵珊珊,张学武,等.糖萜素对固始鸡小肠黏膜结构的影响[J].动物营养学报,2011,23(11):2016-2023.

[15] 曾一鸣,周红丽.糖萜素对长沙黄鸡生长性能和免疫力的影响研究[J].当代畜牧,2005(4):19-20.

[16] 陈宝江,赵学军,谷子林,等.糖萜素对绿壳蛋鸡生产性能的影响[J].兽药与饲料添加剂,2001,6(5):8-10.

[17] 巴德年.微生物学[M].北京市:北京医科大学,中国协和医科大学联合出版社,1998:1034.

[18] KISHIMOTO T,HIRANO T,KIKUTANI H,et al.Regulation of growth and differentiation of human B cells[M]//FELDMANN M,LAMB J R,WOODY J N.Human T Cell Clones.Clifton:Humana Press,1985,9:349-359.

[19] 刘显东,毛海峰.白细胞介素-6的生物学功能及与运动的关系探讨[J].宜春学院学报,2009,31(6):166-168.

[20] CARSWELL E A,OLD L J,KASSEL R L,et al.An endotoxin-indncted serum factor that causes necrosis of tumors[J].Proceedings of the National Academy of Sciences of the United States of America,1975,72(9):3666-3670.

[21] 曹江绒.茶皂素的提取、纯化及在洗发液中的应用研究[D].硕士学位论文.西安:陕西科技大学,2014.

[22] 杨强,张石蕊.茶皂素在动物生产中的应用[J].中国饲料,2007(8):8-10.

[23] 赵娟.茶皂素的精制[D].硕士学位论文.无锡:江南大学,2011.

[24] 王雅静.糖萜素、牛至油对育肥后期肉牛作用效果的研究[D].硕士学位论文.重庆:西南大学,2007.

[25] 袁钟宇,张石蕊,贺喜,等.茶籽多糖及茶皂素对肉鸡生长性能和肠道微生物的影响[J].中国畜牧杂志,2010,46(7):28-31.

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