动物营养学报 >

2017 , Vol. 29 >Issue 7: 2374 - 2383

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.07.020

饲料营养 Feed science and technology

饲粮胆碱和裂殖壶菌油联合添加促进二十二碳六烯酸在鸡蛋卵黄中的富集

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  • 1. 东北农业大学动物科学技术学院, 哈尔滨 150030;
    2. 中国农业科学院饲料研究所, 农业部饲料生物技术重点开放实验室, 生物饲料开发国家工程研究中心, 北京 100081
王浩(1986—),男,黑龙江哈尔滨人,博士研究生,从事蛋品质营养调控研究。E-mail:kxkl3001@163.com

收稿日期: 2016-12-29

  网络出版日期: 2017-07-05

基金资助

现代农业产业技术体系（CARS-41-K13）；家禽产业技术体系北京市创新团队（CARS-PSTP）；国家科技支撑计划（2014BAD13B04）

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Dietary Choline and Schizochytrium Oil Enhance Docosahexaenoic Acid Enrichment in Egg Yolk

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  • 1. Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China;
    2. National Engineering Research Center of Biological Feed, Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China

Received date: 2016-12-29

  Online published: 2017-07-05

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摘要

本试验旨在研究饲粮添加胆碱与裂殖壶菌油（Schizochytrium oil，SO）对鸡蛋卵黄脂质及二十二碳六烯酸（DHA）富集的影响。选取26周龄京红蛋鸡288只，分4组（每组6个重复，每重复12只鸡）。采用2×2因子试验设计，饲粮添加胆碱（500和1 000 mg/kg）和SO（0和0.5%）为2个主效应，共配制4种等氮等能饲粮。预试期1周，正试期8周。结果表明：1）试验期内，各组生产性能和蛋品质均无显著差异（P>0.05）。2）各组卵黄中干物质、粗脂肪、胆固醇及甘油三酯含量均无显著差异（P>0.05）。1 000 mg/kg胆碱组卵黄总磷脂含量显著高于500 mg/kg胆碱组（P<0.05）。胆碱和SO对卵黄总磷脂含量有显著交互作用（P＝0.04），1 000 mg/kg胆碱+0.5%SO组卵黄总磷脂含量最高。3）0.5%SO显著提高卵黄n-3多不饱和脂肪酸（PUFA）和DHA含量（P<0.05），显著降低n-6 PUFA含量及n-6 PUFA/n-3 PUFA值（P<0.05）。胆碱和SO对卵黄中n-3 PUFA（P<0.001）、n-6 PUFA（P=0.01）及DHA（P<0.001）含量和n-6 PUFA/n-3 PUFA值（P＝0.01）有显著交互作用，1 000 mg/kg胆碱+0.5%SO组卵黄中n-3 PUFA和DHA含量显著高于其他组（P<0.05），且n-6 PUFA含量及n-6 PUFA/n-3 PUFA值显著低于其他组（P<0.05）。综上，本试验条件下，饲粮中1 000 mg/kg胆碱和0.5%SO联合添加可促进卵黄DHA富集，且对蛋鸡生产性能和蛋品质无显著影响。

关键词： 胆碱; 卵黄; DHA; 脂肪酸; 裂殖壶菌油

本文引用格式

王浩, 王晓翠, 张海军, 齐广海, 王晶, 许丽, 武书庚 . 饲粮胆碱和裂殖壶菌油联合添加促进二十二碳六烯酸在鸡蛋卵黄中的富集[J]. 动物营养学报, 2017 , 29(7) : 2374 -2383 . DOI: 10.3969/j.issn.1006-267x.2017.07.020

Abstract

The aim of this study was to evaluate the effects of dietary choline and Schizochytrium oil (SO) on lipid and docosahexaenoic acid (DHA) enrichment in egg yolk. Two hundred and eighty-eight 26-week-old Jinghong laying hens were randomly divided into 4 groups consisting of 6 replicates each and 12 birds in each replicate. Using a 2×2 factorial experimental design, four isonitrogenous and isocaloric diets were supplemented with 500 mg/kg choline, 1 000 mg/kg choline, 500 mg/kg choline+0.5% SO and 1 000 mg/kg choline+0.5% SO, respectively. The feeding trial lasted for 8 weeks after 1 week adaption. The results showed as follows: 1) performance and egg quality in all groups were not significantly different (P>0.05). 2)The contents of dry matter, crude fat, cholesterol and triglycerides in yolk of different groups were not significantly different (P>0.05). The total phospholipid content in yolk of 1 000 mg/kg choline group was significantly increased compared with 500 mg/kg choline group (P<0.05). The interaction of choline and SO showed significant on the yolk total phospholipid content (P=0.04). The total phospholipid content in yolk of 1 000 mg/kg choline+0.5% SO group was the highest among all groups. 3) Dietary 0.5% SO significantly increased contents of n-3 polyunsaturated fatty acids (PUFA) and DHA, and significantly decreased n-6 PUFA content and the ratio of n-6 PUFA to n-3 PUFA (P<0.05) in yolk. The interactions of choline and SO were significant on contents of n-3 PUFA (P<0.001), n-6 PUFA (P=0.01) and DHA (P<0.001) as well as the ratio of n-6 PUFA to n-3 PUFA (P=0.01) in yolk. The contents of n-3 PUFA and DHA were significantly higher, while n-6 PUFA content and the ratio of n-6 PUFA to n-3 PUFA were significantly lower in yolk of 1 000 mg/kg choline+0.5% SO group than those in the other groups (P<0.05). It is concluded that dietary 1 000 mg/kg choline and 0.5% SO enhance the DHA enrichment in egg yolk and have no adverse effects on performance and egg quality under the experimental condition.

Key words： choline; egg yolk; DHA; fatty acid; Schizochytrium oil

参考文献

[1] SMUTS C M,BOROD E,PEEPLES J M,et al.High-DHA eggs:feasibility as a means to enhance circulating DHA in mother and infant[J].Lipids,2003,38(4):407-414.  

[2] PARK J H,UPADHAYA S D,KIM I H.Effect of dietary marine microalgae (Schizochytrium) powder on egg production,blood lipid profiles,egg quality,and fatty acid composition of egg yolk in layers[J].Asian-Australasian Journal of Animal Sciences,2015,28(3):391-397.  

[3] KOPPENOL A,DELEZIE E,AERTS J,et al.Effect of the ratio of dietary n-3 fatty acids eicosapentaenoic acid and docosahexaenoic acid on broiler breeder performance,egg quality,and yolk fatty acid composition at different breeder ages[J].Poultry Science,2014,93(3):564-573.  

[4] LEMAHIEU C,BRUNEEL C,TERMOTE-VERHALLE R,et al.Dynamics of omega-3 long chain polyunsaturated fatty acid incorporation in egg yolk by autotrophic microalgal supplementation[J].European Journal of Lipid Science and Technology,2015,117(9):1391-1397.  

[5] 陈秀丽,岳宏源,李连彬,等.裂殖壶菌粉对蛋鸡生产性能、蛋品质、血清生化指标和蛋黄二十二碳六烯酸含量的影响[J].动物营养学报,2014,26(3):701-709.

[6] G?ADKOWSKI W,KIE?BOWICZ G,CHOJNACKA A,et al.Fatty acid composition of egg yolk phospholipid fractions following feed supplementation of Lohmann Brown hens with humic-fat preparations[J].Food Chemistry,2011,126(3):1013-1018.  

[7] 马彦庆,陈斌斌,郑妍,等.固定化磷脂酶A1催化制备DHA型磷脂[J].中国粮油学报,2015,30(3):75-79.

[8] 孙长春,武书庚,张海军,等.饲粮添加大豆磷脂对产蛋鸡生产性能和鸡蛋磷脂含量的影响[J].动物营养学报,2010,22(4):1046-1053.

[9] FOLCH J,LEES M,SLOANE STANLEY G H.A simple method for the isolation and purification of total lipides from animal tissues[J].The Journal of Biological Chemistry,1957,226(1):497-509.

[10] PALACIOS L E,WANG T.Egg-yolk lipid fractionation and lecithin characterization[J].Journal of the American Oil Chemists' Society,2005,82(8):571-578.  

[11] ZHAI Q H,DONG X F,TONG J M,et al.Long-term effects of choline on productive performance and egg quality of brown-egg laying hens[J].Poultry Science,2013,92(7):1824-1829.  

[12] LEMAHIEU C,BRUNEEL C,TERMOTE-VERHALLE R,et al.Effect of different microalgal n-3 PUFA supplementation doses on yolk color and n-3 LC-PUFA enrichment in the egg[J].Algal Research,2014,6:119-123.

[13] GUO Z,VIKBJERG A F,XU X B.Enzymatic modification of phospholipids for functional applications and human nutrition[J].Biotechnology Advances,2005,23(3):203-259.  

[14] ZEISEL S H.Dietary choline:biochemistry,physiology,and pharmacology[J].Nutrition,1981,1(1):95-121.  

[15] 李志琼.α-亚麻酸对产蛋鸡脂质代谢及蛋黄胆固醇沉积的影响及其机理[D].博士学位论文.雅安:四川农业大学,2007.

[16] WEISS J F,JOHNSON R M,NABER E C.Effect of some dietary factors and drugs on cholesterol concentration in the egg and plasma of the hen[J].The Journal of Nutrition,1967,91(1):119-128.

[17] FIELD C J,CLANDININ M T.Modulation of adipose tissue fat composition by diet:a review[J].Nutrition Research,1984,4(4):743-755.  

[18] CHOJNACKA A,G?ADKOWSKI W,KIE?BOWICZ G,et al.Enzymatic enrichment of egg-yolk phosphatidylcholine with α-linolenic acid[J].Biotechnology Letters,2009,31(5):705-709.  

[19] HARALDSSON G G,THORARENSEN A.Preparation of phospholipids highly enriched with n-3 polyunsaturated fatty acids by lipase[J].Journal of the American Oil Chemists' Society,1999,76(10):1143-1149.  

[20] ADLERCREUTZ D,WEHTJE E.An enzymatic method for the synthesis of mixed-acid phosphatidylcholine[J].Journal of the American Oil Chemists' Society,2004,81(6):553-557.  

[21] REDDY J R C,VIJEETA T,KARUNA M S L,et al.Lipase-catalyzed preparation of palmitic and stearic acid-rich phosphatidylcholine[J].Journal of the American Oil Chemists' Society,2005,82(10):727-730.  

[22] VIKBJERG A F,PENG L F,MU H L,et al.Continuous production of structured phospholipids in a packed bed reactor with lipase from Thermomyces lanuginosa[J].Journal of the American Oil Chemists' Society,2005,82(4):237-242.  

[23] 陈伟,林映才,张罕星,等.家禽脂肪酸代谢及其在禽蛋中的沉积和营养调控[J].动物营养学报,2012,24(2):204-211.

[24] VANCE D E,RIDGWAY N D.The methylation of phosphatidylethanolamine[J].Progress in Lipid Research,1988,27(1):61-79.  

[25] SCHNEIDER W J.Receptor-mediated mechanisms in ovarian follicle and oocyte development[J].General and Comparative Endocrinology,2009,163(1/2):18-23.

[26] NGUYEN L N,MA D L,SHUI G H,et al.Mfsd2a is a transporter for the essential omega-3 fatty acid docosahexaenoic acid[J].Nature,2014,509(7501):503-506.  

[27] AYDIN R,COOK M E.The effect of dietary conjugated linoleic acid on egg yolk fatty acids and hatchability in Japanese quail[J].Poultry Science,2004,83(12):2016-2022.  
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