发酵中药渣对妊娠母猪繁殖性能、血浆生化参数和抗氧化指标的影响

黎智华, 李华伟, 张婷, 印遇龙, 王德云, 孔祥峰

doi:10.3969/j.issn.1006-267x.2017.07.025

动物营养学报 >

2017 , Vol. 29 >Issue 7: 2416 - 2422

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.07.025

饲料资源开发 Feed Resources Development

发酵中药渣对妊娠母猪繁殖性能、血浆生化参数和抗氧化指标的影响

展开

1. 南京农业大学, 动物医学院, 南京 210095;
2. 中国科学院亚热带农业生态研究所, 湖南省畜禽健康养殖工程技术研究中心, 农业部中南动物营养与饲料科学观测实验站, 长沙 410125;
3. 湖南省植物功能成分利用协同创新中心, 长沙 410128

黎智华(1992—),女,四川仪陇人,硕士研究生,从事中草药饲料添加剂研究。E-mail:zhli92@126.com

收稿日期: 2016-12-20

网络出版日期: 2017-07-05

基金资助

湖南省战略性新兴产业科技攻关项目（2014GK1007）；中央驻湘科研机构技术创新发展专项（2013TF3006）；中国工程院咨询研究项目（2015-XY-41）

收起

Effects of Fermented Chinese Herb Residues on Reproductive Performance, Plasma Biochemical Parameters and Antioxidant Indices of Pregnant Sows

Expand

1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;
2. Hunan Provincial Engineering Research Center of Healthy Livestock, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
3. Hunan Co-Innovation Center of Utilizing Plant Functional Components, Changsha 410128, China

Received date: 2016-12-20

Online published: 2017-07-05

Fold

摘要

本试验旨在研究发酵中药渣对妊娠母猪繁殖性能的影响，并通过测定血浆生化参数和抗氧化指标探讨其作用机制。试验选用3~5胎次、体况和预产期相近的长白×大白二元母猪75头，随机分为3组，每组25头，对照组饲喂基础饲粮，中药渣组和发酵中药渣组分别在基础饲粮中添加1.5 kg/t的中药渣和发酵中药渣。试验周期为整个妊娠期，统计返情、空怀和流产的母猪数，窝产仔数、产活仔数以及仔猪初生窝重和初生个体重。分别于母猪妊娠第45天、第75天和第114天（分娩当天），每组随机选取8头母猪，耳缘静脉采血，分离血浆，测定生化参数和抗氧化指标。结果表明：饲粮添加中药渣或发酵中药渣对母猪的繁殖性能无显著影响（P>0.05）。与对照组相比，妊娠第114天时，发酵中药渣组的血浆总蛋白（TP）含量显著升高（P>0.05），中药渣组和发酵中药渣组的血浆高密度脂蛋白-胆固醇（HDL-C）和低密度脂蛋白-胆固醇（LDL-C）含量显著升高（P<0.05）；妊娠第45天时，中药渣组和发酵中药渣组的血浆过氧化氢酶（CAT）活性显著升高（P<0.05），同时血浆碱性磷酸酶（ALP）活性显著降低（P<0.05）。综上所述，饲粮添加1.5 kg/t发酵中药渣不影响妊娠母猪的繁殖性能，但可在一定程度上改善机体代谢，增强抗氧化功能。

关键词： 发酵中药渣; 妊娠母猪; 繁殖性能; 生化参数; 抗氧化指标

本文引用格式

黎智华, 李华伟, 张婷, 印遇龙, 王德云, 孔祥峰 . 发酵中药渣对妊娠母猪繁殖性能、血浆生化参数和抗氧化指标的影响[J]. 动物营养学报, 2017 , 29(7) : 2416 -2422 . DOI: 10.3969/j.issn.1006-267x.2017.07.025

Abstract

This experiment was conducted to investigate the effects of fermented Chinese herb residues (CHR) on reproductive performance of pregnant sows, and to explore its mechanism by measuring plasma biochemical parameters and antioxidant indices. A total of 75 Landrace×Large White pregnant sows with 3 to 5 parities, similar body condition and expected date of delivery were randomly allocated to 3 groups with 25 sows per group. The animals in the control group were fed a basal diet, while those in CHR group were fed the basal diet supplemented with 1.5 kg/t CHR, and those in fermented CHR group were fed the basal diet supplemented with 1.5 kg/t fermented CHR. The experiment was throughout the gestational period. The sow numbers of estrus returning, empty, and abortion were recorded, as well as the litter size, alive litter size, and birth litter weight and birth individual weight of piglets. Eight sows per group were randomly selected on days 45, 75 and 114 of pregnancy (day of delivery). The blood was sampled via the ear vein and recovered the plasma for analysis of biochemical parameters and antioxidant indices. The results showed that dietary supplemented with CHR or fermented CHR did not significant affect the reproductive performance of sows (P>0.05). Compared with the control group, the content of plasma total protein in the fermented CHR group was significantly increased on day 114 of pregnancy (P<0.05), and the contents of plasma high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) in the CHR and fermented CHR groups were significantly increased on day 114 of pregnancy (P<0.05); on day 45 of pregnancy, the activity of plasma catalase (CAT) in the CHR and fermented CHR groups was significantly increased (P<0.05), while the activity of plasma alkaline phosphatase (ALP) in the CHR and fermented CHR groups was significantly decreased (P<0.05). In conclusion, dietary supplemented with 1.5 kg/t fermented CHR does not affect the reproductive performance of pregnant sows, but can improve the body metabolism and antioxidant function to a certain extent.

Key words： fermented Chinese herb residues; pregnant sows; reproductive performance; biochemical parameters; antioxidant indices

参考文献

[1] 黎智华,祝倩,姬玉娇,等.六种中药渣的营养成分[J].天然研究产物与开发,2017,29(1):91-95.

[2] 马逊风,马宏军,唐占辉,等.中药渣剩余成分分析及利用途径研究[J].东北师大学报:自然科学版,2004,36(2):108-111.

[3] 谭显东,王向东,杨平,等.康宁木霉固态发酵中药渣制备蛋白饲料[J].四川大学学报:工程科学版,2008,40(4):74-79.

[4] WEN Y L,YAN L P,CHEN C S.Effects of fermentation treatment on antioxidant and antimicrobial activities of four common Chinese herbal medicinal residues by Aspergillus oryzae[J].Journal of Food and Drug Analysis,2013,21(2):219-226.

[5] 王珊,桑秀妹,高华君,等.金莲花药渣发酵物对小鼠应激能力的影响[J].河北联合大学学报:医学版,2013,15(6):800-801.

[6] 李华伟,王宗俊,祝倩,等.饲粮添加发酵中药渣对母猪繁殖性能与子代生长性能的影响[J].天然产物研究与开发,2016,28(10):1534-1539.

[7] 罗杰,贺晓玉,黄朝翔,等.五味子药渣发酵菌质对仔猪血液生理指标和免疫功能的影响[J].湖南农业大学学报:自然科学版,2014,40(2):188-191.

[8] 苏家宜,李华伟,黎智华,等.发酵中药渣对断奶仔猪生长性能和肠黏膜形态结构的影响[J].天然产物研究与开发,2016,28(9):1454-1459.

[9] MYATT L,CUI X L.Oxidative stress in the placenta[J].Histochemistry and Cell Biology,2004,122(4):369-382.

[10] SUGINO N,TAKIGUCHI S,UMEKAWA T,et al.Oxidative stress and pregnancy outcome:a workshop report[J].Placenta,2007,28(Suppl.):S48-S50.

[11] 苏家宜,姬玉娇,张婷,等.发酵中药渣对断奶仔猪血浆生化参数和抗氧化指标的影响[J].天然产物研究与开发,2017,29(2):284-289.

[12] MENG S X,CAO J M,FENG Q,et al.Roles of chlorogenic acid on regulating glucose and lipids metabolism:a review[J].Evidence-Based Complementary and Alternative Medicine,2013,2013(4):801457.

[13] 何道同,王兵,陈珺明.人参皂苷药理作用研究进展[J].辽宁中医药大学学报,2012,14(7):118-121.

[14] CHEW Y L,WONG H C.Gypenosides,the cancer buster from Gynostemma pentaphyllum (Thunb.) Makino and the apoptotic pathways:a review[J].Oriental Pharmacy and Experimental Medicine,2016,16(3):153-164.

[15] 王志江,魏国栋,马思缇.地黄多糖的化学和药理作用研究进展[J].中国实验方剂学杂志,2015,21(16):231-235.

[16] 李磊,刘畅,图门巴雅尔,等.中药免疫增效剂及其在畜禽养殖业中的应用[J].畜牧与饲料科学,2015,36(11):15-17,30.

[17] 尹富贵,孔祥峰,刘合军,等.中草药对仔猪生长性能和血清生化参数的影响[J].中国科学院研究生院学报,2007,24(2):201-206.

[18] 李建平,单安山,程宝晶,等.五味子、柠檬酸对断奶仔猪血清生化指标和免疫功能的影响[J].中国畜牧杂志,2009,45(17):25-29.

[19] JI F,WU G,BLANTON J R,Jr,et al.Changes in weight and composition in various tissues of pregnant gilts and their nutritional implications[J].Journal of Animal Science,2005,83(2):366-375.

[20] HERRERA E.Lipid metabolism in pregnancy and its consequences in the fetus and newborn[J].Endocrine,2002,19(1):43-55.

[21] WU G,FANG Y Z,YANG S,et al.Glutathione metabolism and its implications for health[J].The Journal of Nutrition,2004,134(3):489-492.

[22] ZHENG G,QIU Y Y,ZHANG Q F,et al.Chlorogenic acid and caffeine in combination inhibit fat accumulation by regulating hepatic lipid metabolism-related enzymes in mice[J].British Journal of Nutrition,2014,112(6):1034-1040.

[23] LIM S I,CHO C W,CHOI U K,et al.Antioxidant activity and ginsenoside pattern of fermented white ginseng[J].Journal of Ginseng Research,2010,34(3):168-174.

[24] BERCHIERI-RONCHI C B,KIM S W,ZHAO Y,et al.Oxidative stress status of highly prolific sows during gestation and lactation[J].Animal,2011,5(11):1774-1779.

[25] ICHI I,KOJO S.Antioxidants as biomarkers of oxidative stress[M]//ALDINI G,YEUM K J,NIKI E,et al.Biomarkers for antioxidant defense and oxidative damage:principles and practical applications.Singapore:Blackwell Publishing,2010:35-49.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们