动物营养学报 >

2017 , Vol. 29 >Issue 7: 2535 - 2540

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.07.038

研究简报 Short communications

硫酸化银耳多糖和党参多糖对鸡T淋巴细胞增殖及白细胞介素-2mRNA表达水平的影响

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  • 1. 芜湖职业技术学院生物工程学院, 芜湖 241003;
    2. 南京农业大学动物医学院, 南京 210095
朱广双(1975—),男,安徽芜湖人,讲师,硕士,研究方向为中兽医学。E-mail:627990977@qq.com

收稿日期: 2017-03-23

  网络出版日期: 2017-07-05

基金资助

安徽省教育厅自然科学重点项目（KJ2017A564）；安徽省教育厅自然科学重点项目（KJ2015A401）；校级自然科学研究项目“应用复方中草药防治猪场常见呼吸道疾病的研究”（wzyzr201619）；校级“教学质量与教学改革工程”项目（2016）

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Effect of Sulfated Polysaccharidses from Tremella and Condonpsis pilosula on T Lymphocyte Proliferation and mRNA Expression Level of Interleukin-2 of Broilers

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  • 1. College of Biological Engineering, Wuhu Institute of Technology, Wuhu 241003, China;
    2. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2017-03-23

  Online published: 2017-07-05

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摘要

为研究硫酸化银耳多糖（sTPS70c）和硫酸化党参多糖（sCPPS50c）增强免疫作用机理，本试验以未修饰银耳多糖（TPStp）为对照，采用噻唑蓝（MTT）法和实时荧光定量PCR法测定了sTPS70c和sCPPS50c对鸡T淋巴细胞增殖及白细胞介素-2（IL-2）mRNA表达水平的影响。结果表明，多糖单独加入到外周血淋巴细胞时，sTPS70c和sCPPS50c几乎所有浓度均可显著刺激T淋巴细胞的增殖（P<0.05），而TPStp仅在浓度为3.125 μg/mL时显著刺激T淋巴细胞增殖（P<0.05）；多糖与植物血凝素P（PHA-P）同时加入到外周血淋巴细胞时，sCPPS50c在浓度为0.391~1.563 μg/mL时显著刺激T淋巴细胞增殖（P<0.05）；sTPS70c和sCPPS50c在浓度为1.563 μg/mL时可提高T淋巴细胞IL-2 mRNA的表达水平（P<0.05），其中sTPS70c对IL-2 mRNA的促表达作用显著强于TPStp（P<0.05），且在浓度为1.563 μg/mL时的作用最强。结果提示，硫酸化修饰可以提高多糖的淋巴细胞增殖活性及显著增强IL-2 mRNA的表达，且以sTPS70c的作用较强，这与取代度有一定的相关性。

关键词： 硫酸化银耳多糖; 硫酸化党参多糖; 淋巴细胞增殖; 白细胞介素-2

本文引用格式

朱广双, 胡元亮, 曹侃, 王本忠 . 硫酸化银耳多糖和党参多糖对鸡T淋巴细胞增殖及白细胞介素-2mRNA表达水平的影响[J]. 动物营养学报, 2017 , 29(7) : 2535 -2540 . DOI: 10.3969/j.issn.1006-267x.2017.07.038

Abstract

In order to study the mechanism of sulfated polysaccharidses from Tremella (sTPS70c) and Condonpsis pilosula (sCPPS50c) on immunological enhancement activity, the effects of sTPS70c and sCPPS50c on T lymphocyte proliferation and the mRNA expression level of interleukin-2 (IL-2) were determined by methyl thiazolyl tetrazolium (MTT) assay and real-time fluorescent quantitative PCR assay while taking the unmodified polysaccharidses from Tremella (TPStp) as control. The results showed that in single adding into peripheral lymphocyte, sTPS70c and sCPPS50c almost at all concentrations and TPStp only at 3.125 μg/mL could significantly stimulate T lymphocyte proliferation (P<0.05). In simultaneous adding into peripheral lymphocyte with plant haemagglutinin P (PHA-P), sCPPS50c at 0.391 to 1.563 μg/mL significantly stimulated T lymphocyte proliferation (P<0.05). sTPS70c and sCPPS50c also could significantly promote mRNA expression level of IL-2 in T lymphocyte at 1.563 μg/mL (P<0.05), and the promoting effects of sTPS70c were significantly better than TPStp (P<0.05), especially at 1.563 μg/mL, the effect of sTPS70c was the strongest. These results indicate that sulfated modification can enhance the lymphocyte proliferation and mRNA expression of IL-2, and sTPS70c presents more stronger action, which is related to the degree of substitution at a centain extent.

Key words： sulfated Tremella polysaccharide; sulfated Codonopsis pilosula polysaccharide; lymphocyte proliferation; interleukin-2

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