研究简报 Short communications

复合抗菌肽对山羊瘤胃发酵和酶活性的影响

展开
  • 四川农业大学动物医学院, 动物疫病与人类健康重点实验室, 环境公害与动物疾病 四川省高校重点实验室, 成都 611130
高爽(1993—),女,满族,辽宁锦州人,硕士研究生,研究方向为中西兽医与临床。E-mail:18283542011@163.com

收稿日期: 2016-12-07

  网络出版日期: 2017-07-05

基金资助

“长江学者和创新团队发展计划”创新团队项目(IRT0848);四川农业大学双支计划(3571537)

Effects of Compound Antimicrobial Peptides on Ruminal Fermentation and Enzyme Activities of Goats

Expand
  • Key Laboratory of Environmental Hazard and Animal Diseases, Key Laboratory of Animal Disease and Human Health, College of Veterinary, Medicine of Sichuan Agricultural University, Chengdu 611130, China

Received date: 2016-12-07

  Online published: 2017-07-05

摘要

本试验旨在探讨复合抗菌肽对饲喂不同精料饲粮山羊瘤胃发酵和酶活性的影响。选取18只4月龄雄性山羊,随机分3组,每组6只。对照组(Ⅰ组)、高精料组(Ⅱ组)、高精料抗菌肽组(Ⅲ组)分别饲喂300、600和600 g/(只·d)精料,同时Ⅲ组在精料中添加3.0 g/(只·d)复合抗菌肽。试验期为60 d。结果表明:1)与Ⅰ组相比,Ⅱ组瘤胃液乙酸、总挥发性脂肪酸(T-VFA)、甲烷(CH4)、氨态氮(NH3-N)、尿素氮、微生物蛋白(MCP)浓度与(乙酸+丁酸)/丙酸及木聚糖酶、脂肪酶活性极显著或显著增加(P<0.01或P<0.05),羧甲基纤维素酶(CMCase)、β-葡萄糖苷酶和淀粉酶活性极显著或显著降低(P<0.01或P<0.05),丙酸、丁酸浓度及果胶酶、中性蛋白酶活性无显著变化(P>0.05)。2)与Ⅱ组相比,Ⅲ组瘤胃液丙酸、丁酸、NH3-N浓度及CMCase、果胶酶、中性蛋白酶及脂肪酶活性无显著变化(P>0.05),乙酸、T-VFA、CH4、尿素氮浓度与(乙酸+丁酸)/丙酸及木聚糖酶活性极显著或显著降低(P<0.01或P<0.05),MCP浓度及β-葡萄糖苷酶、淀粉酶活性极显著或显著增加(P<0.01或P<0.05)。由此说明,复合抗菌肽可调节山羊瘤胃发酵模式,提高饲料利用率,是理想的饲料添加剂。

本文引用格式

高爽, 邓俊良, 陈芸, 杨颜铱, 刘旗, 陈憧, 任志华, 左之才, 王娅, 崔恒敏 . 复合抗菌肽对山羊瘤胃发酵和酶活性的影响[J]. 动物营养学报, 2017 , 29(7) : 2548 -2555 . DOI: 10.3969/j.issn.1006-267x.2017.07.040

Abstract

This experiment was evaluated to study the effects of compound antimicrobial peptides (C-AMPs) on ruminal fermentation and enzyme activities of goats fed diets with different concentrates. Eighteen 4-month-old male goats were randomly divided into 3 groups and each group had 6 goats. The groups included control group (group Ⅰ), high-concentrate group (group Ⅱ), and high-concentrate antimicrobial peptide group (group Ⅲ), and fed with 300, 600 and 600 g concentrate per head per day, respectively. Moreover, the concentrate of group Ⅲ supplemented with 3.0 g C-AMPs per head per day. The experiment lasted for 60 days. The results showed as follows: relative to the group Ⅰ, the group Ⅱ showed a significant increase in the concentrations of acetate, total volatile fatty acids (T-VFA), methane (CH4), ammoniacal nitrogen (NH3-N), urea nitrogen, microprotein (MCP), the ratio of (acetate+butyrate) to propionate, the activities of xylanase and lipase in rumen fluid (P<0.01 or P<0.05), and showed a significant decrease in the activities of carboxymethyl cellulose (CMCase), β-glucosidase and amylase (P<0.01 or P<0.05), but did not show a significant change in the concentrations of propionate and butyrate, and the activities of pectinase and neutral protease (P>0.05). Relative to the group Ⅱ, the group Ⅲ had no significant change in the concentrations of propionate, butyrate, NH3-N and the activities of CMCase, pectinase, neutral protease and lipase (P>0.05), but the group Ⅲ showed a significant decrease in the concentrations of acetate, T-VFA, CH4, urea nitrogen, the ratio of (acetate+butyrate) to propionate and the activity of xylanase (P<0.01 or P<0.05), and showed a significant increase in the concentration of MCP and the activities of β-glucosidase and amylase (P<0.01 or P<0.05). The results indicate that dietary C-AMPs can modulate ruminal fermentation model and enhance feed conversion rate, and it can be applied as an ideal feed additive.

参考文献

[1] DAI T H,HUANG Y Y,SHARMA S K,et al.Topical antimicrobials for burn wound infections[J].Recent Patents on Anti-Infective Drug Discovery,2010,5(2):124-151.  

[2] LEGUEN E,CHASSEPOT A,DECHER G,et al.Bioactive coatings based on polyelectrolyte multilayer architectures functionalized by embedded proteins,peptides or drugs[J].Biomolecular Engineering,2007,24(1):33-41.  

[3] MARSHALL S H,ARENAS G.Antimicrobial peptides:a natural alternative to chemical antibiotics and a potential for applied biotechnology[J].Electronic Journal of Biotechnology,2003,6(3):271-284.

[4] LI Y M,XIANG Q,ZHANG Q H,et al.Overview on the recent study of antimicrobial peptides:origins,functions,relative mechanisms and application[J].Peptides,2012,37(2):207-215.  

[5] 李冠楠,夏雪娟,隆耀航,等.抗菌肽的研究进展及其应用[J].动物营养学报,2014,26(1):17-25.

[6] OTVOS L,Jr,INSUG O,ROGERS M E,et al.Interaction between heat shock proteins and antimicrobial peptides[J].Biochemistry,2000,39(46):14150-14159.  

[7] WU S D,ZHANG F R,HUANG Z M,et al.Effects of the antimicrobial peptide cecropin AD on performance and intestinal health in weaned piglets challenged with Escherichia coli[J].Peptides,2012,35(2):225-230.  

[8] CHOI S C,INGALE S L,KIM J S,et al.An antimicrobial peptide-A3:effects on growth performance,nutrient retention,intestinal and faecal microflora and intestinal morphology of broilers[J].British Poultry Science,2013,54(6):738-746.  

[9] 徐名能,黄木家,李永新,等.日粮添加抗菌肽制剂对荷斯坦牛乳中体细胞数的影响[J].中国奶牛,2011,16(16):41-43.

[10] LUO C C,YIN D Y,GAO X J,et al.Goat mammary gland expression of Cecropin B to inhibit bacterial pathogens causing mastitis[J].Animal Biotechnology,2013,24(1):66-78.  

[11] LUO C,CAI S Y,JIA L Y,et al.Study on accurate determination of volatile fatty acids in rumen fluid by capillary gas chromatography[C]//Proceedings of the 5th International Conference on Information Engineering for Mechanics and Materials.Amsterdam:Atlantis Press,2015:386-391.

[12] MOSS A R,JOUANY J P,NEWBOLD J,et al.Methane production by ruminants:its contribution to global warming[J].Annales De Zootechnie,2000,49(3):231-253.  

[13] BRODERICK G A,KANG J H.Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J].Journal of Dairy Science,1980,63(1):64-75.  

[14] 冯仰廉.反刍动物营养学[M].北京:科学出版社,2006.

[15] MCDONALD P,EDWARDS R A,GREENHALGH J F D,等.动物营养学[M].王九峰,李同洲,译.北京:中国农业大学出版社,2007.

[16] TAMATE H,MCGILLIARD A D,JACOBSON N L,et al.Effect of various dietaries on the anatomical development of the stomach in the calf[J].Journal of Dairy Science,1962,45(3):408-420.  

[17] 马惠忠.不同精粗比日粮对内蒙古白绒山羊瘤胃发酵和瘤胃微生物区系的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2008.

[18] LASCANO G J,HEINRICHS A J.Rumen fermentation patterns of dairy heifers fed restricted amounts of high,medium,and low concentrate diets and the addition of Saccharomyces cerevisiae[J].Journal of Animal Science,2007,86(Suppl.1):109.

[19] LILA Z A,MOHAMMED N,TAKAHASHI T,et al.Increase of ruminal fiber digestion by cellobiose and a twin strain of Saccharomyces cerevisiae live cells in vitro[J].Animal Science Journal,2006,77(4):407-413.  

[20] 庞德公,杨红建,曹斌斌,等.高精料全混合日粮中产朊假丝酵母添加水平对体外瘤胃发酵特性和纤维降解的影响[J].动物营养学报,2014,26(4):940-946.

[21] SATTER L D,SLYTER A L L.Effect of ammonia concentration on rumen microbial protein production in vitro[J].British Journal of Nutrition,1974,32(2):199-208.  

[22] COTTA M A,RUSSELL J B.Effect of peptides and amino acids on efficiency of rumen bacterial protein synthesis in continuous culture[J].Journal of Dairy Science,1982,65(2):226-234.  

[23] TRIPATHI M K,KARIM S A.Effect of individual and mixed live yeast culture feeding on growth performance,nutrient utilization and microbial crude protein synthesis in lambs[J].Animal Feed Science and Technology,2010,155(2/3/4):163-171.

[24] 孙宏选.不同来源的蛋白质和非结构性碳水化合物对泌乳奶牛瘤胃微生物酶活性的影响[D].硕士学位论文.北京:中国农业科学院,2006.

[25] 黄庆生,王加启.添加不同酵母培养物对瘤胃纤维分解菌群和纤维素酶活的影响[J].畜牧兽医学报,2005,36(2):144-148.

[26] KAMRA D N,CHAUDHARY L C,AGARWAL N,et al.Growth performance,nutrient utilization,rumen fermentation and enzyme activities in calves fed on Saccharomyces cerevisiae supplemented diet[J].Indian Journal of Animal Sciences,2002,72(6):472-475.

[27] 刘清清.日粮精粗比对绵羊消化和瘤胃消化代谢的影响[D].硕士学位论文.晋中:山西农业大学,2014.

[28] 段迎凯,蒋洪文,薛白,等.瘤胃灌注不同来源淀粉对牦牛瘤胃发酵及血清生化指标的影响[J].动物营养学报,2012,24(8):1484-1492.

[29] 李亚学,王佳堃,孙华,等.不同精粗比下添加维生素B12对体外瘤胃发酵和微生物酶活力的影响[J].动物营养学报,2012,24(10):1888-1896.

[30] 何丹林,温刘发,邓春柳,等.蚕抗菌肽AD-酵母制剂对粤黄鸡肠道消化酶和饲料品质的影响[J].中国家禽,2004,26(7):9-10.

[31] 刘翠玲.饲料中添加微生态制剂、抗菌肽及其复合制剂对鲤鱼生长、消化和非特异性免疫相关酶活性的影响[D].硕士学位论文.上海:上海海洋大学,2015.

[32] 郭威,郭晓军,袁洪水,等.产脂肪酶芽孢杆菌对仔鸡生长性能、肠道脂肪酶活力和微生物菌群的影响[J].中国饲料,2016(14):19-21,25.

[33] 范国歌.高效复合益生菌的研制及对仔猪生产性能影响的研究[D].硕士学位论文.郑州:河南农业大学,2011.
文章导航

/