猪营养 Swine Nutrition

母猪补饲发酵芦笋下脚料对母猪和哺乳仔猪血清抗氧化能力、免疫功能和炎性因子含量的影响

展开
  • 1. 江西农业大学, 江西省动物营养重点实验室, 南昌 330045;
    2. 江西省饲料工业办公室, 南昌 330046
赖涛(1993—),男,江西宜春人,硕士研究生,从事动物营养与饲料研究。E-mail:laitao2017@163.com

收稿日期: 2017-02-17

  网络出版日期: 2017-08-02

基金资助

江西省科技支撑计划项目(20142BBF60005)

Effects of Supplementary Feeding of Sows with Fermented Asparagus By-Product on Antioxidant Capacity, Immune Function and Contents of Inflammatory Factors in Serum of Sows and Suckling Piglets

Expand
  • 1. Jiangxi Province Key Lab of Animal Nutrition, Jiangxi Agricultural University, Nanchang 330045, China;
    2. Jiangxi Province Feed Industry Office, Nanchang 330046, China

Received date: 2017-02-17

  Online published: 2017-08-02

摘要

本试验旨在研究给妊娠后期和哺乳期母猪补饲发酵芦笋下脚料对母猪和哺乳仔猪血清抗氧化能力、免疫功能和炎性因子含量的影响。选择15头膘情、胎次和预产期相近的妊娠母猪,随机分成3组,每组5个重复,每个重复1头猪。A(对照)、B和C组母猪每头每天分别补饲0、0.25和0.50 kg发酵芦笋下脚料。试验从母猪妊娠期的第85天开始至产后第21天结束。在母猪分娩和仔猪断奶时采集母猪耳静脉血液,在仔猪10和21日龄时采集仔猪前腔静脉血液,分别测定血清指标。结果表明:1)C组母猪在仔猪断奶时血清超氧化物歧化酶(T-SOD)活性极显著高于对照组(P<0.01),在母猪分娩和仔猪断奶时血清丙二醛(MDA)含量均极显著低于对照组(P<0.01)。B组哺乳仔猪10日龄时血清T-SOD活性显著高于对照组(P<0.05),血清MDA含量显著低于对照组(P<0.05)。2)C组母猪在母猪分娩时血清生长激素(GH)含量显著高于对照组(P<0.05)。3)C组母猪在母猪分娩时血清免疫球蛋白A(IgA)和免疫球蛋白G(IgG)含量显著高于对照组(P<0.05)。4)B和C组母猪血清白细胞介素-6(IL-6)含量在母猪分娩和仔猪断奶时均极显著低于对照组(P<0.01)。C组母猪在仔猪断奶时血清肿瘤坏死因子-α(TNF-α)含量显著低于对照组(P<0.05)。C组哺乳仔猪10日龄时血清TNF-α含量显著低于对照组(P<0.05)。综上,每头母猪每天补饲0.50 kg 发酵芦笋下脚料可增强其抗氧化能力和免疫功能,提高GH含量,减少炎性反应,同时可提高仔猪的抗氧化能力,减少炎性反应的发生。

本文引用格式

赖涛, 文红, 石显亮, 李玲玉, 何余湧, 陆伟 . 母猪补饲发酵芦笋下脚料对母猪和哺乳仔猪血清抗氧化能力、免疫功能和炎性因子含量的影响[J]. 动物营养学报, 2017 , 29(8) : 2681 -2688 . DOI: 10.3969/j.issn.1006-267x.2017.08.010

Abstract

This study was conducted to investigate the effects of supplementary feeding of sows with fermented asparagus by-product on antioxidant capacity, immune function and the contents of inflammatory factors in serum of sows and suckling piglets from late-pregnancy to the end of lactation. Fifteen pregnant sows with similar body condition, parity and parturition time were randomly assigned to three groups with five replicates per group and one sow per replicate. Sows were fed a basal diet supplemented with 0, 0.25 and 0.50 kg fermented asparagus by-product each day in groups A, B and C, respectively. The experiment lasted from day 85 after pregnancy to day 21 after delivery. Blood samples of sows were collected from the auricular vein at the time of sows delivery and piglets weaning, and blood samples of suckling piglets aged at 10 and 21 days were collected from anterior vena cava, and serum indexes were measured, respectively. The results showed as follows: 1) the activity of superoxide dismutase (T-SOD) in serum of sows in group C was significantly higher than that in control group at piglets weaning (P<0.01), and the content of malondialdehyde (MDA) in serum in group C was significantly lower than that in control group at sows delivery and piglets weaning (P<0.01). The activity of T-SOD in serum of suckling piglets aged at 10 days in group B was significantly higher than that in control group (P<0.05), and the content of MDA in serum in group B was significantly lower than that in control group (P<0.05). 2) The content of growth hormone (GH) in serum of sows in group C was significantly higher than that in control group at sows delivery (P<0.05). 3) The contents of immunoglobulin A (IgA) and immunoglobulin G (IgG) in serum of sows in group C were significantly higher than those in control group at sows delivery (P<0.05). 4) The content of interleukin-6 (IL-6) in serum of sows in groups B and C was significantly lower than that in control group at sows delivery and piglets weaning (P<0.01). The content of tumor necrosis factor-α (TNF-α) in serum of sows in group C was significantly lower than that in control group at piglets weaning (P<0.05). The content of TNF-α in serum of suckling piglets aged at 10 days in group C was significantly lower than that in control group (P<0.05). It is concluded that supplementary feeding sows with 0.50 kg fermented asparagus by-product each day can improve antioxidant capacity and immune function, increase GH content and decrease the inflammatory response of sows, meanwhile, increase antioxidant capacity and decrease inflammatory response of piglets.

参考文献

[1] SHAO Y,POOBRASERT O,KENNELLY E J,et al.Steroidal saponins from Asparagus officinalis and their cytotoxic activity[J].Planta Medica,1997,63(3):258-262.  

[2] 高文庚,王红,武爱国.芦笋罐头加工下脚料营养成分分析[J].中国农村小康科技,2007(1):73-75.

[3] 石传林,冯业涛,秦亮.利用芦笋加工下脚料饲喂泌乳牛的效果[J].饲料博览,2001(2):22-23.

[4] 马玉胜.芦笋下脚料饲喂生长育肥猪的试验效果[J].饲料工业,1996(12):37-38.

[5] 练小华,谢益根,罗垂杨,等.对芦笋下脚料微贮效果的影响研究[J].江西畜牧兽医杂志,2016(3):29-31.

[6] 毛春瑕,石显亮,何余湧,等.补饲发酵芦笋下脚料对母猪粪便形态和乳汁质量的影响[J].动物营养学报,2016,28(6):1867-1876.

[7] 李姣,王珂,王瑞坡,等.芦笋多糖提取纯化工艺及其体外抗氧化研究[J].食品科学,2011,32(8):65-69.

[8] 李娟,王凤山.芦笋多糖的研究进展[J].中国生化药物杂志,2009,30(3):215-217.

[9] 董孝元,方冬芬,杨梅,等.纤维素酶辅助提取芦笋黄酮及其抗氧化活性分析[J].食品科学,2014,35(6):17-23.

[10] 刘国强.芦笋黄酮对D-半乳糖衰老小鼠的抗氧化作用研究[D].硕士学位论文.济南:山东师范大学,2008.

[11] 张盼.芦笋笋基营养成分及活性研究[D].硕士学位论文.南昌:南昌大学,2012.

[12] 欧阳菁,龙綮新,杨林,等.猪生长激素研究进展[J].生物技术通讯,2001,12(2):140-143,160.

[13] 张永红,唐芬芬,邵榆岚,等.重组猪生长激素表达研究进展[J].生物技术进展,2014,4(3):165-170.

[14] 张荣庆,韩正康,陈杰,等.大豆黄酮对母猪免疫功能和血清及初乳中GH、PRL、SS水平的影响[J].动物学报,1995,41(2):201-206.

[15] 霍永久,王恬,许若军.乳源性胰岛素对新生仔猪肠道生长和组织形态学的影响[J]. 家畜生态学报, 2006, 27(5):42-45.

[16] 孟腾飞,韩愈杰,王敬,等.猪血清免疫球蛋白IgG的分离纯化及抗大肠杆菌作用[J].饲料工业,2015,36(7):34-37.

[17] 李冬华,袁瑞荣,孙燕,等.中药芦笋的实验和临床研究Ⅰ、芦笋抗肿瘤作用和免疫调节功能的初步实验研究[J].中国临床药理学杂志,1988,4(1):32-39.

[18] GAUTAM M,DIWANAY S,GAIROLA S,et al.Immunoadjuvant potential of Asparagus racemosus aqueous extract in experimental system[J].Journal of Ethnopharmacology,2004,91(2/3):251-255.

[19] 蔡春水,肖平,张毅,等.骨碎补总黄酮对巨噬细胞分泌细胞因子TNF-α、IL-6水平的影响[J].中国矫形外科杂志,2006,14(15):1185-1187.

[20] 杨勤,庄宗杰,吴锦忠,等.芦笋对小鼠细胞免疫机能的影响[J].贵阳医学院学报,1994,19(S1):172-173.

[21] 朱立华.芦笋(Asparagus officinalis Linne)类黄酮抗炎症作用研究[D].硕士学位论文.济南:山东师范大学,2007.
文章导航

/