本试验旨在研究饲粮中添加不同水平的肌醇对獭兔皮肤中β-连环蛋白（β-catenin）和激素敏感脂酶（HSL）表达的影响。选取（40±1）日龄的同期断奶獭兔120只（公母各占1/2），随机分成4组，每组30只。在4组獭兔的饲粮中分别添加0、25、50、75 mg/kg的肌醇，试验期为3个月。在试验的第30天（2月龄）、第60天（3月龄）和第90天（4月龄），分别取腹部、背中部、臀部皮肤，采用免疫组织化学和蛋白质印迹（Western blot）法对β-catenin和HSL的蛋白质表达和定位进行检测。结果表明：β-catenin在毛囊中广泛表达，在毛根鞘细胞和毛乳头均有棕黄色阳性反应细胞。HSL在毛根鞘细胞，尤其是在内根鞘细胞内，呈现非常明显的棕黑色强阳性表达。饲粮中添加50 mg/kg肌醇可以极显著增加2~4月龄獭兔背中部皮肤毛囊中β-catenin和HSL阳性表达细胞的平均灰度值（P<0.01），极显著增加4月龄獭兔背中部、腹部和臀部皮肤毛囊中β-catenin和HSL阳性表达细胞的平均灰度值（P<0.01）。结果提示，肌醇能够通过上调4月龄獭兔毛囊中β-catenin和HSL的表达来促进毛囊的发育，在本试验中，饲粮肌醇水平达到50 mg/kg时效果最佳。

The aim of the present study was to investigate the effects of different inositol levels in diets on the expression of β-catenin and hormone-sensitive triglayceride lipase (HSL) in skin of Rex rabbits. A total of 120 (40±1)-day-old Rex rabbits (half male and half female) were randomly divided into 4 groups with 30 rabbits per group. Rex rabbits in 4 groups were fed diets with different levels of inositol (0, 25, 50 and 75 mg/kg, respectively), and the experiment lasted for 3 months. The skin of abdomen, dorsocentral region, hip were collected on days 30 (2-month-old), 60 (3-month-old), 90 (4-month-old), respectively, and the protein expression and location of β-catenin and HSL were studied by immunohistochemistry and Western blot methods. The results showed that the β-catenin was widely expressed in hair follicle, and the hair root sheath cells and hair papilla all had brown positive reaction cells. HSL was showed a very obvious dark brown strong positive expression in hair root sheath cells, especially in inner root sheath cells. The addition of 50 mg/kg inositol could extremely significantly increase the mean gray value of β-catenin and HSL positive cells in the hair follicles of the dorsocentral region skin of 2- to 4-month-old Rex rabbits (P<0.01), extremely significantly increase the mean gray value of β-catenin and HSL positive cells in the hair follicles of the dorsocentral region, abdomen and hip skin of 4-month-old Rex rabbits (P<0.01). The results suggest that inositol can promote the growth of hair follicle by increasing the expresstion of β-catenin and HSL of 4-month-old Rex rabbits, and the effect is the best when dietary inositol level is 50 mg/kg in this experiment.