饲料营养 Feed science and technology

亚麻籽和鱼油对蛋鸡蛋黄n-3多不饱和脂肪酸含量与肝脏脂肪酸代谢的影响

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  • 1. 浙江省农业科学院畜牧兽医研究所, 杭州 310021;
    2. 浙江艾格生物科技股份有限公司, 长兴 313100
邓波(1972—),男,黑龙江呼兰人,副研究员,硕士,从事动物营养与饲料研究。E-mail:hljdengbo@126.com

收稿日期: 2017-03-31

  网络出版日期: 2017-08-02

基金资助

浙江省重点科技创新团队项目(2011R50025-1)

Effects of Dietary Flaxseed and Fish Oil on n-3 Polyunsaturated Fatty Acid Content in Egg Yolk and Fatty Acid Metabolism in Liver of Hens

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  • 1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    2. Zhejiang Egg Bioscience Co., Inc., Changxing 313100, China

Received date: 2017-03-31

  Online published: 2017-08-02

摘要

本试验旨在研究饲粮中添加亚麻籽和鱼油对蛋鸡蛋黄n-3多不饱和脂肪酸含量、肝脏脂肪酸组成及其合成代谢基因表达的影响。选择28周龄海兰褐壳蛋鸡96只,随机分为4组,每组24只。对照组饲喂基础饲粮,试验组分别在基础饲粮中添加10%亚麻籽、10%亚麻籽+5%鱼油和5%鱼油。饲养21 d后连续收集7 d鸡蛋,然后每组随机选取4只鸡屠宰取样。结果表明,与对照组相比:1)添加亚麻籽和鱼油均显著增加蛋黄中n-3多不饱和脂肪酸含量(P<0.05),其中单独添加鱼油的蛋黄中二十二碳六烯酸(DHA)和二十碳五烯酸含量最高;2)试验组肝脏单不饱和脂肪酸比例均显著降低(P<0.05),肝脏n-3多不饱和脂肪酸比例均显著增加(P<0.05);3)单独添加鱼油显著降低肝脏脂肪酸延长酶1、脂肪酸延长酶2和去饱和酶(脂肪酸去饱和酶1、脂肪酸去饱和酶2和硬脂酰辅酶A去饱和酶1)基因表达水平(P<0.05)。由此可见,蛋鸡饲粮中单独添加亚麻籽或鱼油可以富集蛋黄n-3多不饱和脂肪酸沉积; 添加亚麻籽促进肝脏α-亚麻酸转化生成DHA过程,表现为上调脂肪酸延长酶和去饱和酶基因表达,添加鱼油显示相反结果。

本文引用格式

邓波, 门小明, 朱冬荣, 陶新, 姚云昕, 徐子伟 . 亚麻籽和鱼油对蛋鸡蛋黄n-3多不饱和脂肪酸含量与肝脏脂肪酸代谢的影响[J]. 动物营养学报, 2017 , 29(8) : 2751 -2761 . DOI: 10.3969/j.issn.1006-267x.2017.08.018

Abstract

This experiment was conducted to investigate the effects of dietary flaxseed and fish oil on n-3 polyunsaturated fatty acid content in egg yolk, fatty acid composition and fatty acid biosynthesis gene expression in liver of hens. Ninety-six Hyline Brown hens were divided into 4 groups with 24 hens per group. Hens in the control group were fed a basal diet, and those in trial groups were fed the basal diet+10% flaxseed, the basal diet+10% flaxseed+5% fish oil, and the basal diet+5% fish oil, respectively. Eggs were collected for 7 days after 21 days feeding, and then 4 hens in each group were slaughtered for samples. The results showed as follows, compared with control group, 1) dietary flaxseed and fish oil significantly increased the n-3 polyunsaturated fatty acid contents in yolk (P<0.05), and contents of docosahexaenoic acid (DHA) and timnodonic acid were the highest in yolk affected by dietary fish oil. 2) The liver monounsaturated fatty acid ratios were significantly decreased (P<0.05), and the liver n-3 polyunsaturated fatty acid ratios were significantly increased (P<0.05) in all trial groups. 3) Dietary fish oil significantly decreased gene expressive levels of ELOVL fatty acid elongase 1, ELOVL fatty acid elongase 2 and fatty acid desaturase such as fatty acid desaturase 1, fatty acid desaturase 2 and stearoyl CoA desaturase 1 (P<0.05). In conclusion, dietary flaxseed or fish oil can concentrate n-3 polyunsaturated fatty acid content in yolk of hens. Dietary flaxseed can promote generation process from α-linolenic acid to DHA, exhibiting up-regulating expressive levels of fatty acid elongase and desaturase genes. Dietary fish oil presented contrary results to flaxseed.

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