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维生素E对日本沼虾生长性能、抗氧化性能及抗氨氮胁迫能力的影响

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  • 1. 浙江省水生生物资源养护与开发技术研究重点实验室, 中国水产科学研究院水生动物繁育与营养重点实验室, 湖州师范学院生命科学学院, 湖州 313000;
    2. 大连海洋大学水产与生命科学学院, 大连 116000
孔有琴(1977—),女,浙江长兴人,讲师,博士,主要从事水产动物营养与饲料研究。E-mail:susankuq@zjhu.edu.cn

收稿日期: 2017-02-07

  网络出版日期: 2017-08-02

基金资助

浙江省重大科技专项计划项目(2014C02011);浙江省自然科学基金项目(LY16C190006);国家自然科学基金项目(31402308);湖州市自然科学资金项目(2015YZ06);浙江省重点研发计划项目(2015C03018)

Effects of Dietary Vitamin E on Growth Performance, Antioxidant Ability and Resistance to Ammonia Nitrogen Stress of Macrobrachium nipponense

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  • 1. Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Chinese Academy of Fishery Sciences, College of Life Sciences, Huzhou University, Huzhou 313000, China;
    2. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116000, China

Received date: 2017-02-07

  Online published: 2017-08-02

摘要

本试验旨在研究饲料中维生素E水平对日本沼虾(Macrobrachium nipponense)幼虾生长性能、抗氧化性能和抗氨氮胁迫能力的影响。选择健壮、平均初始体重为(0.119±0.004) g的900只日本沼虾幼虾,随机分为6个组,每组3个重复,每个重复50只。以维生素E乙酯为添加形式,配制6组维生素E实际含量分别为18.31、37.94、66.07、120.25、212.68和388.96 mg/kg的半纯化饲料(分别记为VE1、VE2、VE3、VE4、VE5和VE6组),饲喂8周,随后进行24 h氨氮胁迫试验。结果显示:1)各组日本沼虾成活率(SR)无显著差异(P>0.05);随饲料维生素E水平的增加,日本沼虾增重率(WGR)呈先增加后下降的变化趋势,VE4组最高,且显著高于VE1组(P<0.05);饲料系数(FCR)变化趋势则与WGR相反,VE4组最低,且显著低于VE1组(P<0.05)。2)氨氮胁迫前,各组日本沼虾肝胰腺中丙二醛(MDA)含量随饲料维生素E水平的增加呈先下降后上升的变化趋势,在VE3组达到最低,且显著低于VE1、VE5和VE6组(P<0.05);各组日本沼虾肝胰腺总超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活力和总抗氧化力(T-AOC)随饲料维生素E水平的增加呈先上升后下降的变化趋势。VE1和VE6组日本沼虾硫氧还蛋白(Trx)mRNA表达量较高,硫氧还蛋白还原酶(TrxR)mRNA表达量则较低。VE3组热休克蛋白60(HSP60)mRNA表达量显著低于其他各组(P<0.05),VE1组热休克同源蛋白70(HSC70) mRNA表达量显著低于其他各组(P<0.05),VE1和VE2组热休克蛋白90(HSP90)mRNA表达显著高于其他各组(P<0.05)。3)氨氮胁迫后,各组日本沼虾肝胰腺SOD、CAT、GSH-Px活力,MDA含量,TrxTrxRHSC70 mRNA表达量的变化趋势与胁迫前相似,HSP60HSP90 mRNA表达量变化与胁迫前相反。氨氮胁迫可降低日本沼虾肝胰腺GSH-Px活力、T-AOC及TrxTrxR mRNA表达量,提高MDA含量及VE4、VE5和VE6组SOD活力。由此可见,饲料中添加120.25 mg/kg维生素E对日本沼虾的生长具有积极的促进作用,饲料中添加66.07、120.25和212.68 mg/kg的维生素E可提高日本沼虾抗氧化性能和抗氨氮胁迫能力。

本文引用格式

孔有琴, 丁志丽, 张易祥, 罗娜, 叶金云 . 维生素E对日本沼虾生长性能、抗氧化性能及抗氨氮胁迫能力的影响[J]. 动物营养学报, 2017 , 29(8) : 2893 -2905 . DOI: 10.3969/j.issn.1006-267x.2017.08.034

Abstract

The present experiment was conducted to evaluate the effects of dietary vitamin E level on growth performance, antioxidant ability and resistance to ammonia nitrogen stress of juvenile Macrobrachium nipponense. A total of 900 healthy juvenile Macrobrachium nipponense with the body weight of (0.119±0.004) g were selected and randomly divided into 6 groups with 3 replicates per group and 50 shrimps per replicate. Vitamin E acetate was added to basal semi-purified diet at six levels to make diets with the actual content of 18.31, 37.94, 66.07, 120.25, 212.68 and 388.96 mg /kg vitamin E (denoted by VE1, VE2, VE3, VE4, VE5 and VE6 groups, respectively), and feeding for 8 weeks. After the feeding experiment, the prawns were subjected to ammonia nitrogen stress for 24 h. The results showed as follows: 1) the survival rate of Macrobrachium nipponense in each group had no significant difference (P>0.05). The weight gain rate showed a trend of firstly increased and then decreased with the dietary vitamin E increasing, and prawns in VE4 group gained the highest value and significantly higher than that in VE1 group (P<0.05). The feed conversion rate had the opposite pattern from the weight gain rate, the lowest value were found in VE4 group and significantly lower than that in VE1 group (P<0.05). 2) Before ammonia nitrogen stress, the maleic dialdehyde (MDA) content in hepatopancreas exhibited a trend of firstly decreased and then increased with dietary vitamin E increasing, the lowest value was found in VE3 group and significantly lower than that in VE1, VE5 and VE6 groups (P<0.05), whereas the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and total antioxidant competence (T-AOC) in hepatopancreas exhibited a trend of firstly increased and then decreased with dietary vitamin E increasing. VE1 and VE6 groups gained the higher thioredoxin (Trx) mRNA expression and the lower thioredoxin reductase (TrxR) mRNA expression. The heat shock protein 60 (HSP60) mRNA expression in VE3 group was significantly lower than that in other groups (P<0.05), the heat shock cognate 70 (HSC70) mRNA expression in VE1 group was significantly lower than that in other groups (P<0.05), the heat shock protein 90 (HSP90) mRNA expression in VE1 and VE2 group was significantly higher than that in other groups (P<0.05). 3) After ammonia nitrogen stress, the SOD, CAT and GSH-Px activities, MDA content and the mRNA expressions of Trx, TrxR and HSC70 of Macrobrachium nipponense in each group had the similar pattern with the before stress, but the mRNA expressions of HSP90 and HSP60 exhibited an opposite trend with the before stress. Ammonia nitrogen stress decreased the GSH-Px activities and T-AOC and the mRNA expressions of Trx and TrxR, increased MDA content and SOD activity of VE4, VE5 and VE6 groups. In conclusion, the results demonstrate that 120.25 mg/kg of dietary vitamin E increase the growth of Macrobrachium nipponense, 66.06, 120.25 and 212.68 mg/kg of dietary vitamin E enhance antioxidant activity and alleviated the toxicity of ammonia nitrogen stress of Macrobrachium nipponense.

参考文献

[1] 美国科学院国家研究委员会.鱼类与甲壳类营养需求[M].麦康森,李鹏,赵建民,译.北京:科学出版社,2015:9,215-217

[2] GALLI F,AZZI A,BIRRINGER M,et al.Vitamin E:emerging aspects and new directions[J].Free Radical Biology & Medicine,2016,102:16-36.

[3] HAMRE K.Metabolism,interactions,requirements and functions of vitamin E in fish[J].Aquaculture Nutrition,2011,17(1):98-115.  

[4] LEE M H,SHIAU S Y.Vitamin E requirements of juvenile grass shrimp,Penaeus monodon,and effects on non-specific immune responses[J].Fish & Shellfish Immunology,2004,16(4):475-485.  

[5] LIU Y,WANG WN,WANG AL,et al.Effects of dietary vitamin E supplementation on antioxidant enzyme activities in Litopenaeus vannamei (Boone,1931) exposed to acute salinity changes[J].Aquaculture,2007,265(1/2/3/4):351-358.  

[6] GRIBOFF J,MORALES D,BERTRAND L,et al.Oxidative stress response induced by atrazine in Palaemonetes argentinus:the protective effect of vitamin E[J].Ecotoxicology and Environmental Safety,2014,108:1-8.  

[7] KAN Y,CENGIZ E I,UGURLU P,et al.The protective role of vitamin E on gill and liver tissue histopathology and micronucleus frequencies in peripheral erythrocytes of Oreochromis niloticus exposed to deltamethrin[J].Environmental Toxicology and Pharmacology,2012,34(2):170-179.  

[8] SALEHI I,KARAMIAN R,KOMAKI A,et al.Effects of vitamin E on lead-induced impairments in hippocampal synaptic plasticity[J].Brain Research,2015,1629:270-281.  

[9] LIU B,XU P,XIE J,et al.Effects of emodin and vitamin E on the growth and crowding stress of Wuchang bream (Megalobrama amblycephala)[J].Fish & Shellfish Immunology,2014,40(2):595-602.  

[10] 牛化欣,雷霁霖,常杰,等.维生素E对高脂饲料养殖大菱鲆生长、脂类代谢和抗氧化性能的影响[J].中国水产科学,2014,21(2):291-299.

[11] REN Q,PAN L Q.Digital gene expression analysis in the gills of the swimming crab (Portunus trituberculatus) exposed to elevated ambient ammonia-N[J].Aquaculture,2014,434:108-114.  

[12] YUE F,PAN L Q,XIE P,et al.Immune responses and expression of immune-related genes in swimming crab Portunus trituberculatus exposed to elevated ambient ammonia-N stress[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2010,157(3):246-251.  

[13] ROMANO N,ZENG C S.Subchronic exposure to nitrite,potassium and their combination on survival,growth,total haemocyte count and gill structure of juvenile blue swimmer crabs,Portunus pelagicus[J].Ecotoxicology and Environmental Safety,2009,72(4):1287-1295.  

[14] 洪美玲,陈立侨,顾顺樟,等.氨氮胁迫对中华绒螯蟹免疫指标及肝胰腺组织结构的影响[J].中国水产科学,2007,14(3):412-418.

[15] OLIVA-TELES A.Nutrition and health of aquaculture fish[J].Journal of Fish Diseases,2012,35(2):83-108.  

[16] 黄云,胡毅,文华,等.维生素E对青鱼幼鱼生长、免疫及抗氨氮胁迫能力的影响[J].水生生物学报,2013,37(3):507-514.

[17] 施兆鸿,张艳亮,高权新,等.饲料维生素E水平影响云纹石斑鱼幼鱼对氨氮胁迫的响应[J].动物营养学报,2015,27(5):1596-1604.

[18] 李新正,刘瑞玉,梁象秋.中国动物志(第四十四卷)[M].北京:科学出版社,2007:128-131.

[19] YANG Y,XIE S Q,LEI W,et al.Effect of replacement of fish meal by meat and bone meal and poultry by-product meal in diets on the growth and immune response of Macrobrachium nipponense[J].Fish & Shellfish Immunology,2004,17(2):105-114.  

[20] 农业部渔业渔政管理局.中国渔业统计年鉴2015[M].北京:中国农业出版社,2016:30.

[21] 丁志丽,张易祥,叶金云,等.鱼粉蛋白与发酵酶解豆粕蛋白不同配比对日本沼虾生长及免疫性能的影响[J].动物营养学报,2015,27(1):154-164.

[22] 斯烈钢,邹李昶,申屠基康,等.饲料添加不同脂肪及蛋白质水平对日本沼虾(Macrobrachium nipponensis)生长性能、体成分及消化酶活力的影响[J].海洋与湖沼,2014,45(2):400-408.

[23] KONG Y Q,DING Z L,DU Z Y,et al.Dietary Copper Requirement of juvenile oriental river prawn Macrobrachium nipponense,and its effects on growth,antioxidant activities,and resistance to Aeromonas hydrophila[J].The Israeli Journal of Aquaculture-Bamidgeh,2014,10:100-112.

[24] 孔有琴,陈立侨,丁志丽,等.日本沼虾MT基因克隆、组织差异性表达及与饲料铜、锌含量的相关性[J].水生生物学报,2015,39(6):1126-1133.

[25] AOAC.Official methods of analysis[S].Arlington,VA:Association of Official Analytical Chemists,1995:1141.

[26] WANG A L,WANG W N,WANG Y,et al.Effect of dietary vitamin C supplementation on the oxygen consumption,ammonia-N excretion and Na+/K+ ATPase of Macrobrachium nipponense exposed to ambient ammonia[J].Aquaculture,2003,220(1/2/3/4):833-841.  

[27] HEGAZI M M,ATTIA Z I,ASHOUR O A.Oxidative stress and antioxidant enzymes in liver and white muscle of Nile tilapia juveniles in chronic ammonia exposure[J].Aquatic Toxicology,2010,99(2):118-125.  

[28] ELSTNER E F,HEUPEL A.Inhibition of nitrite formation from hydroxylammoniumchloride:a simple assay for superoxide dismutase[J].Analytical Biochemistry,1976,70(2):616-620.  

[29] AEBI H.Catalase in vitro[J].Methods in Enzymology, 1984,105:121-126.  

[30] SEDLAK J,LINDSAY R H.Estimation of total,protein-bound,and nonprotein sulfhydryl groups in tissue with Ellman's reagent[J].Analytical Biochemistry,1968,25:192-205.  

[31] TANG R,LIU H M,WANG T B,et al.Mechanisms of selenium inhibition of cell apoptosis induced by oxysterols in rat vascular smooth muscle cells[J].Archives of Biochemistry and Biophysics,2005,441(1):16-24.  

[32] BUEGE J A,AUST S D.Microsomal lipid peroxidation[J].Methods in Enzymology,1978,52:302-310.  

[33] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method[J].Methods, 2001,25(4):402-408.  

[34] HE H Q,LAWRENCE A L.Vitamin E requirement of Penaeus vannamei[J].Aquaculture,1993,118(3/4):245-255.

[35] NIU H X,JIA Y D,HU P,et al.Effect of dietary vitamin E on the growth performance and nonspecific immunity in sub-adult turbot (Scophthalmus maximus)[J].Fish & Shellfish Immunology,2014,41(2):501-506.  

[36] DING Z L,CHEN L Q,DU Z Y,et al.A mixture of fish oil and soybean oil as a dietary lipid source prevents precocity and pormotes growth in juvenile Macrobrachium nipponense (De Haan)[J].Aquaculture Research,2014,45(9):1567-1572.  

[37] 虞冰如,沈竑.日本沼虾饲料最适蛋白质、脂肪含量及能量蛋白比的研究[J].水产学报,1990,14(4):321-327.

[38] KIM S K,TAKEUCHI T,YOKOYAMA M,et al.Effect of dietary taurine levels on growth and feeding behavior of juvenile Japanese flounder Paralichthys olivaceus[J].Aquaculture,2005,250(3/4):765-774.

[39] WANG L M,WANG J,BHARADWAJ A S,et al.Effects of dietary copper sources on growth,tissue copper accumulatioin and physiological responses of Japanese sea bass (Lateolabrax japonicus) (Curier,1828) fed semipurified or practical diets[J].Aquaculture Research,2015,46:1619-1627.  

[40] PAN J H,FENG L,JIANG W D,et al.Vitamin E deficiency depressed fish growth,disease resistance,and the immunity and structural integrity of immune organs in grass carp (Ctenopharyngodon idella):referring to NF-κB,TOR and Nrf2 signaling[J].Fish & Shellfish Immunology,2017,60:219-236.  

[41] DING Z K,LI W F,HUANG J H,et al.Dietary alanyl-glutamine and vitamin E supplements could considerably promote the expression of GPx and PPARα genes,antioxidation,feed utilization,growth,and improve composition of juvenile cobia[J].Aquaculture,2017,470:95-102.  

[42] NOZIK-GRAYCK E,SULIMAN H B,PIANTADOSI C A.Extracellular superoxide dismutase[J].The International Journal of Biochemistry & Cell Biology,2005,37(12):2466-2471.  

[43] 李思萌,吴立新,姜志强,等.饲料脂肪源对大菱鲆幼鱼生长性能和肌肉脂肪酸组成的影响[J].动物营养学报,2015,27(5):1421-1430

[44] 李志华,付京花,唐雪莲,等.维生素E在罗非鱼幼鱼饲料中的应用及耐受性研究[J].动物营养学报,2013,25(7):1648-1655.

[45] LU J,HOLMGREN A.The thioredoxin antioxidant system[J].Free Radical Biology and Medicine,2014,66:75-87.  

[46] 郭伟,田琳,曹建彪,等.石英对人胚肺成纤维细胞TrxR/Trx表达的影响[J].实用医学杂志,2012,28(14):2315-2317.

[47] BRANCO V,CANÁRIO J,HOLMGREN A,et al.Inhibition of the thioredoxin system in the brain and liver of zebra-seabreams exposed to waterborne methylmercury[J].Toxicology and Applied Pharmacology,2011,251(2):95-103.  

[48] 张春勇,陈克嶙,黄金昌,等.谷氧还蛋白1和硫氧还蛋白1基因在云南乌金猪不同组织中的表达特点及L-组氨酸对其在氧化应激细胞中表达的影响[J].动物营养学报,2012,24(12):2415-2423.

[49] 赵晓旭,杨佐君,滑静,等.硒化壳聚糖对种公鸡组织硒含量、硒酶活性及其基因表达的影响[J].动物营养学报,2013,25(5):1085-1092.

[50] 安清聪,张春勇,李美荃,等.谷氧还蛋白1和硫氧还蛋白1基因在高黎贡山猪不同组织中表达规律及维生素E对其在氧化应激细胞中表达的影响[J].动物营养学报,2013,25(8):1825-1835.

[51] 袁俊青.褐牙鲆(Paralichthys olivaceus)Trx1,TRP14Prx1基因的克隆、表达和转录水平的免疫刺激反应[D].硕士学位论文.青岛:中国海洋大学,2015:7-8.

[52] RIETJENS I M C M,BOERSMA M G,HAAN L D,et al.The pro-oxidant chemistry of the natural antioxidants vitamin C,vitamin E,carotenoids and flavonoids[J].Environmental Toxicology and Pharmacology,2002,11(3/4):321-333.

[53] PACITTI D,WANG T,MARTIN S A M,et al.Insights into the fish thioredoxin system:expression profile of thioredoxin and thioredoxin reductase in rainbow trout (Oncorhynchus mykiss) during infection and in vitro stimulation[J].Developmental & Comparative Immunology,2014,42(2):261-277.  

[54] BASU N,TODGHAM A E,ACKERMAN P A,et al.Heat shock protein genes and their functional significance in fish[J].Gene,2002,295(2):173-183.  

[55] 施力光,周雄,荀文娟,等.补饲硒和维生素E对高温季节山羊精液品质、抗氧化酶活性及热休克蛋白表达的影响[J].中国畜牧兽医,2016,43(1):101-107.

[56] 贾爱荣,王传娟,张晓华.大菱鲆组成型热休克蛋白70的全长cDNA克隆及表达分析[J].水生生物学报,2010,34(3):547-553.

[57] 明建华,谢骏,徐跑,等.大黄素、维生素C及其配伍对团头鲂抗拥挤胁迫的影响[J].水生生物学报,2011,35(3):400-413.

[58] 芦光宇.氨氮和亚硝酸氮对克氏原螯虾摄食行为和抗氧化功能的影响[D].硕士学位论文.扬州:扬州大学,2012:15-16.

[59] HONG M L,CHEN L Q,SUN X J,et al.Metabolic and immune responses in Chinese mitten-handed crab (Eriocheir sinensis) juveniles exposed to elevated ambient ammonia[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 2007,145(3):363-369.  

[60] STEBBING A R D.Hormesis-the stimulation of growth by low levels of inhibitors[J].Science of the Total Environment,1982,22(3):213-234.

[61] 王芸.pH、氨氮胁迫对中国对虾细胞凋亡和抗氧化系统影响机理的研究[D].博士学位论文.上海:上海海洋大学,2011:101-122.

[62] XIE Y J,SONG L,WENG Z H,et al.HSP90,HSP60 and sHSP families of heat shock protein genes in channel catfish and their expression after bacterial infections[J].Fish & Shellfish Immunology,2015,44(2):642-651.  
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