研究简报 Short communications

抗生素对仔猪肠道微生物的影响

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  • 1. 扬州大学动物科学与技术学院, 扬州 225000;
    2. 太仓市金诸农业发展有限公司, 太仓 215400;
    3. 江苏徐淮地区淮阴农业科学研究所, 淮安 223001
刘颖(1990—),女,江苏淮安人,博士研究生,研究方向为动物遗传育种与繁殖。E-mail:yddkly@163.com

收稿日期: 2017-02-10

  网络出版日期: 2017-08-02

基金资助

江苏省普通高校学术研究生科研创新计划项目(KYZZ16_0495);江苏省农业科技自主创新资金项目(CX(16)1003);江苏省苏北科技专项(BN2016078,BN2016095)

Effects of Antibiotics on Intestinal Microorganisms of Piglets

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  • 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225000, China;
    2. Taicang JinChu Agricultural Development Co., Ltd., Taicang 215400, China;
    3. Huaiyin Agricultural Science Research Institute of Jiangsu Xuhuai Region, Huai'an 223001, China

Received date: 2017-02-10

  Online published: 2017-08-02

摘要

本研究旨在从肠道微生物的角度探究抗生素的作用机制,为今后绿色抗生素替代品添加剂的研究提供理论基础和参考。试验选用6头30日龄的杜×长×大三元杂交去势公猪,随机分为2组,分别为基础饲粮组(不添加抗生素)和抗生素组(基础饲粮中添加0.12%复合抗生素),每组3个重复,每个重复1头猪。试验期为35 d。结果表明:抗生素组肠道微生物物种多样性较基础饲粮组更为丰富;抗生素组厚壁菌门(Firmicute)/拟杆菌门(Bacteroidetes)(F/B)比例较高,因此添加抗生素有利于仔猪体内脂肪的贮存;从肠道微生物的角度考虑,抗生素可能是通过促进L7A_E11、Flexispira、粪球菌属(Coprococcus)等菌属,抑制光岗菌属(Mitsuokella)、巨球菌属(Megasphaera)、柔嫩菌属(Faecalibacterium)等菌属从而发挥预防疾病、提高成活率和促进生长的作用;使用PICRUSt进行功能预测,抗生素在脂类生物合成蛋白质、甲烷代谢、转运蛋白、转录因子、转录机制、RNA转运和脂肪酸生物合成等通路中发挥了重要的作用。由此可见,抗生素可以通过调节仔猪肠道微生物,加速仔猪体内脂肪的贮存,加速断奶仔猪对粗纤维的降解作用,促进短链脂肪酸的生成,从而发挥预防疾病、提高成活率和促进生长等作用。

本文引用格式

刘颖, 王海斌, 周刚, 霍永久, 董丽, 王海飞, 包文斌, 喻礼怀 . 抗生素对仔猪肠道微生物的影响[J]. 动物营养学报, 2017 , 29(8) : 2912 -2922 . DOI: 10.3969/j.issn.1006-267x.2017.08.036

Abstract

The experiment was aimed to explore the mechanism of antibiotics from the perspective of intestinal microbes, and to provide theoretical basis and reference for the future research of green antibiotic alternative. Six Duroc×Landrace×Yorkshire crossbred castrated boars pigs at 30 days of age were selected, and randomly divided into two groups: basal diet group (without antibiotic) and antibiotic group (basal diet supplemented with 0.12% compound antibiotics), each group contained 3 replicates and 1 pigs in each replicate. The experimental lasted for 35 days. The results showed that the intestinal microbial species diversity of antibiotic group was more than that of the basal diet group; the ratio of Firmicute/Bacteroidetes (F/B) of antibiotic group was higher than basal diet group, therefore, the addition of antibiotics was conducive to the storage of fat in piglets; from the perspective of intestinal microflora, antibiotics may through promoting L7A_E11, Flexispira and Coprococcus, etc and inhibiting Mitsuokella, Megasphaera and Faecalibacterium, etc to prevent disease, improve survival rate and promote growth; using PICRUSt to predict the function, antibiotic played an important role in lipid biosynthesis protein, methane metabolism, transport protein, transcription factor, transcription mechanism, RNA transport, fatty acid biosynthesis and other pass way. In conclusion, antibiotics can through regulate intestinal microflora of piglets to accelerate fat storage, accelerate the degradation of coarse fiber in piglets, promote the production of short chain fatty acids, and ultimately help to prevent disease, improve survival rate and promote growth.

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