研究简报 Short communications

丁酸梭菌对断奶仔猪生长性能、肠道屏障功能和血清细胞因子含量的影响

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  • 1. 天津市畜牧兽医研究所, 天津 300381;
    2. 天津市畜禽健康养殖技术工程中心, 天津 300381
李玉鹏(1989—),女,河南鹤壁人,硕士,研究方向为动物营养与饲料。E-mail:773062644@qq.com

收稿日期: 2017-01-09

  网络出版日期: 2017-08-02

基金资助

大北农杨胜先生门生社群项目(B2016008)

Effects of Clostridium butyricum on Growth Performance, Intestinal Barrier Function and Serum Cytokine Contents of Weaned Piglets

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  • 1. Tianjin Institute of Animal Husbandry and Veterinary, Tianjin 300381, China;
    2. Tianjin Livestock and Poultry Health Breeding Technology Engineering Center, Tianjin 300381, China

Received date: 2017-01-09

  Online published: 2017-08-02

摘要

本试验旨在研究饲粮中添加丁酸梭菌对断奶仔猪生长性能、肠道屏障功能和血清细胞因子含量的影响。试验采用单因子设计,选择28日龄、体重相近、健康状况良好的"杜×长×大"断奶仔猪12头,按完全随机区组设计分为2个组,每组6个重复,单栏饲养。对照组饲喂玉米-豆粕型基础饲粮,试验组饲喂基础饲粮+5×105 CFU/g丁酸梭菌。试验预试期3 d,正试期14 d。结果表明:与对照组相比,试验组仔猪平均日增重显著提高7.83%(P<0.05)、料重比降低5.26%(P>0.05);试验组仔猪空肠NOD样受体蛋白(NLRP)3(P<0.05)、NLRP6P<0.05)、NLRP12P<0.01)、封闭蛋白1(claudin-1)(P<0.01)和紧密连接蛋白2(ZO-2)(P<0.05)的mRNA相对表达水平显著或极显著上调,回肠claudin-1和ZO-2的mRNA相对表达水平极显著上调(P<0.01);试验组仔猪空肠和回肠中乳酸杆菌数量显著提高(P<0.05),空肠中大肠杆菌数量降低2.49%(P>0.05),回肠中大肠杆菌数量显著降低(P<0.05);试验组仔猪血清白细胞介素(IL)-1β含量降低5.47%、IL-10含量提高25.43%(P>0.05)。综上所述,饲粮中添加丁酸梭菌能提高仔猪小肠屏障功能,调节机体免疫和肠道菌群平衡,促进仔猪生长。

本文引用格式

李玉鹏, 李海花, 王柳懿, 朱琪, 陈龙宾, 乔家运, 王文杰 . 丁酸梭菌对断奶仔猪生长性能、肠道屏障功能和血清细胞因子含量的影响[J]. 动物营养学报, 2017 , 29(8) : 2961 -2968 . DOI: 10.3969/j.issn.1006-267x.2017.08.041

Abstract

This study was aimed to investigate the effects of Clostridium butyricum on growth performance, intestinal barrier function and serum cytokine contents of weaned piglets. A single factor design was used in this experiment, and 12 Duroc×Landrace×Yorkshire weaned piglets with similar body weight and good health were selected and divided into 2 groups at 28 days of age. Each group was divided into 6 replicates and each pig was fed in single column. Pigs in control group were fed a corn-soybean type basal diet, and those in experimental group were fed the basal diet supplemented with 5×105 CFU/g Clostridium butyricum for 14 days after 3 days adaption. The results showed as follows: compared with the control group, the average daily gain of piglets in experimental group was significantly increased by 7.83% (P<0.05), and the ratio of feed to gain was decreased by 5.26% (P>0.05); the mRNA relative expression levels of NOD like receptor protein (NLRP) 3 (P<0.05), NLRP6 (P<0.05), NLRP12 (P<0.01), claudin-1 (P<0.01) and zonula occludens protein 2 (ZO-2) (P<0.05) were significantly or extremely significantly increased in jejunum and the mRNA relative expression levels of claudin-1 and ZO-2 were extremely significantly increased (P<0.01) in ileum of piglets in experimental group; the number of Lactobacilli in the jejunum and ileum of piglets in experimental group was significantly increased (P<0.05), and the number of Escherichia coli in the jejunum of piglets in experimental group was decreased by 2.49% (P>0.05), while the number of Escherichia coli in the ileum of piglets in experimental group was significantly decreased (P<0.05); the serum interleukin (IL)-1β content of piglets in experimental group was decreased by 5.47% (P>0.05), but the serum IL-10 content of piglets in experimental group was increased by 25.43% (P>0.05). In conclusion, dietary Clostridium butyricum can improve small intestinal barrier function of weaned piglets, regulate body immunity and the balance of intestinal flora, and thus promote weaned piglets growth.

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