动物营养学报 >

2017 , Vol. 29 >Issue 8: 3004 - 3013

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.08.046

研究简报 Short communications

饲粮蛋氨酸水平对湖羊公羔营养物质消化、胃肠道pH及血清指标的影响

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  • 中国农业科学院饲料研究所, 农业部饲料生物技术重点开放实验室, 北京 100081
王杰(1989—),男,山东临沂人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:nkywangjie@163.com

收稿日期: 2017-02-08

  网络出版日期: 2017-08-02

基金资助

国家公益性行业（农业）科研专项（201303143）；国家肉羊产业技术体系建设专项（CARS-39）

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Effects of Dietary Level of Methionine on Nutrient Digestion, Gastrointestinal pH and Serum Indexes of Male Hu Lambs

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  • Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute of Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2017-02-08

  Online published: 2017-08-02

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摘要

本试验旨在研究饲粮蛋氨酸水平对湖羊公羔营养物质消化、胃肠道pH及血清指标的影响。选取12对7日龄断奶的湖羊双胞胎公羔，采用配对试验设计，分为对照（CON）组和低蛋氨酸（LM）组，1对双胞胎羔羊分别分到2个组中。试验分2个阶段进行，第1阶段（8~56日龄），CON组羔羊饲喂基础代乳粉和基础开食料；LM组羔羊饲喂的代乳粉和开食料在CON组基础上分别全部扣除（0.70%和0.40%）额外添加的蛋氨酸，其余营养水平保持一致。第2阶段（57~84日龄），2组羔羊停止饲喂代乳粉且饲粮均为基础开食料。分别在第1阶段结束前（46~55日龄）和第2阶段结束前（74~83日龄）随机选取4对双胞胎羔羊进行消化代谢试验。结果表明：1）56日龄，LM组羔羊对饲粮粗蛋白质、粗脂肪、中性洗涤纤维的表观消化率均显著低于CON组（P<0.05）；84日龄，2组羔羊在营养物质表观消化率上均差异不显著（P>0.05）。2）56日龄，LM组羔羊胃肠道中除十二指肠pH显著低于CON组（P<0.05）外，其他胃肠道pH均差异不显著（P>0.05）；84日龄，2组羔羊在胃肠道pH上均差异不显著（P>0.05）。3）除了56日龄LM组羔羊的生长激素和胰岛素浓度显著低于CON组（P<0.05）外，2组羔羊其他血清指标56和84日龄均无显著性差异（P>0.05）。由此可见，8~56日龄，饲粮低蛋氨酸水平可降低湖羊公羔营养物质表观消化率，以及抑制十二指肠pH和血清中生长激素、胰岛素浓度的增加；57~84日龄，提高饲粮蛋氨酸水平后，湖羊公羔的营养物质表观消化率、胃肠道pH及血清激素指标随之得到补偿。

关键词： 蛋氨酸; 羔羊; 消化代谢; 胃肠道pH; 血清指标

本文引用格式

王杰, 崔凯, 王世琴, 刁其玉, 张乃锋 . 饲粮蛋氨酸水平对湖羊公羔营养物质消化、胃肠道pH及血清指标的影响[J]. 动物营养学报, 2017 , 29(8) : 3004 -3013 . DOI: 10.3969/j.issn.1006-267x.2017.08.046

Abstract

The aim of this study was to assess the effects of dietary level of methionine on the nutrient digestion, gastrointestinal pH and serum indexes of male Hu lambs. Twelve pairs of male Hu twin lambs weaned at 7 days old were selected and divided into two groups (control,CON; low methionine level,LM) with a matched-pairs design. During 8 to 56 days, lambs were fed with and basal starter in CON, while LM group ware fed the basal milk replacer and starter deducting 0.70% and 0.4% methionine compared on control group basis, respectively. During 57 to 84 days, all lambs were fed with basal starter. Two periods of were undertaken at 46 to 55 and 74 to 83 days of age using 4 pairs of twin lambs, respectively. Twelve pairs of male Hu twin lambs weaned at 7 days of age were selected and divided into two groups [control (CON) group and low methionine level (LM) group] with a matched-pairs design, and one pair of lambs were assigned to one of the groups. The experiment consisted of two stages. Stage 1 (8 to 56 days of age), lambs in CON group were fed basal milk replacer and basal starter, while those in LM group ware fed basal milk replacer and basal starter deducting 0.70% and 0.40% methionine on the basis of control group, respectively. Stage 2 (57 to 84 days of age), all lambs were stop feeding milk replacer and fed basal starter. Four twins were selected for digestion and metabolism trials at the end of stages 1 (46 to 55 days of age) and 2 (74 to 83 days of age), respectively. The results showed as follows: 1) at 56 days of age, apparent digestibility of crude protein, ether extract and neutral detergent fiber in LM group were significantly lower than those in CON group (P<0.05); at 84 days of age, no significant differences were observed in apparent digestibility of nutrients between groups (P>0.05). 2) At 56 days of age, no significant differences were observed in other gastrointestinal pH between groups (P>0.05) except duodenum pH, which was significantly lower in LM group than CON group (P<0.05); at 84 days of age, no significant differences were observed in gastrointestinal pH between groups (P>0.05). 3) No significant differences were observed in other serum indexes between groups at 56 and 84 days of age (P>0.05) except growth hormone (GH) and insulin (INS) concentrations at 56 days of age, which were significantly lower in LM group than CON group (P<0.05). In conclusion, at 8 to 56 days of age, low dietary methionine level not only decreases apparent digestibility of nutrients, but also decreases duodenum gastrointestinal pH, GH and INS concentrations of male Hu lambs; at 57 to 84 days of age, subsequent recoveries of nutrient apparent digestibility, gastrointestinal pH and serum hormone indexes appear after the compensation of dietary methionine level.

Key words： methionine; lamb; digestion and metabolism; gastrointestinal pH; serum index

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