硒增强二十二碳六烯酸在脂多糖诱导巨噬细胞炎性反应中的抗炎作用

徐绮嫔, 黄逸馨, 罗正中, 沈留红, 姚学萍, 余树民, 曹随忠

doi:10.3969/j.issn.1006-267x.2017.10.021

动物营养学报 >

2017 , Vol. 29 >Issue 10: 3598 - 3603

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.10.021

分子营养 Molecular Nutrition

硒增强二十二碳六烯酸在脂多糖诱导巨噬细胞炎性反应中的抗炎作用

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四川农业大学动物医学院, 成都 611130

徐绮嫔(1993-),女,广西玉林人,硕士研究生,临床兽医学专业。E-mail:qipinxu@126.com

收稿日期: 2017-03-29

网络出版日期: 2017-09-28

基金资助

四川农业大学学科建设双支计划（3572070）；四川省教育厅重点项目（15ZA0024）

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Selenium Enhances Anti-Inflammatory Effects of Docosahexaenoic Acid on Inflammatory Response in Macrophages Induced by Lipopolysaccharide

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College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2017-03-29

Online published: 2017-09-28

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摘要

为了探究硒是否影响二十二碳六烯酸（DHA）在脂多糖（LPS）诱导巨噬细胞炎性反应中发挥的抗炎作用。小鼠巨噬细胞系RAW264.7细胞分别经10 μg/mL DHA、10 μg/mL DHA+0.05 μmol/L亚硒酸钠、1 μg/mL LPS、10 μg/mL DHA+1 μg/mL LPS、10 μg/mL DHA+1 μg/mL LPS+0.05 μmol/L亚硒酸钠诱导24 h，同时设置无添加的正常组。采用半定量反转录PCR检测细胞肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、白细胞介素6（IL-6）和白细胞介素10（IL-10）mRNA表达量，酶联免疫吸附测定（ELISA）法测定培养液上清液中TNF-α、IL-1β、IL-6和IL-10含量。结果显示，添加亚硒酸钠（0.05 μmol/L）不仅显著或极显著增强了DHA（10 μg/mL）对LPS（1 μg/mL）诱导RAW264.7细胞中促炎细胞因子TNF-α mRNA表达（P<0.05）和IL-1β生成（P<0.01）的抑制作用，还极显著增强了DHA（10 μg/mL）对LPS（1 μg/mL）诱导RAW264.7细胞中抗炎细胞因子IL-10 mRNA表达的促进作用（P<0.01）。由此提示，硒可增强DHA在LPS诱导巨噬细胞炎症反应中的抗炎作用。

关键词： 二十二碳六烯酸; 硒; 巨噬细胞; 抗炎作用

本文引用格式

徐绮嫔, 黄逸馨, 罗正中, 沈留红, 姚学萍, 余树民, 曹随忠 . 硒增强二十二碳六烯酸在脂多糖诱导巨噬细胞炎性反应中的抗炎作用[J]. 动物营养学报, 2017 , 29(10) : 3598 -3603 . DOI: 10.3969/j.issn.1006-267x.2017.10.021

Abstract

In order to investigate whether selenium (Se) affects anti-inflammatory action of docosahexaenoic acid (DHA) on lipopolysaccharide (LPS) induced-macrophage inflammatory response. RAW264.7 cells of mice macrophagic system were treated with 10 μg/mL DHA, 10 μg/mL DHA+0.05 μmol/L sodium selenite, 1 μg/mL LPS, 10 μg/mL DHA+1 μg/mL LPS, 10 μg/mL DHA+1 μg/mL LPS+0.05 μmol/L sodium selenite for 24 h, respectively, a normal group was set up without addition. The mRNA expression levels of tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), interleukin 6 (IL-6) and interleukin 10 (IL-10) in cells were measured by semi-quantitative reverse transcription PCR, and enzyme linked immunosorbent assay (ELISA) method was used to test contents of TNF-α, IL-1β, IL-6 and IL-10 in supernatant of culture medium. The results showed that adding sodium selenite (0.05 μmol/L) not only significantly enhanced the inhibitory effects of DHA (10 μg/mL) on expression of proinflammatory cytokines TNF-α mRNA (P<0.05) and production of IL-1β (P<0.01), but also significantly enhanced the promoting effects of DHA (10 μg/mL) on mRNA expression of anti-inflammatory cytokine IL-10 in RAW264.7 cells induced by LPS (1 μg/mL) (P<0.01). Therefore, Se can enhance anti-inflammatory effects of DHA on inflammatory response in macrophages induced by LPS.

Key words： DHA; selenium; macrophages; anti-inflammatory effect

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