动物营养学报 >

2017 , Vol. 29 >Issue 10: 3627 - 3634

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.10.025

饲料营养 Feed science and technology

植物乳杆菌-泰山松花粉多糖微胶囊对小鼠生长和肠道环境的影响

展开
  • 1. 山东农业大学动物科技学院, 山东省动物生物技术与疫病防治重点实验室, 泰安 271018;
    2. 山东省绿色食品发展中心, 济南 250013;
    3. 山东省动物疫病预防与控制中心, 济南 250022;
    4. 山东新希望六和集团有限公司, 青岛 266061
马宁宁(1989-),女,山东聊城人,硕士研究生,从事微生物与免疫学研究。E-mail:648281470@qq.com

收稿日期: 2017-03-12

  网络出版日期: 2017-09-28

基金资助

山东省现代农业产业技术体系建设经费资助（SDAIT-10-06）；国家重点研发计划项目"牛羊重要疫病诊断与检测新技术研究"（2016YFD0500900）

收起

Preparation of Microcapsules Prepared with Lactobacillus plantarum and Taishan Pine Pollen Polysaccharide and Its Effects on Growth and Intestinal Environment of Mice

Expand
  • 1. Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China;
    2. Shandong Province Green Food Development Center, Ji'nan 250013, China;
    3. Animal Disease Prevention and Control Center of Shandong Province, Ji'nan 250022, China;
    4. Shandong New Hope Liuhe Group Co., Ltd., Qingdao 266061, China

Received date: 2017-03-12

  Online published: 2017-09-28

Fold

摘要

植物乳杆菌作为微生态饲料添加剂饲喂后易受消化道胃酸、胆汁酸和消化酶等不利因素的影响。泰山松花粉多糖（TPPPS）作为一种水溶性植物多糖也易受到胃酸和酶的破坏。为了保护植物乳杆菌和TPPPS的生物活性，并探索二者之间是否存在协同作用。本研究以植物乳杆菌和TPPPS为芯材制备微胶囊，通过正交试验对包埋工艺进行优化，并对最佳条件下制备的微胶囊进行性能检测。选取体重20 g左右无特定病原体（SPF）小鼠48只，随机分为4组，每组12只。4组小鼠每天分别口服饲喂1 mL的植物乳杆菌、植物乳杆菌+0.5% TPPPS、微胶囊、磷酸盐缓冲液（PBS）。饲养试验42 d。每隔1周各组随机取3只小鼠称重记录，剖检后无菌采集肠道组织，测定小肠绒毛和隐窝发育差异及乳酸菌和大肠杆菌定植情况。结果显示：1）微胶囊包埋率达81.6%，粒径为84.3 μm。在模拟胃液中处理120 min植物乳杆菌存活率为62.36%，37℃条件下存储120 d存活率达45.9%。2）与PBS组相比，植物乳杆菌、植物乳杆菌+0.5% TPPPS、微胶囊均能不同程度促进小鼠体重增加，提高小肠中乳酸菌数量，降低大肠杆菌数量，改善小肠绒毛和隐窝发育，微胶囊效果最优。结果提示，植物乳杆菌协同TPPPS使用的益生效果显著优于单独的植物乳杆菌，二者制备的微胶囊能够进一步提高其益生效果。

关键词： 植物乳杆菌; 泰山松花粉多糖; 微胶囊; 小肠绒毛

本文引用格式

马宁宁, 裴宗飞, 朱丽君, 董雯雯, 周建波, 胡莉萍, 魏凯, 黄河, 朱瑞良 . 植物乳杆菌-泰山松花粉多糖微胶囊对小鼠生长和肠道环境的影响[J]. 动物营养学报, 2017 , 29(10) : 3627 -3634 . DOI: 10.3969/j.issn.1006-267x.2017.10.025

Abstract

Lactobacillus plantarum (L. plantarum), as a kind of microecological feed additive, was easy to be affected by adverse factors such as gastric acid, bile acid and digestive enzyme. Taishan pine pollen polysaccharide (TPPPS) was a kind of water soluble plant polysaccharide, which was easy to be damaged by acid and enzyme. In order to protect the biological activity of L. plantarum and TPPPS and to explore whether they have synergistic effect, this experiment prepared microcapsules with L. plantarum and TPPPS as core materials. The microcapsule embedding process was optimized by orthogonal test, and the performance of the microcapsules prepared under the optimum conditions was determined. Forty-eight specific pathogen free (SPF) mice (weighted about 20 g) were randomly divided into 4 groups with 12 mice per group. Mice in different groups were orally fed 1.0 mL of L. plantarum, L. plantarum+0.5% TPPPS, microcapsules and phosphate buffered saline (PBS) (control group), respectively. The feeding trial lasted for 42 d. Every other week, three mice from each group were selected randomly and weighed. After autopsy, intestinal tissues were aseptically collected and measured the changes of villi and crypts in the small intestine, and then the colonization of lactic acid bacteria and Escherichia coli were determined. The results showed as follows:1) encapsulation efficiency of the microcapsules was 81.6% and diameter was 84.3 μm. Survival rate of L. plantarum was 62.36% after 120 min in simulated gastric juice and 45.9% after 120 days storage at 37℃. 2) compared with control group, L. plantarum, L. plantarum +0.5% TPPPS and the microcapsules could increase body weight of mice at different degrees, increased the number of lactic acid bacteria in small intestine, reduced the number of Escherichia coli, improve development of small intestine villi and crypts, and the effects of the microcapsules were the best. The results indicate that probiotic effect of L. plantarum combined with TPPPS is better than that of L. plantarum alone, while the microcapsules prepared with the above combination can further improve their beneficial effects.

Key words： L. plantarum; Taishan pine pollen polysaccharide; microcapsules; small intestine villi

参考文献

[1] SUO C,YIN Y S,WANG X N,et al.Effects of lactobacillus plantarum ZJ316 on pig growth and pork quality[J].BMC Veterinary Research,2012,8(1):89.

[2] SONG D F,ZHU M Y,GU Q.Purification and characterization of Plantaricin ZJ5,a new bacteriocin produced by Lactobacillus plantarum ZJ5[J].PLoS One,2014,9(8):e105549.

[3] ZHU X,ZHAO Y Z,SUN Y L,et al.Purification and characterisation of plantaricin ZJ008,a novel bacteriocin against Staphylococcus,spp.from Lactobacillus plantarum,ZJ008[J].Food Chemistry,2014,165:216-223.

[4] BECK B R,KIM D,JEON J,et al.The effects of combined dietary probiotics Lactococcus lactis,BFE920 and Lactobacillus plantarum FGL0001 on innate immunity and disease resistance in olive flounder (Paralichthys olivaceus)[J].Fish & Shellfish Immunology,2015,42(1):177-183.  

[5] DASH G,RAMAN R P,PANI P K,et al.Evaluation of paraprobiotic applicability of Lactobacillus plantarum in improving the immune response and disease protection in giant freshwater prawn,Macrobrachium rosenbergii (de Man,1879).[J].Fish & Shellfish Immunology,2015,43(1):167-174.  

[6] HANDAN A M,EI-SAYED A F,MAHMOUD M M.Effects of a novel marine probiotic,Lactobacillus plantarum AH 78,on growth performance and immune response of Nile tilapia (Oreochromis niloticus)[J].Journal of Applied Microbiology,2016,120(4):1061-1073.  

[7] GAEDINER G E,O'SULLIVAN E,KELLY J,et al.Comparative survival rates of human-derived probiotic Lactobacillus paracasei and L.salivarius strains during heat treatment and spray drying[J].Applied and Environmental Microbiology,2000,66(6):2605-2612.  

[8] 刘瑛华,赵进宝,吕秀芳.微胶囊包埋技术在益生菌制品中的应用[J].食品与机械,2004,20(2):58-60.

[9] 刘忠杰,徐剑琴.中兽医学[M].北京:中国农业出版社,2003:147-153.

[10] WEI K,SUN Z H,YAN Z G,et al.Effects of Taishan Pinus massoniana pollen polysaccharide on immune response of rabbit haemorrhagic disease tissue inactivated vaccine and on production performance of Rex rabbits[J].Vaccine,2011,29(14):2530-2536.  

[11] CUI G L,ZHONG S X,YANG S F,et al.Effects of Taishan Pinus massoniana pollen polysaccharide on the subunit vaccine of Proteus mirabilis in birds[J].International Journal of Biological Macromolecules,2013,56:94-98.

[12] 李宁.双歧杆菌微胶囊制备工艺及功能特性的研究[D].硕士学位论文.保定:河北农业大学,2007.

[13] SHAH N P.Probiotic bacteria:selective enumeration and survival in dairy foods[J].Journal of Dairy Science,2000,83(4):894-907.  

[14] PARK J K,CHANG H N.Microencapsulation of microbial cells[J].Biotechnology Advances,2000,18(4):303-319.  

[15] EDWARDS A,MUR L A J,GIRDWOOD S E,et al.Coupled cryoconite ecosystem structure-function relationships are revealed by comparing bacterial communities in alpine and Arctic glaciers[J].Fems Microbiology Ecology,2014,89(2):222-237.  

[16] FRANCISCO É C,FRANCO T T,WAGNER R,et al.Assessment of different carbohydrates as exogenous carbon source in cultivation of cyanobacteria[J].Bioprocess and Biosystems Engineering,2014,37(8):1497-1505.  

[17] 周健,王永坤,张纪兵.复合益生素对肉鸡生产性能和肠道菌群的影响[J].中国家禽,2008,30(19):41-42.

[18] AIDY S E,VAN DEN BOGERT B,KLEEREBEZEM M.The small intestine microbiota,nutritional modulation and relevance for health.[J].Current Opinion in Biotechnology,2015,32:14-20.

[19] CASPARY W F.Physiology and pathophysiology of intestinal absorption[J].American Journal of Clinical Nutrition,1992,55(1):299S-308S.

[20] GARRIGA C,HUNTER R R,AMAT C,et al.Heat stress increases apical glucose transport in the chicken jejunum[J].American Journal of Physiology-Regulatory,Integrative and Comparative Physiology,2006,290(1):R195-R201.

[21] 王永芬,乔宏兴,席磊,等.益生菌-黄芪复合生物制剂的制备及其对肉仔鸡的影响[J].中国农业大学学报,2011,16(1):54-59.

[22] 吕鑫.黄芪多糖和益生菌对海兰褐蛋鸡生产性能和免疫功能的影响[J].饲料博览,2014(12):24-28.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2