绿茶粉和绿茶多酚对犬的抗氧化作用

李玉, 陶焕青, 张玉蝶, 徐怡钟, 郝宁, 邹苏萍, 冯士彬, 王希春, 周裔彬, 王一君, 张梁, 吴金节, 宛晓春

doi:10.3969/j.issn.1006-267x.2017.10.037

动物营养学报 >

2017 , Vol. 29 >Issue 10: 3737 - 3749

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.10.037

研究简报 Short communications

绿茶粉和绿茶多酚对犬的抗氧化作用

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1. 安徽农业大学茶树生物学与资源利用国家重点实验室, 合肥 230036;
2. 安徽农业大学动物科技学院, 合肥 230036

李玉(1986-),男,河南固始人,讲师,博士,从事畜禽营养代谢与中毒病研究。E-mail:lydhy2014@ahau.edu.cn

收稿日期: 2017-03-09

网络出版日期: 2017-09-28

基金资助

2016年度安徽省博士后研究人员科研活动经费资助项目（2016B117）；现代农业产业技术体系资助（CARS-19）；茶叶化学与健康领军人才团队引进重大示范项目；国家重点研发计划（2016YFD0501009）

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Antioxidant Effects of Green Tea Powder and Green Tea Polyphenols on Canines

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1. State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China;
2. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received date: 2017-03-09

Online published: 2017-09-28

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摘要

本试验旨在探究绿茶粉及绿茶多酚对犬的抗氧化作用。选用5月龄杂交幼犬15只，随机分为3个组，每组5只。对照组饲喂基础饲粮，绿茶粉组饲喂基础饲粮+1.0%的绿茶粉，绿茶多酚组饲喂基础饲粮+0.25%的绿茶多酚。试验期为84 d。结果表明：与试验开始时相比，试验结束时对照组犬体重增长了162%，绿茶粉组犬体重增长了101%，绿茶多酚组犬体重增长了132%，且绿茶粉组和绿茶多酚组试验结束时犬体重显著低于对照组（P<0.05），比对照组分别降低了61%和30%。试验第84天，绿茶粉组及绿茶多酚组犬血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）、乳酸脱氢酶（LDH）活性均显著或极显著低于对照组（P<0.05或P<0.01），血清超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性极显著高于对照组（P<0.01），血清丙二醛（MDA）含量极显著低于对照组（P<0.01），肝脏抗氧化基因[谷氨酸半胱氨酸连接酶催化亚基（GCLC）、谷氨酸半胱氨酸连接酶修饰亚基（GCLM）、血红素氧合酶1（HO-1）、过氧化氢酶（CAT）]和Ⅱ相解毒酶基因[谷胱甘肽硫转移酶M1（GSTM1）和醌氧化还原酶1（NQO1）]的mRNA相对表达量显著高于对照组（P<0.05）。综上所述，在本试验条件下，在犬饲粮中添加1.0%的绿茶粉或0.25%的绿茶多酚均具有保护肝脏、抗氧化、促进Ⅱ相解毒酶基因表达及控制体重的作用。

关键词： 犬; 绿茶粉; 绿茶多酚; 肝功能; 抗氧化基因; Ⅱ相解毒酶基因

本文引用格式

李玉, 陶焕青, 张玉蝶, 徐怡钟, 郝宁, 邹苏萍, 冯士彬, 王希春, 周裔彬, 王一君, 张梁, 吴金节, 宛晓春 . 绿茶粉和绿茶多酚对犬的抗氧化作用[J]. 动物营养学报, 2017 , 29(10) : 3737 -3749 . DOI: 10.3969/j.issn.1006-267x.2017.10.037

Abstract

The present study was conducted to investigate the antioxidant effects of green tea powder and green tea polyphenols (GPTs) on canines. Fifteen 5-month old canines were selected and randomly divided into three groups (5 canines in each group). The canines in control group were fed a basal diet, the canines in green tea power group were fed the basal diet plus 1.00% green tea power, and the canines in GPTs group were fed the basal diet plus 0.25%GPTs. The feeding experiment lasted for 84 days. The results showed as follows:1) compared with the initial body weight, the final body weight of control group, green tea power group and GTPs group was increased by 162%, 101% and 132%, respectively. The final body weight of green tea power group and GTPs group was significantly lower than that in control group (P<0.05), which was decreased by 61% and 30% compare with control group, respectively. On the 84th day of experiment, the activities of serum alanine transaminase (ALT), aspartate aminotransferase (AST) and lactic dehydrogenase (LDH) in green tea power group and GTPs group were significantly or extremely significantly lower than those in control group (P<0.05 or P<0.01), the activities of serum glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were extremely significantly higher than those in control group (P<0.01), the content of serum malondialdehyde (MDA) was extremely significantly lower than that in control group (P<0.01), and the relative mRNA expression levels of antioxidant genes[glutamate cysteine ligase catalytic subunit (GCLC), glutamate cysteine ligase subunit modification (GCLM), heme oxygenase 1 (HO-1) and catalase (CAT)] and phase Ⅱ detoxification genes[quinone oxidoreductase 1 (NQO1) and glutathione S transferase M1 (GSTM1)] in liver were significantly higher than those in control group (P<0.01). In summary, adding 1.00% green tea powder or 0.25% GTPs in the diet of canines have the effects of liver protection, antioxidant, activating phase Ⅱ detoxification gene expression and controlling body weight under the condition of the present study.

Key words： canines; green tea powder; green tea polyphenols; liver function; antioxidant genes; phase Ⅱ detoxification genes

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