动物营养学报 >

2018 , Vol. 30 >Issue 1: 138 - 146

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.01.018

水产营养 Aquaculture Nutrition

饥饿时间对管角螺生长、生化组成、消化酶活性和抗氧化指标的影响

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  • 宁波大学海洋学院, 宁波 315211
王双健(1992-),男,江西九江人,硕士研究生,从事水产养殖研究。E-mail:792854990@qq.com

收稿日期: 2017-06-19

  网络出版日期: 2018-01-04

基金资助

国家星火级计划项目（2015GA701040）；象山县农业种子种苗项目（2015C2013）

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Effects of Starvation Time on Growth, Biochemical Composition, Enzyme Activities and Antioxidant Indexes of Hemifusus tuba Gmelin

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  • School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received date: 2017-06-19

  Online published: 2018-01-04

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摘要

为了探明不同饥饿时间对管角螺生长、生化组成、消化酶活性和抗氧化指标的影响，选取体重为（28.33±1.27） g的管角螺360只，随机分为6组（每组设3个重复，每个重复放养20只），设置饥饿时间分别为0（对照组）、5、10、20、30、40 d，在水温29~31℃条件下进行饥饿试验。结果显示：饥饿时间对管角螺的存活率、壳高和壳宽均无显著影响（P>0.05），各组的存活率均为100%；随着饥饿时间的延长，管角螺的体重逐渐下降，特别是饥饿20~40 d时，体重减少12.73%~21.14%。除饥饿40 d外，饥饿时间对足肌中水分和粗蛋白质含量的影响不显著（P>0.05）；随着饥饿时间的延长，足肌中粗脂肪含量逐渐下降，粗灰分含量逐渐上升。随着饥饿时间的延长，足肌糖原含量显著下降（P<0.05），肝糖原含量呈下降趋势，除饥饿10与20 d组间以及饥饿20和30 d组间无显著差异（P>0.05）外，其他组间差异显著（P<0.05）。随着饥饿时间的延长，足肌饱和脂肪酸和单不饱和脂肪酸的含量逐渐下降，而多不饱和脂肪酸的含量相对上升；偶数碳原子个数的脂肪酸含量逐渐下降，而奇数碳原子个数的脂肪酸含量却相对上升。肝胰腺脂肪酶和胰蛋白酶活性在饥饿5 d时显著高于对照组（P<0.05），在饥饿40 d时显著低于对照组（P<0.05）；肝胰腺淀粉酶活性在饥饿10 d时显著高于对照组和饥饿5 d组（P<0.05），在饥饿40 d时显著低于对照组（P<0.05）。肝胰腺超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）活性在饥饿5~30 d内随着饥饿时间的延长而上升，并显著高于对照组（P<0.05），而饥饿40 d时却显著低于对照组（P<0.05）；肝胰腺丙二醛（MDA）含量在饥饿30 d前无显著差异（P>0.05），而在饥饿30 d后则显著升高（P<0.05）。以上结果表明：管角螺耐饥饿，但在高温（29~31℃）下饥饿30 d后其体内生化指标均发生了巨大的变化，表明其体内已经发生了饥饿胁迫。

关键词： 管角螺; 饥饿胁迫; 存活率; 生长; 营养成分; 酶活性

本文引用格式

王双健, 丁玉惠, 江茂旺, 蒋霞敏, 韩庆喜 . 饥饿时间对管角螺生长、生化组成、消化酶活性和抗氧化指标的影响[J]. 动物营养学报, 2018 , 30(1) : 138 -146 . DOI: 10.3969/j.issn.1006-267x.2018.01.018

Abstract

This experiment was conducted to reveal the effects of different starvation time on growth, biochemical composition, enzyme activities and antioxidant indexes of Hemifusus tuba Gmelin. A total of 360 Hemifusus tuba Gmelin with the body weight of (28.33±1.27) g were randomly divided into 6 groups with 3 replicates per group and 20 snails per replicate, and the snails in the 6 groups had different starvation time:0 (control group), 5, 10, 20, 30 and 40 days at the water temperature of 29 to 31℃. The results showed that the survival rate, shell height and shell width were not significant affected by starvation time (P>0.05), and the survival rate of each group was 100%. But the weight was gradually decreased with the starvation time increasing, especially for without feeding for 20 to 40 days, and the weight loss was 12.73% to 21.14%. The moisture and crude protein contents in pedal muscle of Hemifusus tuba Gmelin were not significant affected by starvation time (P>0.05) (except starvation 40 days). The content of ether extract in pedal muscle was gradually decreased along with the increase of starvation time, but the content of ash in pedal muscle was gradually increased. With the starvation time increasing, pedal muscle glycogen content was significantly decreased (P<0.05), and liver glycogen content showed a downward trend which was significantly different among different groups (P<0.05) except groups with 10 and 20 days of starvation and groups with 20 and 30 days of starvation. With the starvation time increasing, the contents of saturated fatty acid and monounsaturated fatty acid in pedal muscle were gradually declined, while the content of unsaturated fatty acid was relatively rose. With the starvation time increasing, the content of fatty acids of even number of carbon atoms was gradually decreased, while the number of fatty acids of odd number of carbon atoms was relatively increased. Compared with the control group, the activities of lipase and protease in hepatopancreas of Hemifusus tuba Gmelin in group with 5 days of starvation were significantly increased (P<0.05), and it in the group with 40 days of starvation was significantly decreased (P<0.05). the activity of amylase in hepatopancreas of Hemifusus tuba Gmelin in the group with 10 days of starvation was significantly higher than that of the control group and the group with 10 days of starvation (P<0.05), and it in the group with 40 days of starvation was significantly lower than that of the control group (P<0.05). The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in hepatopancreas were rose with the increase of starvation time in 5 to 30 days of starvation time, and were significantly higher than those in the control group (P<0.05), while those in the group with 40 days of starvation were significantly lower than those in the control group (P<0.05). The content of malondialdehyde (MDA) in hepatopancreas was not significantly different among groups less than 30 days of starvation (P>0.05), but it was significantly higher than that in the groups with more than 30 days of starvation (P<0.05). The above results indicated that the Hemifusus tuba Gmelin are extremely insusceptible to hunger, but their biochemical markers have changed dramatically after 30 days of starvation at high temperatures (29 to 31℃), indicating that starvation stress has occurred in their body.

Key words： Hemifusus tuba Gmelin; starvation time; survival rate; growth; nutritional components; enzyme activity

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