动物营养学报 >

2018 , Vol. 30 >Issue 1: 326 - 335

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.01.039

研究简报 Short communications

干酪乳杆菌对北京黑猪保育阶段生长性能及肠道菌群的影响

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  • 1. 北京市农林科学院畜牧兽医研究所, 北京 100097;
    2. 北京黑六牧业科技有限公司, 北京 102211
王四新(1969-),女,北京人,高级畜牧师,本科,主要从事动物营养与饲料研究。E-mail:1005892033@qq.com

收稿日期: 2017-06-29

  网络出版日期: 2018-01-04

基金资助

北京市科技计划课题（Z141100002614002）；生猪产业技术体系北京市创新团队项目（BAIC02-2017）；北京市农林科学院科技创新能力建设专项（KJCX20161503）

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Effects of Lactobacillus casei on Growth Performance and Intestinal Microbiota of Beijing Black Pigs in Nursery Stage

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  • 1. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
    2. Beijing Heiliu Animal Husbandry Science and Technology Co., Ltd., Beijing 102211, China

Received date: 2017-06-29

  Online published: 2018-01-04

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摘要

本试验旨在探讨饲粮中添加干酪乳杆菌对北京黑猪保育阶段生长性能及肠道菌群的影响。选择（35±2）日龄、体重（7.53±0.21） kg的北京黑猪仔猪120头，随机分成3组，分别为对照组、干酪乳杆菌组和金霉素组，每组4个重复，每个重复10头。对照组试验猪饲喂基础饲粮（不添加干酪乳杆菌和金霉素）；干酪乳杆菌组试验猪饲喂在基础饲粮中添加干酪乳杆菌活菌冻干制剂的饲料，每千克饲料中有效活菌数在4.0×109 CFU；金霉素组试验猪饲喂在基础饲粮中添加金霉素预混剂的饲料，每千克饲料含金霉素75 mg。试验期30 d，在试验第28天时采集新鲜粪样，用于16S rRNA的V3~V4区测序。结果显示：1）与对照组相比，干酪乳杆菌组和金霉素组的平均日增重分别提高12.71%（P<0.05）和8.58%（P<0.05），料重比分别降低7.34%（P<0.05）和4.52%（P>0.05）。2）通过对粪样菌群ACE指数、Chao1指数、Shannon指数和Simpson指数进行分析，发现干酪乳杆菌组肠道菌群丰富度和多样性均高于对照组和金霉素组。3）3组粪样中共含有17个菌门，206个菌属，对照组、干酪乳杆菌组和金霉素组粪样中分别含有198、200和197个菌属。由此可见，在北京黑猪保育阶段饲粮中添加干酪乳杆菌可改善仔猪肠道内菌群结构，进而提高生长性能。

关键词： 北京黑猪; 生长性能; 肠道菌群; 干酪乳杆菌; 金霉素

本文引用格式

王四新, 季海峰, 石国华, 刘辉, 王红卫, 张董燕, 王晶, 张伟, 王雅民 . 干酪乳杆菌对北京黑猪保育阶段生长性能及肠道菌群的影响[J]. 动物营养学报, 2018 , 30(1) : 326 -335 . DOI: 10.3969/j.issn.1006-267x.2018.01.039

Abstract

This experiment was conducted to study the effects of Lactobacillus casei on growth performance and intestinal microflora of Beijing black pigs in nursery stage. A total of 120 weaned piglets for Beijing black pigs with the age of (35±2) days and average body weight of (7.53±0.21) kg were randomly distributed into 3 groups with 4 replicates per group and 10 piglets per replicate, comprising of control group, Lactobacillus casei group and chlortetracycline group. The piglets in the control group were fed a basal diet (without Lactobacillus casei or chlortetracycline), the piglets in the Lactobacillus casei group were fed the basal diet supplemented with living bacteria freeze dry preparation of Lactobacillus casei, and the living bacteria count was 4.0×109 CFU/kg diet, and the piglets in the chlortetracycline group were fed the basal diet supplemented with chlortetracycline premix, and the chlortetracycline content was 75 mg/kg diet. The experiment lasted for 30 d. At the 28th day of the experiment, the fresh feces were collected and used for sequencing of the V3-V4 hypervariable region of the 16S rRNA. The results showed as follows:1) compared with the control group, the average daily gain of Lactobacillus casei group and chlortetracycline group was significantly increased by 12.71% (P<0.05) and 8.58% (P<0.05), the feed/gain was decreased by 7.34% (P<0.05) and 4.52% (P>0.05), respectively. 2) Lactobacillus casei group increased the species richness and diversity of microbiota, as indicated by the ACE index, Chao1 index, Shannon index and Simpson index, compared with the control group and the chlortetracycline group. 3) Seventeen phyla and 206 genera were contained by three groups of feces, and 198, 200 and 197 genera were found in the control group, Lactobacillus casei group and chlortetracycline group, respectively. The results indicate that Lactobacillus casei supplementation in the diet has beneficial effects on the improvement of intestinal microflora structure and growth performance of Beijing black pigs in nursery stage.

Key words： Beijing black pigs; growth performance; intestinal microbiota; Lactobacillus casei; chlortetracycline

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