本试验旨在研究饲粮不同维生素E水平对三黄肉鸡免疫功能、组织α-生育酚沉积及脂蛋白脂酶（LPL）、脂肪酸结合蛋白基因表达的影响。将256只80日龄广西三黄肉鸡随机分为4组，各组分别在基础饲粮中添加0（对照）、50、100、150 mg/kg的维生素E，每组4个重复，每重复16只。预试期5 d，正试期35 d。结果表明，与对照组相比：1）饲粮添加50 mg/kg维生素E显著提高血清中免疫球蛋白A含量（P<0.05）；饲粮添加150 mg/kg维生素E显著提高血清中免疫球蛋白M含量（P<0.05）；饲粮添加不同水平的维生素E对脾脏指数和血清中白细胞介素2、肿瘤坏死因子α、γ干扰素及免疫球蛋白G含量均无显著影响（P>0.05）。2）饲粮添加50、100及150 mg/kg维生素E均能显著提高肝脏α-生育酚含量（P<0.05）；饲粮添加150 mg/kg维生素E显著提高胸肌α-生育酚含量（P<0.05）；饲粮添加不同水平维生素E对腿肌α-生育酚含量无显著影响（P>0.05）。3）饲粮添加100和150 mg/kg维生素E均能显著提高肝脏LPL基因表达量（P<0.05）；饲粮添加150 mg/kg维生素E能显著提高肝脏心脏型脂肪酸结合蛋白（H-FABP）基因表达量（P<0.05）；饲粮添加50、100及150 mg/kg维生素E均能显著提高肝脏肝脏型脂肪酸结合蛋白（L-FABP）基因表达量（P<0.05）；饲粮添加不同水平维生素E对肝脏脂肪细胞型脂肪酸结合蛋白（A-FABP）基因表达量无显著影响（P>0.05）。4）饲粮维生素E添加水平与广西三黄肉鸡肝脏LPL、H-FABP、L-FABP基因表达量呈显著正相关（P<0.05）。综合得出，饲粮添加高水平（150 mg/kg）的维生素E可以改善广西三黄肉鸡的免疫功能，提高组织中α-生育酚的沉积，调节肝脏LPL、H-FABP、L-FABP基因表达量，从而影响机体的脂质代谢。

This experiment was to investigate the effects of different dietary vitamin E levels on immune function, α-tocopherol deposition and gene expressions of lipoprotein lipase (LPL) and fatty acid-binding proteins in tissues of Sanhuang chicken. A total of 256 Guangxi Sanhuang chickens at 80 days of age were randomly divided into 4 groups with 4 replicates per group and 16 chickens per replicate. Vitamin E at 0 (control), 50, 100 and 150 mg/kg was added to a basal diet of different groups, respectively. Pre-experiment lasted for 5 d, and experiment lasted for 35 d. The results showed that compared with control group:1) the content of immunoglobulin A (IgA) in serum was significantly increased by dietary supplementation of 50 mg/kg vitamin E (P<0.05); the content of immunoglobulin M (IgM) in serum was significantly increased by dietary supplementation of 50 mg/kg vitamin E (P<0.05); dietary supplementation of different levels of vitamin E had no significant effects on spleen index, and the contents of interleukin-2, tumor necrosis factor-α, interferon-γ and immunoglobulin G (IgG) in serum (P>0.05). 2) The content of α-tocopherol in liver was significantly increased by dietary supplementation of 50, 100 and 150 mg/kg vitamin D (P<0.05); the content of α-tocopherol in breast was significantly increased by dietary supplementation of 150 mg/kg vitamin D (P<0.05); dietary supplementation of different levels of vitamin E had no significant effects on the content of α-tocopherol in thigh (P>0.05). 3) The expression level of LPL gene in liver was significantly increased by dietary supplementation of 100 and 150 mg/kg vitamin D (P<0.05); the expression level of heart type-fatty acid-binding protein (H-FABP) gene in liver was significantly increased by dietary supplementation of 150 mg/kg vitamin D (P<0.05); the expression level of liver type-fatty acid-binding protein (L-FABP) gene in liver was significantly increased by dietary supplementation of 50, 100 and 150 mg/kg vitamin D (P<0.05); dietary supplementation of different levels of vitamin E had no significant effects on the expression level of adipocyte type-fatty acid-binding protein (A-FABP) gene (P>0.05). 4) There was a significant positive correlation between dietary vitamin E level and expression levels of LPL, H-FABP and L-FABP genes in liver of Guangxi Sanhuang chicken (P<0.05). In summary, it is suggested that the supplementation of a high level (150 mg/kg) of vitamin E in diet can improve immune function, increase α-tocopherol deposition in tissues, and regulate expression levels of LPL, H-FABP and L-FABP genes in liver of Guangxi Sanhuang chicken, which may has impact on the body's fat metabolism.