动物营养学报 >

2018 , Vol. 30 >Issue 3: 1019 - 1026

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.03.026

分子营养 Molecular Nutrition

早期断奶对仔猪糖原含量以及肝脏糖异生和糖酵解相关基因表达的影响

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  • 1. 浙江省农业科学院农产品质量标准研究所, 杭州 310021;
    2. 江西省畜牧技术推广站, 南昌 330046;
    3. 贵州大学动物科学学院, 贵阳 550025
杨华(1972-),男,浙江绍兴人,高级畜牧师,硕士,主要从事单胃动物营养研究。E-mail:yanghua@mail.zaas.ac.cn

收稿日期: 2017-09-11

  网络出版日期: 2018-03-05

基金资助

国家自然科学基金（31402083）；浙江省自然科学基金重点项目（LZ15C170001）

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Effects of Early Weaning on Glycogen Concent and Expression of Hepatic Genes Associated with Gluconeogenesis and Glycolysis in Piglets

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  • 1. Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    2. Jiangxi Animal Husbandry Technology Extension Station, Nanchang 330046, China;
    3. College of Animal Science, Guizhou University, Guiyang 550025, China

Received date: 2017-09-11

  Online published: 2018-03-05

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摘要

本试验旨在探讨早期断奶对仔猪机体糖代谢的影响。选择4窝18日龄健康仔猪，从每窝中各选4头共计16头仔猪，称重后其中4头直接屠宰用于取样（血液、肝脏、背最长肌），另外12头进行断奶处理（断奶组）；每窝剩余仔猪由母猪继续哺乳（哺乳组）。在断奶后第1天、第3天和第7天从断奶组和哺乳组各取4头仔猪测定平均日增重、血清中葡萄糖和乳酸含量、肝脏和背最长肌中糖原含量以及肝脏中丙酮酸激酶活性与糖代谢相关基因的相对表达水平。结果表明：与哺乳组仔猪相比，在早期断奶阶段（断奶后1~7 d），断奶组仔猪的平均日增重显著下降（P<0.05）；在断奶后第1天、第3天和第7天，断奶组仔猪血清中葡萄糖含量分别降低了8.33%（P>0.05）、17.81%（P<0.05）和20.99%（P<0.05），血清中乳酸含量分别上升了16.83%（P<0.05）、22.75%（P<0.05）和12.06%（P>0.05），肝糖原含量分别降低了24.94%（P<0.05）、48.99%（P<0.05）和36.51%（P<0.05），肌糖原含量分别降低了44.49%（P<0.05）、39.68%（P<0.05）和25.52%（P<0.05）；断奶组仔猪肝脏糖酵解关键酶丙酮酸激酶活性及其基因相对表达水平在断奶后第3天和第7天均显著降低（P<0.05），而肝脏糖异生相关酶线粒体磷酸烯醇式丙酮酸羧激酶（断奶后第1天）、胞质磷酸烯醇式丙酮酸羧激酶（断奶后第1天、第3天和第7天）和葡萄糖-6-磷酸酶的基因相对表达水平（断奶后第1天和第3天）均显著提高（P<0.05）。由此可见，早期断奶使得仔猪断奶后前期阶段血清葡萄糖含量、肝糖原和肌糖原含量降低，肝脏的糖异生作用被激活而糖酵解作用被抑制以维持体内的糖代谢平衡。

关键词： 仔猪; 早期断奶; 糖原; 糖异生; 糖酵解

本文引用格式

杨华, 易松强, 徐娥, 陈小敏, 戴宝玲, 肖英平 . 早期断奶对仔猪糖原含量以及肝脏糖异生和糖酵解相关基因表达的影响[J]. 动物营养学报, 2018 , 30(3) : 1019 -1026 . DOI: 10.3969/j.issn.1006-267x.2018.03.026

Abstract

The objective of this study was to elucidate the effects of early weaning on glycometabolism of piglets. Sixteen 18-day-old piglets were selected from 4 litters with each litter selecting 4 piglets. After weighted, four piglets were euthanized directly and serum, liver and longissimus muscle samples were collected, while the other 12 piglets were segregated from the sows as the weaned group. The rest of the piglets in the same litter continued being breastfed as suckling group. Four piglets were weighted on day 1, 3 and 7 postweaning in weaned group and age-match suckling group, respectively, and then used to measure the contents of glucose and lactate in serum, the content of glycogen in liver and longissimus muscle, the activity of pyruvate kinase and the relative expression level of genes associated glycometabolism in liver. The results showed that the average daily gain of piglets in weaned group was significantly decreased compared with suckling group at early weaning stage (1 to 7 days postweaning) (P<0.05). Compared with suckling piglets, serum glucose content of weaned piglets was decreased by 8.33% (P>0.05), 17.81% (P<0.05) and 20.99% (P<0.05) on day 1, 3 and 7 postweaning, respectively; whereas serum lactate content of weaned piglet was increased by 16.83% (P<0.05), 22.75% (P<0.05) and 12.06% (P<0.05), respectively. Additionally, the liver glycogen content of weaned piglets was decreased by 24.94% (P<0.05), 48.99% (P<0.05) and 36.51% (P<0.05), respectively; and muscle glycogen content of weaned piglets was decreased by 44.49% (P<0.05), 39.68% (P<0.05) and 25.52% (P<0.05), respectively. Compared with suckling piglets, the activity of pyruvate kinase, a key glycolytic enzyme, and its gene relative expression level in liver of weaned piglets were significantly decreased on day 3 and 7 postweaning (P<0.05), while the relative expression levels of glycogen related enzymes, such as mitochondrial phosphoenolpyruvate carboxykinase (day 1 postweaning), cytoplasmic phosphoenolpyruvate carboxykinase (day 1, 3 and 7 postweaning) and glucose-6 phosphatase (day 1 and 3 postweaning) in liver of weaned piglets were significantly increased (P<0.05). In conclusion, the contents of serum glucose, liver glycogen and muscle glycogen are decreased in piglets by early weaning, and the gluconeogenesis is activated and the glycolysis is suppressed in liver of early weaned piglets to ensure the balance of glycometabolism in body.

Key words： piglets; early weaning; glycogen; gluconeogenesis; glycolysis

参考文献

[1] MOESER A J,KLOK C V,RYAN K A,et al.Stress signaling pathways activated by weaning mediate intestinal dysfunction in the pig[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2007,292(1):173-181.  

[2] WIJTTEN P J,VAN DER MEULEN J,VERSTEGEN M W.Intestinal barrier function and absorption in pigs after weaning:a review[J].British Journal of Nutrition,2011,105(7):967-981.  

[3] 张文飞,刘苹苹,管武太,等.饲粮中添加卵黄抗体对断奶仔猪生长性能、血清生化指标、肠道形态及肠道微生物菌群的影响[J].动物营养学报,2017,29(1):271-279.

[4] NAFIKOV R A,BEITZ D C.Carbohydrate and lipid metabolism in farm animals[J].The Journal of Nutrition,2007,137(3):702-705.  

[5] SHE P X,BURGESS S C,SHIOTA M,et al.Mechanisms by which liver-specific PEPCK knockout mice preserve euglycemia during starvation[J].Diabetes,2003,52(7):1649-1654.  

[6] HANSON R W,GARBER A J.Phosphoenol pyruvate carboxykinase Ⅰ:its role in gluconeogenesis[J].American Journal of Clinical Nutrtition,1972,25(10):1010-1021.  

[7] 王龙生,杨华,代兵,等.早期断奶对仔猪血清、肌肉及肝脏氨基酸代谢的影响[J].中国畜牧杂志,2016,52(21):65-71.

[8] XIAO Y P,WU T X,HONG Q H,et al.Response to weaning and dietary L-glutamine supplementation:Metabolomic analysis in piglet by gas chromatography/mass spectrometry[J].Journal of Zhejiang University:Science B,2012,13(7):567-578.  

[9] JIAO L F,KE Y L,XIAO K,et al.Effects of cello-oligosaccharide on intestinal microbiota and epithelial barrier function of weanling pigs[J].Journal of Animal Science,2015,93(3):1157-1164.  

[10] WU G,KNABE D A.Free and protein-bound amino acids in sow's colostrum and milk[J].The Journal of Nutrition,1994,124(3):415-424.  

[11] CHENG Z B,LI D F,GE C R,et al.Polyamines in sow colostrum and milk at different stages of lactation[J].Animal Science,2006,82(1):95-99.

[12] WANG J J,CHEN L X,LI P,et al.Gene expression is altered in piglet small intestine by weaning and dietary glutamine supplementation[J].The Journal of Nutrition,2008,138(6):1025-1032.  

[13] 孔祥杰,姜海龙,蔡维北,等.黄芪茎叶对断奶仔猪盲肠微生物区系的影响[J].中国畜牧杂志,2017,53(3):117-120,124.

[14] HAMPSON D J,KIDDER D E.Influence of creep feeding and weaning on brush borsh border enzyme activities in the piglet small intestine[J].Research in Veterinary Science,1986,40(1):24-31.

[15] PIÉ S,LALL?S J P,BLAZY F,et al.Weaning is associated with an upregulation of expression of inflammatory cytokines in the intestine of piglets[J].The Journal of Nutrition,2004,134(3):641-647.  

[16] XIAO Y P,LI X Y,WU T X,et al.Effects of dietary glutamine supplementation on nutrient absorption and activity of enzymes involved in glutamine metabolism and energy production in the jejunum of weaned piglets[J].Journal of Animal and Veterinary Advance,2012,11(9):1441-1449.  

[17] VAN POELJE P D,POTTER S C,CHANDRAMOULI V C,et al.Inhibition of fructosel,6-bisphoshatase reduces excessive endogenous glucose production and attenuates hyperglycemia in Zuekerdiabetic fatty rats[J].Diabetes,2006,55(6):1747-1754.  

[18] LE DIVIDICH J,ESNAULT T,LYNCH B,et al.Effect of colostral fat level on fat deposition and plasma metabolites in the newborn pig[J].Journal of Animal Science,1991,69(6):2480-2488.  
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