屎肠球菌SF68对断奶仔猪生长性能、血常规和血清生化指标及抗氧化性能的影响

冯宝宝, 方伟, 封飞飞, 赵国琦, 霍永久

doi:10.3969/j.issn.1006-267x.2018.03.038

动物营养学报 >

2018 , Vol. 30 >Issue 3: 1125 - 1135

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.03.038

饲料营养 Feed science and technology

屎肠球菌SF68对断奶仔猪生长性能、血常规和血清生化指标及抗氧化性能的影响

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扬州大学动物科学与技术学院, 扬州 225009

冯宝宝(1994-),女,河北沧州人,硕士研究生,从事猪营养、生产与环境研究。E-mail:fengbaobao1994@163.com

收稿日期: 2017-10-02

网络出版日期: 2018-03-05

基金资助

江苏省重点研发计划现代农业项目（BE2015329）；扬州市-扬州大学产学研合作专项（YZ2016256）

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Effects of Enterococcus faecalis SF68 on Growth Performance, Blood Routine and Serum Biochemical Indexes and Antioxidant Ability of Weaned Piglets

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College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2017-10-02

Online published: 2018-03-05

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摘要

本试验旨在研究饲粮中添加屎肠球菌SF68对断奶仔猪生长性能、血常规和血清生化指标及抗氧化性能的影响。选取90头21日龄体重[（6.57±0.08）kg]相近的"杜洛克×长白猪×大约克"三元杂交去势断奶仔猪，随机分为5个组，每组3个重复，每个重复6头猪（公母各占1/2）。对照组（CON组）饲喂基础饲粮，抗生素组（ANT组）在基础饲粮中添加0.007 5%的金霉素和0.02%的硫酸抗敌素，抗生素+低剂量屎肠球菌组（ALEF组）在基础饲粮中添加0.007 5%的金霉素、0.02%的硫酸抗敌素和0.01%的屎肠球菌SF68，低剂量屎肠球菌组（LEF组）在基础饲粮中添加0.01%的屎肠球菌SF68，高剂量屎肠球菌组（HEF组）在基础饲粮中添加0.05%的屎肠球菌SF68。试验期21 d。结果表明：1）各组之间断奶仔猪的平均日增重（ADG）、平均日采食量（ADFI）、料重比（F/G）和腹泻指数无显著差异（P>0.05）。ALEF、LEF组腹泻指数相比CON组分别降低了65%和67%。2）LEF组血液中平均血红蛋白含量（MCH）、平均红细胞体积（MCV）和淋巴细胞百分比显著或极显著高于CON组（P<0.05或P<0.01）。3）ALEF组血清中总胆汁酸（TBA）含量显著低于HEF组（P<0.05），ALEF组血清中乳酸脱氢酶（LDH）活性显著高于HEF组（P<0.05），LEF组血清中胆碱酯酶（CHE）活性显著高于CON组和HEF组（P<0.05）。4）ALEF组和LEF组血清中过氧化氢（H₂O₂）含量显著或极显著高于CON组、ANT组和HEF组（P<0.05或P<0.01），ALEF组和HEF组肝脏中超氧化物歧化酶（SOD）活性、总抗氧化能力（T-AOC）显著高于CON组（P<0.05）。ANT组、ALEF组、LEF组和HEF组空肠中H₂O₂含量显著或极显著低于CON组（P<0.05或P<0.01），ALEF组空肠中SOD活性显著高于ANT组、LEF组和HEF组（P<0.05）。ANT组、ALEF组、LEF组和HEF组回肠中SOD活性显著或极显著高于CON组（P<0.05或P<0.01），丙二醇（MDA）含量显著或极显著低于CON组（P<0.05或P<0.01）。由此可见，饲粮中添加屎肠球菌SF68可提高断奶仔猪生长性能，减少腹泻，改善血液生理生化指标，增强动物机体氧气运输的能力，提高机体免疫力，调节脂代谢和能量代谢，增强机体的抗氧化性能，并且LEF组作用效果要优于HEF组，但是混合添加抗生素和屎肠球菌SF68要比单独添加屎肠球菌作用效果好。

关键词： 屎肠球菌; 断奶仔猪; 生长性能; 血常规指标; 血清生化指标; 抗氧化性能

本文引用格式

冯宝宝, 方伟, 封飞飞, 赵国琦, 霍永久 . 屎肠球菌SF68对断奶仔猪生长性能、血常规和血清生化指标及抗氧化性能的影响[J]. 动物营养学报, 2018 , 30(3) : 1125 -1135 . DOI: 10.3969/j.issn.1006-267x.2018.03.038

Abstract

This experiment was conducted to stufy the effects of dietary Enterococcus faecalis SF68 on growth performance, blood routine and serum biochemical indexes and antioxidant ability of weaned piglets. Ninety castrated "Duroc×Landrace×Yorkshire" weaned piglets with similar body weight[(6.57±0.08) kg] were randomly assigned to 5 groups with 3 replicates per group and 6 pigs per replicate (half male and half female). Pigs in the control group (CON group) were fed a basal diet, pigs in the antibiotic group (ANT group) were fed the basal diet supplemented with 0.007 5% chlortetracycline and 0.02% colistin sulfate, pigs in the antibiotic +low dose Enterococcus faecalis group (ALEF group) were fed the basal diet supplemented with 0.007 5% chlortetracycline, 0.02% colistin sulfate and 0.01% Enterococcus faecium SF68, pigs in the low dose Enterococcus faecalis group (LEF group) were fed the basal diet supplemented with 0.01% Enterococcus faecium SF68, and pigs in the high dose Enterococcus faecalis group (HEF group) were fed the basal diet supplemented 0.05% Enterococcus faecium SF68. The experiment lasted for 21 days. The results showed as follows:1) there were no significant differences on the average daily gain (ADG), average daily feed intake (ADFI), ratio of feed to gain (F/G) and diarrhea index of weaned piglets among all groups (P>0.05). The diarrhea indexes of ALEF and LEF groups were decreased by 65% and 67% compared with the CON group, respectively. 2) The mean corpuscular hemoglobin (MCH), average red blood cell volume (MCV) and lymphocytes percentage in blood of LEF group were significantly higher than those of CON group (P<0.05 or P<0.01). 3) The serum total bile acid (TBA) content of ALEF group was significantly lower than that of HEF group (P<0.05), the serum lactate dehydrogenase (LDH) activity of ALEF group was significantly higher than that of HEF group (P<0.05), the serum cholinesterase (CHE) activity of LEF group was significantly higher than that of CON and HEF groups (P<0.05). 4) The serum hydrogen peroxide (H₂O₂) content of ALEF and LEF groups was significantly higher than that of CON, ANT and HEF groups (P<0.05 or P<0.01), the superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC) in liver of ALEF and HEF groups were significantly higher than those of CON group (P<0.05). The jejunum H₂O₂ content of ANT, ALEF, LEF and HEF groups was significantly lower than that of CON group (P<0.05 or P<0.01), the jejunum SOD activity of ALEF group was significantly higher than that of ANT, LEF and HEF groups (P<0.05). The ileum SOD activity of ANT, ALEF, LEF and HEF groups was significantly higher than that of CON group (P<0.05 or P<0.01), the ileum malondialdehyde (MDA) content of ANT, ALEF, LEF and HEF groups was significantly lower than that of CON group (P<0.05 or P<0.01). The results indicate that the addition of Enterococcus faecalis SF68 in the basal diet can improve the growth performance of weaned piglets, reduce the diarrhea, improve the physiological and biochemical indexes of blood, enhance the ability of animal body oxygen transport and improve the immunity of the body, regulate lipid metabolism and energy metabolism, and enhance the antioxidant ability of the body, and the effect of LEF group is better than HEF group, but the mixed addition of antibiotics and Enterococcus faecium SF68 is better than that of the alone addition of Enterococcus faecalis.

Key words： Enterococcus faecalis; weaned piglets; growth performance; blood routine indexes; serum biochemical indexes; antioxidant ability

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