动物营养学报 >

2018 , Vol. 30 >Issue 4: 1522 - 1529

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.04.037

饲料营养 Feed science and technology

饲料中添加低聚木糖对凡纳滨对虾幼虾消化酶活力、肠道形态及细菌数量的影响

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  • 1. 广东省农业科学院动物科学研究所, 广州 510640;
    2. 农业部华南动物营养与饲料重点实验室, 广州 510640;
    3. 广东省畜禽育种与营养研究重点实验室, 广州 510640;
    4. 广州飞禧特生物科技有限公司, 广州 510640
陈晓瑛(1987-),女,福建龙岩人,硕士,从事水产动物营养与饲料学研究。E-mail:524852502@qq.com

收稿日期: 2017-10-11

  网络出版日期: 2018-04-03

基金资助

广东省科技厅省重点实验室项目（2014B030301054）；广东省农业科学院学科团队建设项目（水产营养与饲料学科团队）；广东省级现代农业产业技术推广体系建设项目（2017LM1082，2017LM1083）

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Effects of Dietary Xylo-Oligosaccharides on Digestive Enzymes Activities, Intestinal Morphology and Bacteria Numbers of Juvenile Litopenaeus vannamei

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  • 1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture, Guangzhou 510640, China;
    3. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China;
    4. Guangzhou Fishtech Biotechnology Co. Ltd., Guangzhou 510640, China

Received date: 2017-10-11

  Online published: 2018-04-03

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摘要

本试验旨在研究饲料中添加低聚木糖（XOS）对凡纳滨对虾（Litopenaeus vannamei）消化酶活力、肠道形态及细菌数量的影响。试验选用平均体重为（0.67±0.02）g的凡纳滨对虾幼虾800尾，随机分成4组，每组4个重复，每个重复50尾虾。对照组投喂基础饲料，试验组分别投喂在基础饲料中添加200、400和600 mg/kg XOS的试验饲料。试验期为42 d。结果表明：1）与对照组相比，试验组对虾肠道淀粉酶活力和肝胰腺蛋白酶活力显著提高（P<0.05），200 mg/kg组肠道和肝胰腺蛋白酶活力、400 mg/kg组肠道和肝胰腺脂肪酶活力、400 mg/kg组胃中蛋白酶活力显著升高（P<0.05）。2）与对照组相比，400 mg/kg组对虾肝胰腺总蛋白含量、肠壁厚度和肠绒毛高度均显著升高（P<0.05）。3）与对照组相比，试验组对虾肠道总菌和双歧杆菌（Bifidobacterium）数量显著升高（P<0.05），肠道弧菌（Vibrio）数量显著降低（P<0.05）。由此可见，饲料中添加XOS能提高凡纳滨对虾幼虾消化酶活力，改善肠道形态，提高肠道总菌和双歧杆菌数量，降低弧菌数量。

关键词： 低聚木糖; 凡纳滨对虾; 消化酶活力; 肠道形态; 肠道细菌

本文引用格式

陈晓瑛, 王国霞, 孙育平, 陈冰, 莫文艳, 赵红霞, 曹俊明, 黄燕华 . 饲料中添加低聚木糖对凡纳滨对虾幼虾消化酶活力、肠道形态及细菌数量的影响[J]. 动物营养学报, 2018 , 30(4) : 1522 -1529 . DOI: 10.3969/j.issn.1006-267x.2018.04.037

Abstract

This experiment was conducted to investigate the effects of dietary xylo-oligosaccharides (XOS) on digestive enzyme activities, intestinal morphology and bacteria numbers of juvenile Litopenaeus vannamei. A total of 800 juvenile Litopenaeus vannamei with an average body weight of (0.67±0.02) g were randomly assigned to 4 groups with 4 replicates per group and 50 shrimps per replicate. Shrimps in the control group were fed a basal diet, and others in the experimental groups were fed the basal diets supplemented with 200, 400, and 600 mg/kg XOS, respectively. The experiment lasted for 42 days. The results showed as follows:1) compared with the control group, the intestinal amylase activity and hepatopancreas protease activity of shrimps in experimental groups were significantly increased (P<0.05), the intestinal and hepatopancreas protease activity in 200 mg/kg group, the intestinal and hepatopancreas lipase activity in 400 mg/kg group, and stomach protease activity in 400 mg/kg group were significantly increased (P<0.05). 2) Compared with the control group, the hepatopancreas total protein content, intestinal wall thickness and intestinal villus height of shrimps in 400 mg/kg group were significantly increased (P<0.05). 3) Compared with the control group, the intestinal total bacteria and Bifidobacteria numbers of shrimps in experimental groups were significantly increased (P<0.05), while the intestinal vibrio number was significantly decreased (P<0.05). In conclusion, dietary XOS can improve digestive enzyme activities and intestinal morphology, increase intestinal total bacteria and Bifidobacteria numbers, decrease intestinal Vibria number of juvenile Litopenaeus vanname.

Key words： xylo-oligosaccharides; Litopenaeus vannamei; digestive enzyme activities; intestinal morphology; intestinal bacteria

参考文献

[1] MITSUOKA T.Prebiotics and intestinal flora[J].Bioscience & Microflora,2010,21(1):3-12.

[2] FRANCIS R J,BROUNS F.Immune-stimulating and gut health-promoting properties of Shortchain Fructo-oligosaccharides[J].Nutrition Reviews,2002,60(10):326-334.  

[3] DE MAESSCHALCK C,EECKHAUT V,MAERTENS L,et al.Effects of xylo-oligosaccharides on broiler chicken performance and microbiota[J].Applied and Environmental Microbiology,2015,81(17):5880-5888.  

[4] 石宁.地衣芽孢杆菌和低聚木糖及乳酸对肉鸡肠绒组织及肠道菌群的影响[D].硕士学位论文.郑州:河南工业大学,2010.

[5] 杨海英,杨在宾,杨维仁,等.益生素和低聚木糖对断奶仔猪生产性能、消化酶活性、血液指标和肠道微生物的影响[J].中国兽医学报,2009,29(7):914-919.

[6] 刘亚利,刑贞召,翟蒲荣.低聚木糖对仔猪生产性能和肠道菌群的影响[J].西北农业学报,2009,18(6):63-65.

[7] 李君华,刘佳亮,曹学彬,等.饲料中添加低聚木糖对仿刺参幼参生长性能、肠道消化酶活力和免疫力的影响[J].动物营养学报,2016,28(8):2534-2541.

[8] 姚东林,邹青,刘文斌,等.地衣芽孢杆菌和低聚木糖对草鱼生长性能、肠道菌群和消化酶活性的影响[J].大连海洋大学学报,2014,29(2):136-140.

[9] 熊娟,吴志新,张朋,等.投喂低聚木糖对草鱼肠道菌群的影响[J].微生物学通报,2011,38(3):317-321.

[10] 张荣斌,曹俊明,黄燕华,等.低聚木糖对奥尼罗非鱼肠道形态、菌群组成和抗嗜水气单胞菌感染的影响[J].上海海洋大学学报,2012,21(2):233-240.

[11] 胡毅.凡纳滨对虾饲料配方优化及几种饲料添加剂的应用[D].硕士学位论文.青岛:中国海洋大学,2007.

[12] 陈晓瑛,曹俊明,黄燕华,等.饲料中添加低聚木糖对凡纳滨对虾幼虾生长性能、非特异性免疫力、抗氧化功能及抗对虾白斑综合征病毒能力的影响[J].动物营养学报,2014,26(8):2397-2407.

[13] 刘玉梅,朱谨钊.对虾消化酶的研究[J].海洋科学,1984,8(5):144-159.

[14] 潘鲁青,王克行.中国对虾幼体消化酶活力的实验研究[J].水产学报,1997,21(1):26-31.

[15] 朱俭.生物化学实验[M].上海:上海科学技术出版社,1981:190-193.

[16] BRADFORD M M.A rapid and sensitive method for the quantitation microgram quantities of protein utilizing the principle of protein-dye binding[J].Analytical Biochemistry,1976,72(1/2):248-254.

[17] 李继秋.对虾微生态制剂的研究和应用[D].博士学位论文.青岛:中国海洋大学,2004.

[18] 刘波,谢骏,刘文斌,等.地衣芽孢杆菌与低聚木糖对异育银鲫消化酶活性、肠道菌群及生长的影响[J].大连水产学院学报,2006,21(4):336-340.

[19] 强俊,王辉,李瑞伟,等.低聚木糖对奥尼罗非鱼幼鱼生长、体成分和消化酶活力的影响[J].淡水渔业,2009,39(6):63-68.

[20] SUGITA H,SHIBUYA K,SHIMOOKA H,et al.Antibacterial abilities of intestinal bacteria in freshwater cultured fish[J].Aquaculture,1996,145(1/2):195-203.

[21] 高丽松.消化生理与保健[M].北京:中国医药科技出版社,1998:173-230.

[22] 胡彩虹,占秀安.果寡糖对肉鸡消化机能的影响[J].中国粮油学报,2003,18(1):49-54.

[23] 黄燕华,王国霞,刘襄河,等.低聚木糖对凡纳滨对虾生长及消化道主要消化酶活性的影响[J].华南农业大学学报,2010,31(3):61-64.

[24] 肖明松,王志耕,崔峰,等.果寡糖和糖萜素对中华鳖生长性能及免疫功能的影响[J].水利渔业,2004,24(3):20-22.

[25] 熊沈学,刘文斌,方星星.低聚木糖梯度添加对异育银鲫生长及肠道消化酶活性的影响[J].畜牧与兽医,2005,37(10):23-24.

[26] 刘爱君,冷向军,李小勤,等.甘露寡糖对奥尼罗非鱼(Oreochromis niloticus×O. aureus)生长、肠道结构和非特异性免疫的影响[J].浙江大学学报(农业与生命科学版),2009,35(3):329-336.

[27] 田娟,孙立威,文华,等.壳寡糖对吉富罗非鱼幼鱼生长性能、前肠组织结构及肠道主要菌群的影响[J].中国水产科学,2013,20(3):561-568.

[28] 徐介民.壳聚糖对草鱼生长影响的研究[D].硕士学位论文.长沙:湖南农业大学,2008.

[29] 曾丽萍.两种多糖对齐口裂腹鱼肠道消化酶、形态及菌群的影响[D].硕士学位论文.雅安:四川农业大学,2015.

[30] CASPARY F W.Physiology and pathophysiology of intestinal absorption[J].The American Journal of Clinical Nutrition,1992,55(1S):299S-308S.

[31] MOURÃOA J L,PINHEIROA V,ALVESA A,et al.Effect of mannan oligosaccharides on the performance,intestinal morphology and cecal fermentation of fattening rabbits[J].Animal Feed Science and Technology,2006,126(1/2):107-120.

[32] DUAN Y F,ZHANG Y,DONG H B,et al.Effect of dietary Clostridium butyricum on growth,intestine health status and resistance to ammonia stress in Pacific white shrimp Litopenaeus vannamei[J].Fish & Shellfish Immunology,2017,65:25-33.

[33] 刘小华,李舒梅,熊跃玲.短链脂肪酸对肠道功效及其机制的研究进展[J].肠外与肠内营养,2012,19(1):56-58.

[34] 占秀安,胡彩虹,许梓荣.果寡糖对肉鸡生长、肠道菌群和肠形态的影响[J].中国兽医学报,2003,23(2):196-198.

[35] 王继成,潘灵辉,李淑云,等.低聚木糖对断奶仔猪生产性能、肠道菌群及免疫水平影响的研究[J].中国畜牧兽医,2006,33(5):3-7.

[36] SUO H Q,LIN L U,XU G H,et al.Effectiveness of dietary xylo-oligosaccharides for broilers fed a conventional corn-soybean meal diet[J].Journal of Integrative Agriculture,2015,14(10):2050-2057.  

[37] 刘卫东,宋素芳,程璞.甘露寡糖和益生菌对肉仔鸡生产性能和肠道菌群的影响[J].家畜生态学报,2011,32(1):32-35.

[38] 温若竹,江芸,刘泽兴,等.甘露寡糖对肉仔鸡肠道微生物区系发育的影响[J].浙江大学学报(农业与生命科学版),2011,37(1):83-90.

[39] 梁超.饲料中添加二甲酸钾、低聚木糖和菊粉对刺参的生长、免疫和抗灿烂弧菌感染能力的影响[D].硕士学位论文.青岛:中国海洋大学,2011.

[40] 盛清凯,姚惠源.低聚糖对肠道菌群的调节机理[J].动物科学与动物医学,2002,19(2):35-38.

[41] PESTOVA M I,CLIFT R E,VICKERS R J,et al.Effect of weaning and dietary galactose supplementation on digesta glycoproteins in pigs[J].science of food and agriculture,2000,80(13):1918-1924.  
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