饲料营养 Feed science and technology

丁酸梭菌抑制脂多糖致急性应激大鼠肠道损伤的效果研究

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  • 1. 浙江省农业科学院, 畜牧兽医研究所, 杭州 310021;
    2. 浙江省农业科学院, 植物保护与微生物研究所, 杭州 310021
吴杰(1989-),男,浙江台州人,硕士研究生,从事动物营养与饲料研究。E-mail:brandjerry@163.com

收稿日期: 2017-10-16

  网络出版日期: 2018-04-03

基金资助

农业部公益性行业专项"饲料中抗生素替代品关键研究与示范"(201403047);浙江省重点研发项目(2018C02044);现代农业产业技术体系建设专项资金

Inhibitory Effect of Clostridium butyricum on Intestinal Damage of Lipopolysaccharide-Induced Acute Stress Rats

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  • 1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    2. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received date: 2017-10-16

  Online published: 2018-04-03

摘要

本试验旨在研究丁酸梭菌(Clostridium butyricum)对健康大鼠生长性能及脂多糖(LPS)致急性应激大鼠肠道结构、肠道二糖酶活性及肠道炎症的影响。选取36只雄性SD大鼠,按体重分为对照组、LPS组和LPS+丁酸梭菌组,每4只大鼠1笼,每组3笼。对照组和LPS组饲喂基础饲粮,LPS+丁酸梭菌组在基础饲粮的基础上添加0.05%丁酸梭菌。试验第40天,LPS组、LPS+丁酸梭菌组大鼠称重后按照4 mg/kg BW的剂量腹腔注射LPS(LPS浓度为1.5 mg/mL,注射量在1.0 mL左右),对照组大鼠腹腔注射1 mL生理盐水,3 h后处死采样。结果表明:1)丁酸梭菌对健康大鼠末重、平均日增重、平均日采食量、料重比未产生显著影响(P<0.05)。2)与对照组相比,注射LPS显著增加了十二指肠隐窝深度(P<0.05),显著降低了十二指肠绒隐比、空肠黏膜厚度以及空肠、回肠上皮淋巴细胞数(P<0.05)。与LPS组相比,预防性饲喂丁酸梭菌能显著降低十二指肠隐窝深度(P<0.05),显著提高空肠黏膜厚度及上皮淋巴细胞数(P<0.05)。3)与对照组相比,注射LPS显著降低了十二指肠蔗糖酶和空肠麦芽糖酶活性(P<0.05)。与LPS组相比,预防性饲喂丁酸梭菌能抑制LPS对十二指肠蔗糖酶活性的降低,LPS+丁酸梭菌组十二指肠蔗糖酶活性与对照组相比差异不显著(P>0.05)。4)与对照组相比,注射LPS显著增加了空肠及回肠髓过氧化物酶(MPO)活性及白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)含量(P<0.05)。与LPS组相比,预防性饲喂丁酸梭菌显著降低了空肠与回肠MPO活性及空肠IL-6、TNF-α含量(P<0.05),LPS+丁酸梭菌组回肠IL-6、TNF-α含量虽无显著降低(P>0.05),但较对照组也未见显著增加(P>0.05)。由此得出,丁酸梭菌对健康大鼠生长的无负面影响,给大鼠预防性饲喂丁酸梭菌可抑制LPS急性应激下LPS对肠道蔗糖酶活性的降低,缓解对肠道黏膜结构的破坏,提高肠道免疫功能,降低肠道炎症。

本文引用格式

吴杰, 邓波, 李孝辉, 徐子伟 . 丁酸梭菌抑制脂多糖致急性应激大鼠肠道损伤的效果研究[J]. 动物营养学报, 2018 , 30(4) : 1530 -1537 . DOI: 10.3969/j.issn.1006-267x.2018.04.038

Abstract

This experiment was conducted to investigate the effects of Clostridium butyricum on the growth performance of healthy rats and the intestinal structure, intestinal disaccharidase activities and intestinal inflammation of lipopolysaccharide (LPS)-induced acute stress rats. Thirty six male SD rats were randomly allocated to control group, LPS group, LPS + Clostridium butyricum group. Control group and LPS group were fed a basal diet, LPS + Clostridium butyricum group were fed the basal diet with the addition of 0.05% Clostridium butyricum. On day 40 of experiment, LPS group and LPS + Clostridium butyricum group were given an intraperitoneal injection of 4 mg/kg BW LPS (LPS concentration was 1.5 mg/mL and the injection volume about 1 mL). Rats in control group were given an intraperitoneal injection of 1 mL saline solution. Three hours later, all rats were sacrificed to collect samples. The results were as follows:1) Clostridium butyricum showed no significant effects on final weight, average daily gain, average daily feed intake and feed/gain of healthy rats (P>0.05). 2) Injection of LPS significantly increased crypt depth in duodenum and significantly decreased villus height/crypt depth in duodenum, mucosal thickness in jejunum, intraepithelial lymphocyte (IEL) count in jejunum and ileum as compared with control group (P<0.05). Preventive feeding Clostridium butyricum significantly decreased crypt depth in duodenum and significantly increased mucosal thickness and IEL count in jejunum as compared with LPS group (P<0.05). 3) Injection of LPS significantly decreased sucrase activity in duodenum and maltase activity in jejunum as compared with control group (P<0.05). Preventive feeding Clostridium butyricum suppressed the decrease of sucrase activity in duodenum and the sucrase activity in duodenum in LPS + Clostridium butyricum group showed no significant difference as compared with control group (P>0.05). 4) Injection of LPS significantly increased of myeloperoxidase (MPO) activity, the contents interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in jejunum and ileum as compared with control group (P<0.05). Preventive feeding Clostridium butyricum significantly decreased MPO activity in jejunum and ileum, and the contents of IL-6 and TNF-α in jejunum as compared with LPS group (P<0.05). Meantime, Clostridium butyricum suppressed the increase of IL-6 and TNF-α contents in ileum and showed no significant difference to control group (P>0.05). In conclusion, Clostridium butyricum shows no negative effects on growth performance of healthy rats. For LPS-induced acute stress rats, pretreatment of Clostridium butyricum can suppress the decrease of intestinal sucrase activity, ameliorate the damage of intestinal mucosal structure, improve intestinal immune function and decrease intestinal inflammation.

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