壳寡糖对小鼠生长性能、血清免疫和抗氧化指标的影响

张南南, 李兰兰, 张晓昀, 闻正顺, 邹晓庭

doi:10.3969/j.issn.1006-267x.2018.04.039

动物营养学报 >

2018 , Vol. 30 >Issue 4: 1538 - 1546

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.04.039

饲料营养 Feed science and technology

壳寡糖对小鼠生长性能、血清免疫和抗氧化指标的影响

展开

1. 浙江大学饲料科学研究所, 农业部动物营养与饲料科学重点实验室, 杭州 310058;
2. 浙江海洋大学食品与医药学院, 舟山 316022

张南南(1988-),女,山东菏泽人,硕士研究生,动物营养与饲料科学专业。E-mail:929812084@qq.com

收稿日期: 2017-10-16

网络出版日期: 2018-04-03

基金资助

浙江省自然科学基金（LY17C170001，LQ13C200002）

收起

Effects of Chitosan Oligosaccharide on Growth Performance, Serum Immune and Antioxidant Indexes of Mice

Expand

1. Key Laboratory of Animal Nutrition and Feed Science of Agriculture, Feed Science Institute, Zhejiang University, Hangzhou 310058, China;
2. College of Food and Medicine, Zhejiang Ocean University, Zhoushan 316022, China

Received date: 2017-10-16

Online published: 2018-04-03

Fold

摘要

本试验旨在研究灌胃不同剂量壳寡糖（COS）对小鼠生长性能和血清免疫球蛋白含量、细胞因子水平、抗氧化酶活性的影响。选取60只5周龄无特定病原体（SPF）级ICR雄性小鼠，随机分为6组，包括1个对照组和5个试验组，每组10只。5个试验组小鼠分别按照0.2、0.4、0.6、0.8和1.0 g/kg BW的剂量灌胃COS（溶解于生理盐水），对照组灌服等体积的生理盐水。试验期6周。结果表明：1）灌胃不同剂量COS对小鼠体重无显著影响（P>0.05）。2）灌胃0.6 g/kg BW COS可显著提高小鼠胸腺指数（P<0.05），较对照组提高了32.87%。3）灌胃0.6 g/kg BW COS可显著提高小鼠血清免疫球蛋白A（IgA）和免疫球蛋白M（IgM）含量（P<0.05），较对照组分别提高了12.14%和20.69%。4）灌胃不同剂量COS对小鼠血清白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、白细胞介素-1β（IL-1β）、转化生长因子-β（TGF-β）和肿瘤坏死因子-α（TNF-α）水平均无显著影响（P>0.05）。5）灌胃0.6 g/kg BW COS可显著提高小鼠血清中髓过氧化物酶（MPO）活性（P<0.05），较对照组提高了27.61%；灌胃0.8 g/kg BW COS可显著提高小鼠血清过氧化物酶（POD）活性（P<0.05），较对照组提高了45.08%。综上，在本试验条件下，COS可促进小鼠免疫器官发育并具有调节血清免疫和抗氧化指标的功能。

关键词： 壳寡糖; ICR小鼠; 生长性能; 免疫球蛋白; 细胞因子; 抗氧化酶

本文引用格式

张南南, 李兰兰, 张晓昀, 闻正顺, 邹晓庭 . 壳寡糖对小鼠生长性能、血清免疫和抗氧化指标的影响[J]. 动物营养学报, 2018 , 30(4) : 1538 -1546 . DOI: 10.3969/j.issn.1006-267x.2018.04.039

Abstract

This experiment was carried out to investigate the effects of gavage administration of different doses of chitosan oligosaccharides (COS) on growth performance, serum immunoglobulins contents, cytokines levels and antioxidant enzyme activities of mice. Sixty 5-week-old specific pathogen-free (SPF) ICR male mice were selected and randomly divided into 6 groups, including 1 control group and 5 experimental groups with 10 mice each. The mice in 5 experimental groups were given gavage administration of COS (dissolved in normal saline) at levels of 0.2, 0.4, 0.6, 0.8 and 1.0 g/kg BW, and the mice in control group were given gavage administration of the same volume of normal saline. The experiment lasted for 6 weeks. The results showed as follows:1) gavage administration of different doses of COS had no significant influence on body weight of mice (P>0.05). 2) Thymus index was significantly increased by gavage administration of 0.6 g/kg BW COS, and it was increased by 32.87% compared with the control group (P<0.05). 3)Gavage administration of 0.6 g/kg BW COS significantly increased the contents of serum immunoglobulin A (IgA) and immunoglobulin (IgM), and they were increased by 12.14% and 20.69%, respectively, compared with the control group (P<0.05). 4) No significant differences were found in serum interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-1β (IL-1β), transforming growth factor-β (TGF-β) and tumour necrosis factor-α (TNF-α) by gavage administration of different doses of COS (P>0.05). 5)The activity of myeloperoxidase (MPO) was significantly increased by gavage administration of 0.6 g/kg BW COS, and it was increased by 27.61% compared with the control group (P<0.05). The activity of peroxidase (POD) was significantly increased by gavage administration of 0.8 g/kg BW COS, and it was increased by 45.08% compared with the control group (P<0.05). In summary, under the conditions of this experiment, COS has function of promoting the development of immune organs and regulating serum immune and antioxidant indexes of mice.

Key words： chitosan oligosaccharides; ICR mice; growth performance; immunoglobulin; cytokine; antioxidant enzyme

参考文献

[1] ELALA N M A,RAGAA N M.Eubiotic effect of a dietary acidifier (potassium diformate) on the health status of cultured Oreochromis niloticus[J].Journal of Advanced Research,2015,6(4):621-629.

[2] WAN J,JIANG F,XU Q S,et al.New insights into the role of chitosan oligosaccharide in enhancing growth performance,antioxidant capacity,immunity and intestinal development of weaned pigs[J].RSC Advances,2017,7(16):9669-9679.

[3] 谭利伟,管昶,周鲁宁,等.壳寡糖在动物营养中的研究与应用[J].中国畜牧杂志,2016,52(17):91-97.

[4] ZHOU T X,CHO J H,KIM I H.Effects of supplementation of chito-oligosaccharide on the growth performance,nutrient digestibility,blood characteristics and appearance of diarrhea in weanling pigs[J].Livestock Science,2012,144(3):263-268.

[5] 孙立威,文华,蒋明,等.壳寡糖对吉富罗非鱼幼鱼生长性能、非特异性免疫及血液学指标的影响[J].广东海洋大学学报,2011,31(3):43-49.

[6] YOON H J,MOON M E,IM S Y,et al.Chitosan oligosaccharide (COS) inhibits LPS-induced inflammatory effects in RAW 264.7 macrophage cells[J].Biochemical & Biophysical Research Communications,2007,358(3):954-959.

[7] MA P,LIU H T,WEI P,et al.Chitosan oligosaccharides inhibit LPS-induced over-expression of IL-6 and TNF-α in RAW264.7 macrophage cells through blockade of mitogen-activated protein kinase (MAPK) and PI3K/Akt signaling pathways[J].Carbohydrate Polymers,2011,84(4):1391-1398.

[8] 麻攀,于炜婷,魏鹏,等.壳寡糖作用于巨噬细胞的机制初探[J].天然产物研究与开发,2012(5):660-662.

[9] 汤峻,黄皓源,周乐礼,等.壳寡糖对妊娠后期母猪免疫功能和仔猪初生重的影响[J].安徽农业科学,2016,44(6):174-176.

[10] 张玲,陈代文,杨继文,等.壳寡糖对环磷酰胺应激仔猪生产性能和免疫功能的影响[J].中国畜牧杂志,2013,49(17):58-62.

[11] 李阳,常文环,张姝,等.饲粮添加壳寡糖和干酪乳杆菌对肉鸡生长性能、肌肉品质及抗氧化性能的影响[J].动物营养学报,2016,28(5):1450-1461.

[12] 乔丽红,赵颖,倪红玉,等.低聚壳聚糖对断奶仔猪血清生化指标、抗氧化性能和粪便微生物的影响[J].粮食与饲料工业,2013(3):47-50.

[13] 徐小龙,周鲁宁,张斌,等.壳寡糖替代抗生素对肉仔鸡生长性能及免疫功能的影响[J].饲料广角,2016(17):33-37.

[14] 黄钦成,申光荣,谭北平,等.饲料中添加壳寡糖和/或霉菌毒素吸附剂对凡纳滨对虾生长性能、非特异性免疫力及抗病力的影响[J].动物营养学报,2017,29(11):4036-4047.

[15] 侯改凤,李瑞,黄兴国.寡糖的免疫调节作用及其在动物生产中的应用[J].中国饲料,2015(4):28-30.

[16] CHEN B,LIU L F,XU H Y,et al.Effectiveness of immune therapy combined with chemotherapy on the immune function and recurrence rate of cervical cancer[J].Experimental and Therapeutic Medicine,2015,9(3):1063-1067.

[17] YU G P,CHEN G Q,HUANG B,et al.Effect of early enteral nutrition on postoperative nutritional status and immune function in elderly patients with esophageal cancer or cardiac cancer[J].Chinese Journal of Cancer Research,2013,25(3):299-305.

[18] 邓阳勇,伍参荣,杨旭丽,等.芦荟多糖对衰老小鼠胸腺组织结构的影响[J].中南医学科学杂志,2011,39(6):626-628.

[19] DANG Y B,LI S,WANG W X,et al.The effects of chitosan oligosaccharide on the activation of murine spleen CD11c⁺ dendritic cells via Toll-like receptor 4[J].Carbohydrate Polymers,2011,83(3):1075-1081.

[20] 崔健,康鹏天.壳寡糖的生理功能及在水产养殖中的作用[J].甘肃畜牧兽医,2015,45(4):21-24.

[21] 陈虹,臧素敏,侯伟革.不同浓度壳寡糖对蛋用鹌鹑免疫功能的影响[J].中国畜牧兽医,2013,40(1):108-111.

[22] 占秀安,胡彩虹,许梓荣.果寡糖对肉鸡生长、肠道菌群和肠形态的影响[J].中国兽医学报,2003,23(2):196-198.

[23] 孙忠保,阎宏,马永军.寡糖在动物生产中的应用[J].农业科学研究,2004,25(4):80-84.

[24] 孟晓,王纪亭,万文菊,等.低分子质量壳寡糖对蛋鸡生产性能、蛋品质、血清生化指标、盲肠微生物数量及脾脏白细胞介素-2、肿瘤坏死因子-α基因表达的影响[J].动物营养学报,2017,29(5):1590-1599.

[25] DENG X Z,LI X J,LIU P,et al.Effect of chito-oligosaccharide supplementation on immunity in broiler chickens[J].Asian-Australasian Journal of Animal Sciences,2008,21(11):1651-1658.

[26] WALSH A M,SWEENEY T,BAHAR B,et al.The effect of chitooligosaccharide supplementation on intestinal morphology,selected microbial populations,volatile fatty acid concentrations and immune gene expression in the weaned pig[J].Animal,2012,6(10):1620-1626.

[27] 贾培媛,李海霞,巫亚俊,等.壳寡糖对猪蓝耳病灭活疫苗的佐剂活性研究[C]//中国酶工程与糖生物工程学术研讨会论文摘要集.镇江:中国微生物学会酶工程专业委员会,2015:66-68.

[28] GELFANOVA V,LAI Y,GELFANOV V,et al.Modulation of cytokine responses of murine CD8⁺ αβ intestinal intraepithelial lymphocytes by IL-4 and IL-12[J].Journal of Biomedical Science,1999,6(4):269-276.

[29] BERG D J,ZHANG J,WEINSTOCK J V,et al.Rapid development of colitis in NSAID-treated IL-10-deficient mice[J].Gastroenterology,2002,123(5):1527-1542.

[30] BERG D J,DAVIDSON N,KVHN R,et al.Enterocolitis and colon cancer in interleukin-10-deficient mice are associated with aberrant cytokine production and CD4⁺ TH1-like responses[J].Journal of Clinical Investigation,1996,98(4):1010-1020.

[31] SUN X Y,YANG H,NOSE K,et al.Decline in intestinal mucosal IL-10 expression and decreased intestinal barrier function in a mouse model of total parenteral nutrition[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2008,294(1):G139-G147.

[32] XIE W M,XU P X,LIU Q.Antioxidant activity of water-soluble chitosan derivatives[J].Bioorganic & Medicinal Chemistry Letters,2001,11(13):1699-1701.

[33] 王振伟,申森.超声波辅助酶法制备壳寡糖及抗氧化活性研究[J].中国食品添加剂,2014(4):70-75.

[34] 张吉,刘洪涛,李秀英,等.壳寡糖对自由基的清除及对N9小胶质细胞的保护作用[J].食品科学,2010,31(7):81-85.

[35] JOODI G,ANSARI N,KHODAGHOLI F.Chitooligosaccharide-mediated neuroprotection is associated with modulation of Hsps expression and reduction of MAPK phosphorylation[J].International Journal of Biological Macromolecules,2011,48(5):726-735.

[36] 孙立威,文华,蒋明,等.壳寡糖对吉富罗非鱼幼鱼生长性能、非特异性免疫及血液学指标的影响[J].广东海洋大学学报,2011,31(3):43-49.

[37] 李振达,陈小娥,廖智,等.壳寡糖对三疣梭子蟹免疫力的影响[J].浙江海洋学院学报(自然科学版),2011,30(1):27-32.

[38] 李桂峰,钱沛锋,孙际佳,等.维生素C对胡子鲶血清免疫相关酶活性的影响[J].大连水产学院报,2004,19(4):301-305.

[39] 钟佳,刘进辉,肖定福,等.壳寡糖及其衍生物的抗氧化活性[J].动物医学进展,2015,36(7):118-121.

[40] FENG T,DU Y M,LI J,et al.Antioxidant activity of half N-acetylated water-soluble chitosan in vitro[J].European Food Research and Technology,2007,225(1):133-138.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们