动物营养学报 >

2018 , Vol. 30 >Issue 4: 1574 - 1581

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.04.043

研究简报 Short communications

不同氮能比饲粮对妊娠环江香猪羊水和尿囊液生化参数的影响

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  • 1. 河南科技大学动物科技学院, 洛阳 471003;
    2. 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙 410125;
    3. 中国科学院环江喀斯特农业生态试验站, 香猪研究中心, 环江 547100
赵越(1991-),女,河南商丘人,硕士研究生,从事猪的孕体发育调控研究。E-mail:285762564@qq.com

收稿日期: 2017-09-30

  网络出版日期: 2018-04-03

基金资助

国家自然科学基金面上项目（31572421，31270044）；中科院"西部之光"人才培养计划重点项目

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Effects of Different Nitrogen/Energy Diets on Biochemical Parameters in Amniotic Fluid and Allantoic Fluid of Pregnant Huanjiang Mini-Pigs

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  • 1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;
    2. Key Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    3. Research Center of Mini-Pig, Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Sciences, Huanjiang 547100, China

Received date: 2017-09-30

  Online published: 2018-04-03

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摘要

本试验旨在研究不同氮能比饲粮对妊娠环江香猪羊水和尿囊液生化参数的影响。选用首次妊娠环江香猪48头，随机分为2组，每组8个重复（栏），每个重复3头。配种后2组母猪分别饲喂高氮能比饲粮（消化能为14.73 MJ/kg，粗蛋白质含量为13.11%，氮能比为0.89）和低氮能比饲粮（消化能为12.24 MJ/kg，粗蛋白质含量为9.77%，氮能比为0.80），于妊娠第45、75和110天每栏取1头母猪屠宰，收集每窝中最大体重、中间体重和最小体重胎猪对应的羊水和尿囊液，测定生化参数。结果表明：在相同妊娠时间和相同标准体重条件下，与饲喂低氮能比饲粮相比，饲喂高氮能比饲粮可显著增加尿囊液中妊娠75 d最小体重胎猪碱性磷酸酶活性（P<0.05），显著降低妊娠75 d中间、最小体重胎猪和妊娠110 d最小体重胎猪谷草转氨酶活性，妊娠45、75 d最大体重胎猪尿酸含量，妊娠45 d中间、最小体重胎猪和妊娠75 d最大体重胎猪肌酸激酶活性（P<0.05）。与最小体重胎猪相比，高氮能比饲粮组妊娠45和75 d以及低氮能比饲粮组妊娠45和110 d时最大体重胎猪羊水中白蛋白含量显著增加（P<0.05）。随着妊娠时间的延长，2个饲粮组胎猪羊水中尿素和尿酸含量以及尿囊液中尿酸含量逐渐增加。由上可见，高氮能比饲粮可通过调节羊水和尿囊液生化参数促进胎猪的生长发育；与中间和最小体重胎猪相比，最大体重胎猪对蛋白质的利用效率更高。

关键词： 环江香猪; 妊娠时间; 饲粮氮能比; 羊水; 尿囊液; 生化参数

本文引用格式

赵越, 孔祥峰, 姬玉娇, 耿梅梅, 李华伟, 王占彬 . 不同氮能比饲粮对妊娠环江香猪羊水和尿囊液生化参数的影响[J]. 动物营养学报, 2018 , 30(4) : 1574 -1581 . DOI: 10.3969/j.issn.1006-267x.2018.04.043

Abstract

The present study was conducted to investigate the effects of different nitrogen/energy diets on biochemical parameters in amniotic fluid and allantoic fluid of pregnant Huanjiang mini-pigs. Forty-eight primiparous Huanjiang mini-pigs were randomly assigned into one of two groups and reared in 8 pens (replicates) with 3 pigs per pen. After service, the animals in the two groups were fed high nitrogen/energy diet (digestible energy was 14.73 MJ/kg, crude protein content was 13.11%, and nitrogen/energy was 0.89) and low nitrogen/energy diet (digestible energy was 12.24 MJ/kg, crude protein content was 9.77%, and nitrogen/energy was 0.80), respectively. At 45, 75, and 110 days of pregnancy, one pig was selected randomly from each pen and sacrificed. The amniotic fluid and allantoic fluid of fetuses with maximal body weight (BW), middle BW and minimal BW per litter were collected for determining biochemical parameters. The results showed that under the same pregnant time and standard body weight conditions, compared with feeding the low nitrogen/energy diet, feeding the high nitrogen/energy diet significantly increased the alkaline phosphatase (ALP) activity in allantoic fluid of fetuses with minimal BW at 75 days of pregnancy(P<0.05), while significantly decreased the aspartate aminotransferase (AST) activity in allantoic fluid of fetuses with middle or minimal BW at 75 days of pregnancy and fetuses with minimal BW at 110 days of pregnancy, the uric acid (UA) content of fetuses with maximal BW at 45 and 75 days of pregnancy, the creatine kinase (CK) activity of fetuses with middle or minimal BW at 45 days of pregnancy and fetuses with maximal BW at 75 days of pregnancy (P<0.05). Compared with the fetuses with minimal BW, the albumin (ALB) content in amniotic fluid of fetuses with maximal BW at 45 and 75 days of pregnancy in high nitrogen/energy diet group was significantly increased (P<0.05), as well as at 45 and 110 days of pregnancy in low nitrogen/energy diet group (P<0.05). With the pregnant time extension, the urea (UREA) and UA contents in amniotic fluid and UA content in allantoic fluid of fetuses in two diet groups were gradually increased. These findings suggested that the high nitrogen/energy diet can promote the growth and development of fetuses by regulating the biochemical parameters in amniotic fluid and allantoic fluid; the fetuses with maximal BW presents higher utilization efficiency of protein than the fetuses with middle or minimal BW.

Key words： Huanjiang mini-pigs; pregnant time; dietary nitrogen/energy; amniotic fluid; allantoic fluid; biochemical parameters

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