动物营养学报 >

2018 , Vol. 30 >Issue 6: 2143 - 2154

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.06.017

反刍与草食动物营养 Ruminant and herbivore nutrition

补喂5-羟色氨酸、瘤胃保护性5-羟色氨酸对绵羊血浆中5-羟色氨酸、褪黑素含量的影响

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  • 新疆农业大学动物科学学院, 新疆肉乳用草食动物营养重点实验室, 乌鲁木齐 830052
赵芳(1989-),女,新疆阿勒泰人,博士研究生,研究方向为动物营养与代谢。E-mail:937317612@qq.com

收稿日期: 2017-11-22

  网络出版日期: 2018-06-20

基金资助

国家自然科学基金项目（31760681）

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Effects of Diet Supplemented with 5-Hydroxytryptophan, Rumen Protected 5-Hydroxytryptophan on Plasma 5-Hydroxytryptophan and Melatonin Contents of Sheep

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  • Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2017-11-22

  Online published: 2018-06-20

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摘要

本试验旨在研究补喂5-羟色氨酸、瘤胃保护性5-羟色氨酸对绵羊血浆中5-羟色氨酸、褪黑素含量的影响，探究通过5-羟色氨酸调节绵羊机体中褪黑素分泌模式及含量的可能性。试验选取1岁、平均体重为（55.78±3.24）kg的陶赛特母羊18只，按体重分为3组，每组6只，分别为对照组和试验Ⅰ组、试验Ⅱ组，每天每只羊的粉状精料饲喂量为体重的1%，玉米青贮饲喂量为0.6 kg，混合干草自由采食，在此基础上，试验Ⅰ组、试验Ⅱ组羊只分别补喂5-羟色氨酸50 mg/kg BW、瘤胃保护性5-羟色氨酸111 mg/kg BW，进行15 d的饲养试验。结果表明：在早上、下午饲喂后试验组血浆中5-羟色氨酸含量均高于对照组，且在下午饲喂后2个试验组血浆中5-羟色氨酸含量呈波动性上升，但试验Ⅰ组与试验Ⅱ组之间差异不显著（P>0.05）。在早上饲喂后1.5 h，各组血浆中色氨酸含量均呈增加趋势；在下午饲喂后1.5~6.0 h，各组血浆中色氨酸含量呈下降趋势。在白天时，各组血浆中5-羟色胺含量差异不显著（P>0.05）；在下午饲喂后1.5~6.0 h，各组血浆中5-羟色胺含量呈波动性上升。在早上饲喂后3.0~9.0 h，试验Ⅰ组与试验Ⅱ组血浆中褪黑素含量均高于对照组，其中在早上饲喂后6.0 h试验Ⅰ组和试验Ⅱ组与对照组的差异均达到了极显著水平（P<0.01）。在下午饲喂后1.5~9.0 h，各组血浆中褪黑素含量均呈波动性上升，且在下午饲喂后1.5、3.0、6.0 h试验Ⅰ组和试验Ⅱ组极显著高于对照组（P<0.01），在下午饲喂后9.0 h试验Ⅱ组显著高于对照组（P<0.05）。由此可知，补喂5-羟色氨酸（50 mg/kg BW）或瘤胃保护性5-羟色氨酸（111 mg/kg BW）均可显著提高绵羊血浆中5-羟色氨酸含量，改变白天机体中褪黑素分泌模式及含量，且瘤胃保护性5-羟色氨酸的作用更有效，但对血浆中色氨酸、5-羟色胺含量均无显著影响。

关键词： 绵羊; 褪黑素; 5-羟色氨酸; 5-羟色胺

本文引用格式

赵芳, 高超, 王根, 赵国栋, 李晓斌, 马晨, 杨开伦 . 补喂5-羟色氨酸、瘤胃保护性5-羟色氨酸对绵羊血浆中5-羟色氨酸、褪黑素含量的影响[J]. 动物营养学报, 2018 , 30(6) : 2143 -2154 . DOI: 10.3969/j.issn.1006-267x.2018.06.017

Abstract

This experiment aimed to study the effects of diet supplemented with 5-hydroxytryptophan, rumen protected 5-hydroxytryptophan on plasma 5-hydroxytryptophan and melatonin contents of sheep, and to explore the possibility of regulating sheep melatonin secretion mode and content by 5-hydroxytryptophan. Eighteen 1-year-old Dorset sheep with the average body weight of (55.78±3.24) kg were divided into 3 groups according to the body weight, being control group, trial group Ⅰ and trial group Ⅱ. Each sheep in all group was fed with 10% of body weight powder concentrate, 0.6 kg corn silage every day, and had free continuous access to mixed hay, additionally, each sheep in trail group Ⅰ and trial group Ⅱ was fed with 5-hydroxytryptophan (50 mg/kg BW) and rumen protected 5-hydroxytryptophan (111 mg/kg BW), respectively. The supplementary feeding experiment lasted for 15 days. The results showed that plasma 5-hydroxytryptophan content in trial groups were higher than that in control group after feeding in the morning and afternoon, and 5-hydroxytryptophan content in trial groups showed a fluctuating increasing trend after feeding in the afternoon, but the difference between two trial groups was not significant (P>0.05). Plasma tryptophan content in all groups was increased at 1.5 h after feeding in the morning, but it was decreased at 1.5 to 6.0 h after feeding in the afternoon. During the daytime, there was no significant difference in plasma 5-hydroxytryptamine content among all groups (P>0.05). The content of 5-hydroxytryptamine in each group was volatility increased at 1.5 to 6.0 h after feeding in the afternoon. Plasma melatonin content in trail group Ⅰ and trial group Ⅱ was higher than that in control group at 3.0 to 9.0 h after feeding in the morning. Among them, the differences between two trial groups and control groups at 6.0 h after feeding in the morning were significant (P<0.01). Plasma melatonin content in all groups showed a fluctuating increasing trend at 1.5 to 9.0 h after feeding in the afternoon, it in trial groups was significantly higher than that in control group at 1.5, 3.0 and 6.0 h after feeding in the afternoon (P<0.01), and it in trial group Ⅱ was significantly higher than that in control group at 9.0 h after feeding in the afternoon (P<0.05). In conclusion, diet supplemented with 5-hydroxytryptophan (50 mg/kg BW) or rumen protected 5-hydroxytryptophan (111 mg/kg BW) both can significantly elevate sheep plasma 5-hydroxytryptophan content, change melatonin secretion pattern and content in the daytime, and rumen protected 5-hydroxytryptophan shows a better effect, but plasma tryptophan and 5-hydroxytryptamine contents are not be affected significantly.

Key words： sheep; melatonin; 5-hydroxytryptophan; 5-hydroxytryptamine

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