酵母浸粉对家蚕血淋巴中甘油三酯含量和尿酸代谢的影响

李娜, 靳慧杰, 贾漫丽, 黄露, 谢岩, 夏爱华, 杨贵明, 李季生

doi:10.3969/j.issn.1006-267x.2018.06.028

动物营养学报 >

2018 , Vol. 30 >Issue 6: 2245 - 2251

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.06.028

特种经济动物营养 Special economic animal nutrition

酵母浸粉对家蚕血淋巴中甘油三酯含量和尿酸代谢的影响

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1. 河北省高校特产蚕桑应用技术研发中心, 承德 067000;
2. 承德医学院蚕业研究所, 承德 067000;
3. 河北省正定农业综合技术推广站, 石家庄 050800

李娜(1981-),女,河北邢台人,助理研究员,硕士,研究方向为蚕桑生理及饲料。E-mail:liyunxiao2008@163.com

收稿日期: 2017-11-08

网络出版日期: 2018-06-20

基金资助

河北省教育厅重点课题（ZD2016044）

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Effects of Yeast Extract Powder on Triglyceride Content and Uric Acid Metabolism in Hemolymph of Silkworms

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1. Hebei Universities R & D Center for Sericulture and Specialty Enabling Technologies, Chengde 067000, China;
2. Institute of Sericulture, Chengde Medical University, Chengde 067000, China;
3. Zhengding Agro-Tech Extension Station of Hebei, Shijiazhuang 050800, China

Received date: 2017-11-08

Online published: 2018-06-20

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摘要

本试验旨在研究酵母浸粉对家蚕血淋巴中甘油三酯含量和尿酸代谢的影响。以蚕品种"菁松×皓月"为试验材料，挑选大小一致、发育整齐的家蚕600头，随机分成4个组（每组3个重复，每个重复50头，雌雄各占1/2），从5龄起蚕开始，3个试验组（JM1组、JM2组、JM3组）分别饲喂以1%、2%和4%酵母浸粉溶液涂抹的新鲜桑叶，对照组则以蒸馏水替代酵母浸粉溶液，分别在添食酵母浸粉3、4、5 d后检测家蚕血淋巴中尿酸含量、甘油三酯含量以及黄嘌呤氧化酶（XOD）活性。结果表明：添食不同浓度酵母浸粉5 d后家蚕体重增长率均较对照组显著升高（P<0.05），但各试验组之间差异不显著（P>0.05），且随着酵母浸粉浓度的升高，体重增长率呈现下降的趋势。添食不同浓度酵母浸粉5 d后家蚕血淋巴中甘油三酯含量比对照组增加42%~98%，其中JM1组、JM2组与对照组的差异达到显著水平（P<0.05）；随着添食时间的延长，各组家蚕血淋巴中尿酸含量均呈现下降趋势，第5天与第3天相比差异均达到显著水平（P<0.05）；随着酵母浸粉浓度的升高，各时间点家蚕血淋巴中尿酸含量均呈现升高趋势，但各试验组与对照组的差异均未达到显著水平（P>0.05）。3个试验组家蚕血淋巴中XOD活性在各时间点均显著高于对照组（P<0.05），但第3、4天家蚕血淋巴中XOD活性随着酵母浸粉浓度的升高均呈现先上升后下降趋势。家蚕血淋巴中尿酸含量与XOD活性之间存在显著或极显著的正相关性（P<0.05或P<0.01），相关系数在0.652~0.902。由此得出，添食一定浓度的酵母浸粉可导致家蚕血淋巴中尿酸含量、甘油三酯含量以及黄嘌呤氧化酶（xanthine oxidase，XOD）活性升高，从而影响家蚕的尿酸代谢。

关键词： 家蚕; 酵母浸粉; 甘油三酯; 尿酸代谢

本文引用格式

李娜, 靳慧杰, 贾漫丽, 黄露, 谢岩, 夏爱华, 杨贵明, 李季生 . 酵母浸粉对家蚕血淋巴中甘油三酯含量和尿酸代谢的影响[J]. 动物营养学报, 2018 , 30(6) : 2245 -2251 . DOI: 10.3969/j.issn.1006-267x.2018.06.028

Abstract

This experiment was conducted to investigate the effects of yeast extract powder (YEP) on the content of triglyceride and the metabolism of uric acid (UA) in hemolymph of silkworms (Bombyx mori). The silkworm variety "Jingsong×Haoyue" was used as experimental material. Choosing similar size and development of 600 silkworms randomly divided into four groups with each group had three replicates and each replicate had 50 silkworms (half male and half female). Fifth instar larvae of silkworms in experimental groups (groups JM1, JM2 and JM3) were fed fresh mulberry leaves painted with 1%, 2% and 4% YEP solution,respectively, and those in control group were fed fresh mulberry leaves painted with distilled water. The UA content, triglyceride (TG) content and xanthine oxidase (XOD) activity in hemolymph of silkworms were detected after feeding with different concentrations of YEP for 3, 4 and 5 days. The results showed that the body weight growth rate of silkworms increased significantly after feeding with different concentrations of YEP for 5 days compared with the control group (P<0.05), but no significant differences were found among experimental groups (P>0.05), and with the increase of YEP concentration, the body weight growth rate showed a trend of decline. The content of TG in hemolymph of silkworms after feeding with different concentrations of YEP for 5 days was 1.42 to 1.98 times higher than that of control group, and the differences between the control group and the groups JM1 or JM2 were significant (P<0.05). The content of UA in hemolymph of silkworms of each group showed a trend of decline with the extension of time. Compared with the 3rd day, the content of UA in hemolymph of silkworms of each group was significantly decreased compared with the 5th day (P<0.05). The content of UA in hemolymph of silkworms of each time point showed a trend of increase with the increase of YEP concentration, but the differences were not significant between the control group and the experimental groups (P>0.05). The activity of XOD in hemolymph of silkworms of each time point was significantly higher than that of control group (P<0.05), but it was firstly increased and then decreased with the increase of YEP concentration. There was a significant or an extremely significant correlation between the content of UA and the activity of XOD in hemolymph of silkworms (P<0.05 or P<0.01), and the correlation coefficient ranged from 0.652 to 0.902. It is concluded that feeding a certain concentration of YEP can lead to increases of UA content, TG content and XOD activity in hemolymph, thus affecting the UA metabolism of silkworms.

Key words： silkworms; yeast extract powder; triglyceride; uric acid metabolism

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