分子营养 Molecular Nutrition

植物乳杆菌对大肠杆菌感染猪肠道上皮细胞形态、存活和免疫应答的影响

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  • 北京市农林科学院畜牧兽医研究所, 北京 100097
曾燕霞(1990-),女,甘肃榆中人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:1197255876@qq.com

收稿日期: 2017-11-20

  网络出版日期: 2018-06-20

基金资助

北京市农林科学院青年基金(QNJJ201607);生猪产业技术体系北京市创新团队(BAIC02—2017);北京市农林科学院创新能力建设专项(KJCX20180109)

Effects of Lactobacillus plantarum on Morphological, Survival and Immunity Response of Escherichia coli Infected Intestinal Epithelial Cells

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  • Institute of Animal Husbandry and Veterinary Medicine, Beijing 100097, China

Received date: 2017-11-20

  Online published: 2018-06-20

摘要

本试验旨在探讨植物乳杆菌对大肠杆菌感染猪肠道上皮细胞(IPEC-J2细胞)形态、存活和免疫应答的影响。在体外条件下,采用吉姆萨和台盼蓝染色方法检测植物乳杆菌对大肠杆菌感染IPEC-J2细胞形态和存活的影响,并采用实时定量荧光PCR方法研究了其对大肠杆菌感染IPEC-J2细胞免疫应答的影响。结果表明:植物乳杆菌能够缓解大肠杆菌引起的IPEC-J2细胞形态损伤。与大肠杆菌处理组相比,植物乳杆菌与大肠杆菌共同处理可显著或极显著降低细胞死亡率(1、2 h,P<0.01;3 h,P<0.05)。植物乳杆菌可显著或极显著抑制大肠杆菌引起的IPEC-J2细胞Toll样模式识别受体2(TLR2)(2、3 h,P<0.01)、Toll样模式识别受体6(TLR6)(2 h,P<0.05;3 h,P<0.01)、NOD样模式识别受体2(NOD2)(1 h,P<0.05;2 h,P<0.01)及炎症因子白细胞介素-6(IL-6)(2、3 h,P<0.01)、白细胞介素-8(IL-8)(2、3 h,P<0.01)mRNA的过表达(P<0.05),还可显著或极显著促进IPEC-J2细胞NOD样模式识别受体1(NOD1)mRNA的表达(2 h,P<0.05;3 h,P<0.01)。结果提示,植物乳杆菌能够缓解大肠杆菌引起的IPEC-J2细胞形态损伤及死亡,并可通过调节模式识别受体TLR2、TLR6和NOD2 mRNA的表达缓解大肠杆菌引起的细胞炎症因子IL-6、IL-8 mRNA的过表达。

本文引用格式

曾燕霞, 王四新, 张伟, 刘辉, 张董燕, 王雅民, 季海峰, 王晶 . 植物乳杆菌对大肠杆菌感染猪肠道上皮细胞形态、存活和免疫应答的影响[J]. 动物营养学报, 2018 , 30(6) : 2262 -2270 . DOI: 10.3969/j.issn.1006-267x.2018.06.030

Abstract

The aim of this study was to investigate the effects of Lactobacillus plantarum on morphological, survival and immunity response of Escherichia coli infected intestinal epithelial cells (IPEC-J2 cells). Under the conditions of in vitro, the effects of Lactobacillus plantarum on morphological and survival of Escherichia coli infected IPEC-J2 cells were assessed using giemsa staining and trypan blue staining methods, and the effects of Lactobacillus plantarum on immunity response of Escherichia coli infected IPEC-J2 cells were assessed using real-time PCR method. The results showed that Lactobacillus plantarum could ameliorate Escherichia coli-induced disruption of cell morphology on IPEC-J2 cells. Compared with the Escherichia coli treated group, the perecentage of dead cells was significantly decreased by Lactobacillus plantarum and Escherichia coli treated (1, 2 h, P<0.01; 3 h, P<0.05). The Lactobacillus plantarum could significantly inhibit the mRNA overexpressions of Toll-like receptor 2 (TLR2) (2, 3 h,P<0.01), Toll-like receptor 6 (TLR6) (2 h,P<0.05;3 h,P<0.01), NOD-like receptor 2 (NOD2) (1 h,P<0.05;2 h,P<0.01) and inflammatory cytokines interleukin-6 (IL-6) (2, 3 h,P<0.01), interleukin-8 (IL-8) (2, 3 h,P<0.01) in IPEC-J2 cells induced by Escherichia coli, and could promote the NOD-like receptor 1 (NOD1) mRNA expression in IPEC-J2 cells (2 h,P<0.05;3 h,P<0.01). The results indicated that Lactobacillus plantarum can ameliorate Escherichia coli-induced the disruption of cell morphology and death of IPEC-J2 cells. In addition, it can suppress the Escherichia coli-induced mRNA overexpressions of inflammatory cytokines IL-6 and IL-8 by regulating the pattern recognition receptor mRNA expression of TLR2, TLR6 and NOD2.

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