动物营养学报 >

2018 , Vol. 30 >Issue 6: 2337 - 2345

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.06.038

饲料营养 Feed science and technology

抗菌肽Sublancin与黄芪多糖对小鼠免疫调节作用的比较研究

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  • 1. 中国农业大学动物科技学院, 生物饲料添加剂北京市重点实验室, 北京 100193;
    2. 华中农业大学动物科技学院, 武汉 430070
杨天任(1993-),男,山东济南人,硕士研究生,动物营养与饲料科学专业。E-mail:25190557@qq.com

收稿日期: 2017-12-01

  网络出版日期: 2018-06-20

基金资助

农业产业技术体系北京市生猪创新团队项目；北京市科技计划项目

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Comparative Study on Immunomodulatory Effect of Antimicrobial Peptide Sublancin and Astragalus Polysaccharide of Mice

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  • 1. Beijing Key Laboratory of Biological Feed Additive, China Agricultural University, Beijing 100193, China;
    2. College of Animal Sciences & Technology, Huazhong Agricultural University, Wuhan 430070, China

Received date: 2017-12-01

  Online published: 2018-06-20

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摘要

本试验旨在比较抗菌肽Sublancin与黄芪多糖对小鼠免疫功能的调节作用。选用4~6周龄健康雌性BALB/c小鼠60只，随机分成6组，每组10只。各组小鼠先连续灌胃7 d、每天1次、每只0.2 mL的下列物质：空白对照组，灌胃生理盐水；攻毒组，灌胃生理盐水；12.0 mg/kg BW黄芪多糖组，灌胃12.0 mg/kg BW的黄芪多糖溶液；48.0 mg/kg BW黄芪多糖组，灌胃48.0 mg/kg BW的黄芪多糖溶液；1.0 mg/kg BW Sublancin组，灌胃1.0 mg/kg BW的Sublancin溶液；2.0 mg/kg BW Sublancin组，灌胃2.0 mg/kg BW的Sublancin溶液。除空白对照组外，其余5组在灌胃结束24 h后均以200 μL/只的剂量灌胃浓度为1×109 CFU/mL的鼠伤寒沙门氏菌进行攻毒。分别在攻毒3和24 h后从每组随机取5只小鼠采集外周血、脾脏以及盲肠内容物，检测血清中细胞因子含量，脾细胞中T淋巴细胞亚群CD4+、CD8+数量以及肠道内容物中沙门氏菌数量等指标。结果表明：沙门氏菌攻毒3 h后，与攻毒组相比，12.0和48.0 mg/kg BW黄芪多糖组以及2.0 mg/kg BW Sublancin组血清中白细胞介素-6（IL-6）的含量均显著降低（P<0.05）；1.0 mg/kg BW Sublancin组血清中白细胞介素-10（IL-10）的含量显著提高（P<0.05）；12.0和48.0 mg/kg BW黄芪多糖组以及1.0和2.0 mg/kg BW Sublancin组血清中肿瘤坏死因子-α（TNF-α）的含量均显著降低（P<0.05），但血清中白细胞介素-25（IL-25）的含量无显著变化（P>0.05）；48.0 mg/kg BW黄芪多糖组和2.0 mg/kg BW Sublancin组血清中单核细胞趋化蛋白-1（MCP-1）的含量均显著降低（P<0.05）；48.0 mg/kg BW黄芪多糖组以及1.0和2.0 mg/kg BW Sublancin组脾细胞中CD4+/CD8+均显著提高（P<0.05）；12.0和48.0 mg/kg BW黄芪多糖组以及1.0和2.0 mg/kg BW Sublancin组肠道内容物中沙门氏菌的数量均没有产生显著变化（P>0.05），但是存在降低趋势。沙门氏菌攻毒24 h后，与攻毒组相比，2.0 mg/kg BW Sublancin组血清中IL-6的含量显著降低（P<0.05）；48.0 mg/kg BW黄芪多糖组和2.0 mg/kg BW Sublancin组血清中TNF-α的含量显著降低（P<0.05）；12.0和48.0 mg/kg BW黄芪多糖组以及2.0 mg/kg BW Sublancin组血清中MCP-1的含量显著降低（P<0.05）。此外，沙门氏菌攻毒24 h后，与空白对照组相比，2.0和48.0 mg/kg BW黄芪多糖组以及1.0和2.0 mg/kg BW Sublancin组血清中IL-10的含量均显著提高（P<0.05）。综合上述结果可得出，适宜剂量的抗菌肽Sublancin和黄芪多糖对小鼠免疫功能均有良好的调节作用；与黄芪多糖相比，抗菌肽Sublancin对感染沙门氏菌小鼠免疫功能的调节更加全面。

关键词： 抗菌肽; Sublancin; 黄芪多糖; 小鼠; 免疫功能

本文引用格式

杨天任, 王帅, 黄烁, 尚丽君, 于海涛, 曾祥芳, 谯仕彦 . 抗菌肽Sublancin与黄芪多糖对小鼠免疫调节作用的比较研究[J]. 动物营养学报, 2018 , 30(6) : 2337 -2345 . DOI: 10.3969/j.issn.1006-267x.2018.06.038

Abstract

This experiment was conducted to compare the regulating effect of antimicrobial peptide Sublancin and Astragalus polysaccharide on immune function of mice. Sixty female BALB/c mice at the age of 4 to 6 weeks were randomly assigned into 6 groups with 10 mice per group. Mice in blank control group received saline by intragastric administration, mice in challenged group received saline by intragastric administration, mice in 12.0 and 48.0 mg/kg BW Astragalus polysaccharide groups received 12.0 and 48.0 mg/kg BW Astragalus polysaccharide solution by intragastric administration, respectively, and mice in 1.0 and 2.0 mg/kg BW Sublancin groups received 1.0 and 2.0 mg/kg BW Sublancin solution by intragastric administration, respectively. All the mice were administrated orally daily for 7 days, and the dose of each mouse was 0.2 mL. Except blank control group, mice in other 5 groups were challenged with Salmonella typhimurium (1×109 CFU/mL, 200 μL per mouse) on day 8 (24 h after intragastric administration). Five mice from each group were randomly selected 3 and 24 h after challenge and the peripheral blood, spleen and caecum contents were sampled to measure the serum cytokine contents, T lymphocyte subsets CD4+ and CD8+ numbers in splenocyte and Salmonella number in intestinal contents, and so on. The results showed as follows:challenged 3 h by Salmonella, compared with the challenged group, the serum interleukin-6 (IL-6) content in 12.0 and 48.0 mg/kg BW Astragalus polysaccharide groups and 2.0 mg/kg BW Sublancin group was significantly reduced (P<0.05); the serum interleukin-10 (IL-10) content in 1.0 mg/kg BW Sublancin group was significantly increased (P<0.05); the serum tumor necrosis factor-α (TNF-α) content in 12.0 and 48.0 mg/kg BW Astragalus polysaccharide groups, 1.0 and 2.0 mg/kg BW Sublancin groups was significantly reduced (P<0.05), but the serum interleukin-25 (IL-25) content was not significantly different (P>0.05); the serum monocyte chemoattractant protein-1 (MCP-1) content in 48.0 mg/kg BW Astragalus polysaccharide group and 2.0 mg/kg BW Sublancin group was significantly reduced (P<0.05); CD4+/CD8+ in splenocyte in 48.0 mg/kg BW Astragalus polysaccharide group, 1.0 and 2.0 mg/kg BW Sublancin groups was significantly increased (P<0.05); the Salmonella number in intestinal contents in 12.0 and 48.0 mg/kg BW Astragalus polysaccharide groups, 1.0 and 2.0 mg/kg BW Sublancin groups had no significant changes (P>0.05), but had reducing trend. Challenged 24 h by Salmonella, compared with the challenged group, the serum IL-6 content in 2.0 mg/kg BW Sublancin group was significantly reduced (P<0.05); the serum TNF-α content in 48.0 mg/kg BW Astragalus polysaccharide group and 2.0 mg/kg BW Sublancin group was significantly reduced (P<0.05); the serum MCP-1 content in 12.0 and 48.0 mg/kg BW Astragalus polysaccharide groups, 2.0 mg/kg BW Sublancin group was significantly reduced (P<0.05). Moreover, the serum IL-10 content in 12.0 and 48.0 mg/kg BW Astragalus polysaccharide groups, 1.0 and 2.0 mg/kg BW Sublancin groups was significantly increased challenged 24 h by Salmonella compared with blank control group (P<0.05). These results indicate that antimicrobial peptide Sublancin and Astragalus polysaccharide have regulating effect on immune function for mice, and the immunomodulatory effect of antimicrobial peptide Sublancin is more comprehensive than that of Astragalus polysaccharide.

Key words： antimicrobial peptide; Sublancin; Astragalus polysaccharide; mice; immune function

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