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苘麻叶总鞣质的提取工艺及抗氧化活性研究

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  • 沈阳农业大学畜牧兽医学院, 沈阳 110866
杨杰(1993-),女,河北沧州人,硕士研究生,研究方向为兽医药理与毒理。E-mail:15802443206@163.com

收稿日期: 2017-11-28

  网络出版日期: 2018-06-20

基金资助

国家自然科学基金(31402252);沈阳农业大学青年基金(20131001)

Research in Extraction Process and Antioxidant Activity of Total Tannin from Abutilon theophrasti Medic. Leaves

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  • College of Animal Husbandry and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China

Received date: 2017-11-28

  Online published: 2018-06-20

摘要

为研究苘麻叶总鞣质的提取工艺及抗氧化活性,本研究通过单因素试验和响应面试验优化苘麻叶总鞣质的提取工艺,同时采用1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除法、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基清除法和铁离子还原/抗氧化能力(FRAP)测定法等评价苘麻叶总多酚、不被吸附的多酚和总鞣质的体外抗氧化活性。结果表明,苘麻叶总鞣质的最佳提取工艺为:丙酮浓度70%、液料比15.25 mL/g、提取时间20 min;在此工艺条件下,8月份、9月份和10月份采收的苘麻叶总鞣质的含量分别为(1.41±0.05)‰、(1.77±0.03)‰和(1.49±0.03)‰;苘麻叶总鞣质具有较好的体外抗氧化活性。本研究得出了苘麻叶总鞣质的高效提取工艺,且提取的总鞣质具有较好的抗氧化活性。

本文引用格式

杨杰, 陈晓云, 郭玉茹, 高翔, 田春莲, 刘明春 . 苘麻叶总鞣质的提取工艺及抗氧化活性研究[J]. 动物营养学报, 2018 , 30(6) : 2377 -2384 . DOI: 10.3969/j.issn.1006-267x.2018.06.043

Abstract

In order to investigate extraction process and antioxidant activity of total tannins from Abutilon theophrasti Medic. leaves, single factor experiments and response surface experiments were established to optimize the extraction process of total tannins from Abutilon theophrasti Medic. leaves, and antioxidant activities of total polyphenols, non-absorbed polyphenols and total tannins were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS)free radical scavenging assay and Ferric ion reducing antioxidant power (FRAP) analysis. The results showed that the optimal extraction conditions were as follows:acetone concentration was 70%, the ratio of solvent to material was 15.25 mL/g and the extraction time was 20 min. Under the above conditions, the yield of total tannins from Abutilon theophrasti Medic. leaves collected in August, September, October was (1.41±0.05)‰, (1.77±0.03)‰ and (1.49±0.03)‰, respectively. Total tannins extracted from Abutilon theophrasti Medic. leaves showed good antioxidant activity. In conclusion, extraction processes with high extraction rate for Abutilon theophrasti Medic. leaves are obtained in the present study, and the extracted total tannins have high antioxidant activity.

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