动物营养学报 >

2018 , Vol. 30 >Issue 7: 2519 - 2528

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.07.012

猪营养 Swine Nutrition

饲粮中添加表皮生长因子对断奶仔猪血清生化指标、血清游离氨基酸和小肠黏膜水解氨基酸含量的影响

展开
  • 1. 湖南农业大学动物医学院, 长沙 410128;
    2. 中国科学院亚热带农业生态研究所, 中国科学院亚热带农业生态过程重点实验室, 长沙 410125;
    3. 湖南师范大学生命科学学院, 动物营养与人体健康实验室, 长沙 410081
朱繁(1992-),男,湖南湘潭人,硕士研究生,研究方向为动物营养与健康养殖。E-mail:976525013@qq.com

收稿日期: 2018-01-03

  网络出版日期: 2018-07-20

基金资助

国家自然科学基金（31402089，31330075）

收起

Effects of Dietary Epidermal Growth Factor on Serum Biochemical Indices, Serum Free Amino Acid and Intestinal Mucosal Hydrolytic Amino Acid Contents of Weaned Piglets

Expand
  • 1. Animal Medical School of Hunan Agricultural University, Changsha 410128, China;
    2. Key Laboratory of Subtropical Agricultural Ecological Process of Chinese Academy of Sciences, Sub-Tropical Agricultural Ecology Research Institute of Chinese Academy of Sciences, Changsha 410125, China;
    3. Animal Nutrition And Human Health Laboratory, Life Sciences College, Hunan Normal University, Changsha 410081, China

Received date: 2018-01-03

  Online published: 2018-07-20

Fold

摘要

本试验旨在研究饲粮中添加表皮生长因子（EGF）对断奶仔猪血清生化指标、血清游离氨基酸和小肠黏膜水解氨基酸含量的影响。选取体重相近、健康的21日龄断奶仔猪42头，随机分成3组，每组14个重复，每个重复1头仔猪。Ⅰ组为对照组，饲喂基础饲粮；Ⅱ组和Ⅲ组分别饲喂在基础饲粮中添加200和400 μg/kg EGF的试验饲粮。试验期为14 d。结果表明：1）饲粮中添加EGF对断奶仔猪平均日增重、平均日采食量和料重比没有显著影响（P>0.05）。2）断奶后7 d，Ⅱ组血清尿酸含量显著高于Ⅰ组（P<0.05），而Ⅱ组和Ⅲ组血清免疫球蛋白A含量显著低于Ⅰ组（P<0.05）。断奶后14 d，Ⅲ组血清肌酐含量显著低于Ⅰ组（P<0.05），且Ⅲ组血清谷草转氨酶/谷丙转氨酶值显著低于Ⅰ组和Ⅱ组（P<0.05）。饲粮中添加EGF对断奶仔猪血清尿素氮、葡萄糖、总蛋白、白蛋白、球蛋白、免疫球蛋白M、免疫球蛋白G、胰岛素样生长因子-1、生长激素含量，白蛋白/球蛋白，谷草转氨酶、谷丙转氨酶活性没有显著影响（P>0.05）。3）断奶后7 d，Ⅱ组血清牛磺酸、丙氨酸、瓜氨酸和组氨酸含量显著低于Ⅲ组（P<0.05），Ⅱ组血清精氨酸、谷氨酸和丝氨酸含量显著低于Ⅰ组和Ⅲ组（P<0.05），各组之间其他血清氨基酸含量无显著差异（P>0.05）。4）断奶后14 d，与Ⅰ组和Ⅲ组比，Ⅱ组空肠黏膜缬氨酸和回肠黏膜半胱氨酸含量显著升高（P<0.05），Ⅱ组空肠黏膜丝氨酸、谷氨酸、丙氨酸、半胱氨酸、异亮氨酸、苯丙氨酸和组氨酸含量也有升高趋势（P<0.10）。综上所述，饲粮中添加高剂量EGF能够降低断奶仔猪血清免疫球蛋白A等生化指标含量，而饲粮中添加低剂量EGF能够降低血清和小肠黏膜部分氨基酸含量。

关键词： EGF; 断奶仔猪; 血清生化指标; 氨基酸; 肠道

本文引用格式

朱繁, 王丽霞, 贾杏林, 尹佳, 杨焕胜, 印遇龙 . 饲粮中添加表皮生长因子对断奶仔猪血清生化指标、血清游离氨基酸和小肠黏膜水解氨基酸含量的影响[J]. 动物营养学报, 2018 , 30(7) : 2519 -2528 . DOI: 10.3969/j.issn.1006-267x.2018.07.012

Abstract

The objective of this experiment was to investigate the effects of dietary epidermal growth factor (EGF) on serum biochemical index, serum free amino acid and intestinal mucosal hydrolytic amino acid contents of weaned piglets. Forty-two healthy piglets with similar body weight were weaned at 21 days of age and randomly assigned into 3 groups with 14 replicates per group and 1 piglet per replicate. Piglets in group Ⅰ (control group) were fed a basal diet, and the others in groups Ⅱ and Ⅲ were fed the basal diet supplemented with 200 and 400 μg/kg EGF, respectively. The experiment lasted for 14 days. The results showed as follows:1) dietary supplemented with EGF had no significant effects on average daily gain, average daily feed intake and feed to gain ratio of weaned piglets (P>0.05). 2) After weaning 7 days, the serum uric acid content in group Ⅱ was significantly higher than that in group Ⅰ (P<0.05), the serum immunoglobulin A content in groups Ⅱ and Ⅲ was significantly lower than that in group Ⅰ (P<0.05). After weaning 14 days, the serum creatinine content in group Ⅲ was significantly lower than that in group Ⅰ (P<0.05), the serum glutamic-oxalacetic transaminase/glutamic-pyruvic transaminase value in group Ⅲ was significantly lower than that in groups Ⅰ and Ⅱ (P<0.05). Dietary supplemented with EGF had no significant effects on serum urea nitrogen, glucose, total protein, albumin, globulin, immunoglobulin M, immunoglobulin G, insulin-like growth factor-1, growth hormone contents, and albumin/globulin and glutamic-oxalacetic transaminase and glutamic-pyruvic transaminase activities of weaned piglets (P>0.05). 3) After weaning 7 days, the contents of taurine, alanine, citrullinine and histidine in serum in group Ⅱ were significantly lower than those in group Ⅲ (P<0.05), the contents of arginine, glutamate and serine in serum in group Ⅱ were significantly lower than those in groups Ⅰ and Ⅲ (P<0.05), and there were no significant differences on other serum amino acid contents among all groups (P>0.05). 4) After weaning 14 days, compared with groups Ⅰand Ⅲ, the contents of valine in jejunum mucosa and cysteine in ileum mucosa in group Ⅱ were significantly increased (P<0.05), and the contents of serine, glutamic acid, alanine, cysteine, isoleucine, phenylalanine and histidine in jejunum mucosa in group Ⅱ were tended to be decreased (P<0.10). In conclusion, dietary supplemented with high dose EGF can reduce serum immunoglobulin A and other biochemical indices contents, while dietary supplemented with low dose EGF can reduce the contents of some amino acid in serum and intestinal mucosa.

Key words： EGF; weaned piglets; serum biochemical indices; amino acids; intestine

参考文献

[1] KONG X F,WU G Y,LIAO Y P,et al.Effects of Chinese herbal ultra-fine powder as a dietary additive on growth performance,serum metabolites and intestinal health in early-weaned piglets[J].Livestock Science,2007,108(1/2/3):272-275.

[2] MAIN R G,DRITZ S S,TOKACH M D,et al.Increasing weaning age improves pig performance in a multisite production system[J].Journal of Animal Science,2004,82(5):1499-1507.  

[3] ZHONG J F,WU W G,ZHANG X Q,et al.Effects of dietary addition of heat-killed Mycobacterium phlei on growth performance,immune status and anti-oxidative capacity in early weaned piglets[J].Archives of Animal Nutrition,2016,70(4):249-262.  

[4] GU X H,LI D F,SHE R P.Effect of weaning on small intestinal structure and function in the piglet[J].Archiv Für Tierernahrung,2002,56(4):275-286.  

[5] 李垚,单安山,镡龙,等.表皮生长因子和胰岛素样生长因子-Ⅰ对断奶仔猪免疫功能的影响[J].中国畜牧杂志,2006,42(3):17-20.

[6] 胡莹莹,王琦,陈瑛.表皮生长因子及其作用机制[J].哈尔滨师范大学(自然科学版),2013,29(3):91-95.

[7] BEDFORD A.The effect of Lactococcus lactis-produced epidermal growth factor on the growth and intestine development of early-weaned pigs[D].Ph.D.Thesis.Ontario:The University of Guelph,2014:65-95.

[8] CLARK J A,GAN H,SAMOCHA A J,et al.Enterocyte-specific epidermal growth factor prevents barrier dysfunction and improves mortality in murine peritonitis[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2009,297(3):G471-G479.

[9] CHEUNG Q C,YUAN Z F,DYCE P W,et al.Generation of epidermal growth factor-expressing Lactococcus lactis and its enhancement on intestinal development and growth of early-weaned mice[J].American Journal of Clinical Nutrition,2009,89(3):871-879.  

[10] WANG S J,ZHOU L,CHEN H N,et al.Analysis of the biological activities of Saccharomyces cerevisiae expressing intracellular EGF,extracellular EGF,andtagged EGF in early-weaned rats[J].Applied Microbiology and Biotechnology,2015,99(5):2179-2189.  

[11] CELLINI C,XU J,BUCHMILLER-CRAIR T.Effect of epidermal growth factor on small intestinal sodium/glucose cotransporter-1 expression in a rabbit model of intrauterine growth retardation[J].Journal of Pediatric Surgery,2005,40(12):1892-1897.  

[12] KANG P,TOMS D,YIN Y L,et al.Epidermal growth factor-expressing Lactococcus lactis enhances intestinal development of early-weaned pigs[J].The Journal of Nutrition,2010,140(4):806-811.  

[13] WANG S J,GUO C H,ZHOU L,et al.Comparison of the biological activities of Saccharomyces cerevisiae-expressed intracellular EGF,extracellular EGF,and tagged EGF in early-weaned pigs[J].Applied Microbiology and Biotechnology,2015,99(17):7125-7135.  

[14] LEE D N,KUO T Y,CHEN M C,et al.Expression of porcine epidermal growth factor in Pichia pastoris and its biology activity in early-weaned piglets[J].Life Sciences,2006,78(6):649-654.  

[15] CELLINI C J,XU A,ARRIAGA A,et al.Effect of epidermal growth factor infusion on fetal rabbit intrauterine growth retardation and small intestinal development[J].Journal of Pediatric Surgery,2004,39(6):891-897.  

[16] BEDFORD A,HUYNH E,FU M L,et al.Growth performance of early-weaned pigs is enhanced by feeding epidermal growth factor-expressing Lactococcus lactis fermentation product[J].Journal of Biotechnology,2014,173:47-52.

[17] BEDFORD A,LI Z,LI M,et al.Epidermal growth factor-expressing Lactococcus lactis enhances growth performance of early-weaned pigs fed diets devoid of blood plasma[J].Journal of Animal Science,2012,90(Suppl.4):4-6.

[18] LEE D N,CHANG W F,YU I T,et al.Effects of diets supplemented with recombinant epidermal growth factor and glutamine on gastrointestinal tract development of early-weaned piglets[J].Asian-Australasian Journal of Animal Sciences,2008,21(4):582-589.  

[19] BEDFORD A,CHEN T,HUYNH E,et al.Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo:potential mechanisms involved[J].Journal of Biotechnology,2015,196-197:9-19.

[20] WANG J P,YOO J S,KIM H J,et al.Nutrient digestibility,blood profiles and fecal microbiota are influenced by chitooligosaccharide supplementation of growing pigs[J].Livestock Science,2009,125(2/3):298-303.

[21] 平凡,晏婷婷,汪悦.尿酸在肠道代谢机制中的研究进展[J].医学研究生学报,2015,28(7):763-766.

[22] 杨俊伟,黎磊石,许瑞吉.糖尿病大鼠的肾脏肥大和尿EGF变化[J].肾脏病与透析肾移植杂志,1993,2(3):235-238.

[23] 姚军虎,王康宁,宋代军.肉仔鸡血浆游离氨基酸浓度变化规律的研究[J].西北农业大学学报,1999,27(6):97-101.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2