分子营养 Molecular Nutrition

热休克蛋白70在牦牛胃中的分布和表达

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  • 甘肃农业大学动物医学院, 兰州 730070
杨晓晴(1990-),女,甘肃天水人,硕士研究生,从事动物组织学与胚胎学研究。E-mail:1453685951@qq.com

收稿日期: 2017-12-19

  网络出版日期: 2018-07-20

基金资助

国家自然科学基金(31572478)

Distribution and Expression of Heat Shock Protein 70 in Stomachs of Yaks

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  • College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2017-12-19

  Online published: 2018-07-20

摘要

本试验旨在研究热休克蛋白70(HSP70)在牦牛胃中的分布特点和表达量的差异性变化。采集8头健康成年牦牛的胃组织,采用组织学染色方法研究皱胃腺体间细胞的类型,采用免疫组化法和蛋白质免疫印迹(WB)法分别检测HSP70在不同胃的分布及表达量。结果表明:嗜银染色及阿利新蓝-过碘酸-雪夫(AB-PAS)染色发现,皱胃腺体间含有大量的内分泌细胞。免疫组化检测发现,在牦牛瘤胃、网胃、瓣胃,HSP70的阳性表达仅见于黏膜上皮细胞层,角质层细胞呈强阳性表达,而基底层、颗粒层和棘层的部分细胞呈中等阳性表达,部分细胞未见阳性表达;皱胃阳性反应的范围较广,不仅黏膜表面上皮细胞有表达,胃腺间的内分泌细胞及壁细胞也呈阳性表达。且阳性反应主要集中在细胞的胞质和胞核。免疫组化和WB结果均表明,HSP70在牦牛皱胃表达量最高,瘤胃、网胃及瓣胃依次递减,部位间差异极显著(P<0.01)。综合得出,牦牛皱胃腺体间含有大量嗜银性内分泌细胞,HSP70在所有胃中均有分布,且在皱胃的表达量显著高于其他胃,提示HSP70可能与牦牛胃的消化吸收有一定的关联性。

本文引用格式

杨晓晴, 崔燕, 何俊峰, 杨雪, 刘鹏刚, 廖博, 李慧, 孙娟 . 热休克蛋白70在牦牛胃中的分布和表达[J]. 动物营养学报, 2018 , 30(7) : 2626 -2631 . DOI: 10.3969/j.issn.1006-267x.2018.07.022

Abstract

The purpose of this study was to study the distribution characteristics and the differential changes of expression level of heat shock protein 70 (HSP70) in stomachs of yaks. The tissues of stomachs of 8 adult healthy yaks were collected, and the type of cells in the abomasal gland was studied by histological staining. The distribution and expression of HSP70 in different stomachs were detected by the methods of immunohistochemistry and Western blotting, respectively. The results showed as follows:Gordon sweet staining and Alcian blue-periodic acid-Schiff (AB-PAS) staining showed that a large number of endocrine cells were found in the abomasal gland. Immunohistochemistry analysis showed that in rumen, reticulum and omasum, HSP70 only positively expressed in the epithelial cell layer, strongly positively expressed in stratum corneum cells, some cells in basal layer, granular layer and spinous layer were moderately positive expressed, whereas some other cells were negatively expressed. The scope of the abomasum positive reaction was very wide, not only expressed in the epithelial cells of mucous surface, but also expressed in the endocrine cells and parietal cells in the abomasal gland. Meanwhile, the positive reaction was mainly concentrated in the cytoplasm and nucleus. Immunohistochemistry and Western blotting results both showed that the highest expression level of HSP70 was found in the abomasal gland of yaks, the expression level in rumen, reticulum and omasum was descended in order, and the differences among parts were significant (P<0.01). In conclusion, a large number of argyrophilia endocrine cells are found in the abomasal gland of yaks. HSP70 is distributed in all stomachs of yaks, and the expression of the abomasum is obviously higher than that of other stomachs, which suggests that HSP70 may be associated with digestion and absorption of yaks' stomach.

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