动物营养学报 >

2018 , Vol. 30 >Issue 7: 2652 - 2661

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.07.025

饲料营养 Feed science and technology

壳寡糖对氧化应激仔猪生长性能、抗氧化能力及空肠养分消化和转运能力的影响

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  • 四川农业大学动物营养研究所, 动物抗病营养教育部重点实验室, 成都 611130
田刚(1974-),男,重庆黔江人,副教授,博士,从事单胃动物营养研究。E-mail:tgang2008@126.com

收稿日期: 2017-12-13

  网络出版日期: 2018-07-20

基金资助

国家自然科学基金项目（31372324）；四川省科技支撑计划项目（2016NZ006）

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Effects of Chitooligosaccharides on Growth Performance, Antioxidant Capacity and Nutrient Digestion and Transport Capacity of Jejunum of Oxidative Stress Piglets

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  • 1. Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2017-12-13

  Online published: 2018-07-20

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摘要

本试验旨在研究饲粮添加壳寡糖（COS）对正常饲养和氧化应激仔猪生长性能、抗氧化能力及空肠养分消化和转运能力的影响。选择24日龄、平均体重（7.34±0.09）kg的健康"杜×长×大"断奶仔猪24头，按照2×2双因子试验设计，随机分为对照组、COS组、敌草快（diquat）组和COS+diquat组，每组6个重复，每个重复1头仔猪。试验期28 d。饲粮COS添加量为50 mg/kg，饲喂贯穿试验全程，于试验第22天进行一次性腹腔注射10 mg/kg BW diquat，不注射diquat试验猪腹腔注射等量生理盐水。于试验第18~21天采用内源指示剂法进行消化试验，第22天早上试验猪空腹前腔静脉采血后再进行diquat处理，第29天早上试验猪前腔静脉采血后屠宰取空肠黏膜样品待测。结果表明：1）注射diquat前，饲粮添加COS对仔猪的平均日增重（ADG）和平均日采食量（ADFI）无显著影响（P>0.05），但有降低料重比（F/G）的趋势（P=0.09）；显著升高仔猪对饲粮干物质、有机物、粗蛋白质、粗脂肪、能量、粗灰分、钙和磷的表观消化率（P<0.05）；显著升高血浆超氧化物歧化酶（SOD）活性和总抗氧化能力（T-AOC）（P<0.05）。2）注射diquat极显著降低仔猪的ADG和ADFI（P<0.01），极显著升高F/G（P<0.01）；饲粮添加COS显著抑制因注射diquat导致的ADG的下降（P<0.05）。3）注射diquat极显著降低仔猪的血浆过氧化氢酶（CAT）活性（P<0.01），显著降低空肠黏膜乳糖酶、蔗糖酶、麦芽糖酶的活性和葡萄糖转运载体2（GLUT2）、钠/葡萄糖转运载体1（SGLT1）的mRNA表达量（P<0.05）；饲粮添加COS显著升高氧化应激仔猪的血浆SOD活性和T-AOC（P<0.05），显著缓解空肠黏膜二糖酶活性的降低及GLUT2和SGLT1 mRNA表达量的下调（P<0.05）。由此可见，正常饲养条件下，饲粮添加50 mg/kg COS可显著改善仔猪对饲粮的养分消化率和机体的抗氧化能力，有降低F/G的趋势；氧化应激条件下，COS可通过改善机体的抗氧化能力，缓解diquat诱导的氧化应激，提高应激仔猪的空肠养分消化和转运能力，缓解氧化应激导致的增重下降。

关键词： 壳寡糖; 断奶仔猪; 抗氧化; 空肠黏膜; 养分消化和转运

本文引用格式

田刚, 黄琳惠, 宋晓华, 陈代文, 毛湘冰, 虞洁, 郑萍, 何军, 余冰 . 壳寡糖对氧化应激仔猪生长性能、抗氧化能力及空肠养分消化和转运能力的影响[J]. 动物营养学报, 2018 , 30(7) : 2652 -2661 . DOI: 10.3969/j.issn.1006-267x.2018.07.025

Abstract

This study was conducted to evaluate the effects of dietary chitooligosaccharides (COS) on growth performance, antioxidant capacity and nutrient digestion and transport capacity of jejunum of piglets under normal feeding and oxidative stress statues. Twenty four 24-day-old healthy Duroc×Landrace×Yorkshire weaned piglets with average body weight (BW) of (7.34±0.09) kg were randomly divided into control, COS, diquat, COS+diquat groups with 6 replicates per group and 1 pig per replicate by a 2×2 two factorial arrangement. The trial lasted for 28 days. COS was given all in the trial (50 mg/kg diet). Diquat challenged pigs were given a single intraperitoneal injection of 10 mg/kg BW diquat at the 22nd day and non-challenged pigs were injected intraperitoneally with the equal amount of physiological saline at the same time. Digestion trial was carried out from the 18th to 21st day by the method of endogenous indicator, blood were fasting sampled via anterior vena cava and then pigs were injected with diquat in the morning of the 22nd day, and pigs were slaughtered after blood sampled via anterior vena cava and jejunum mucosa samples were sampled in the morning of the 29th day. The results showed as follows:1) before diquat injection, there were no significant effects of dietary COS on average daily feed intake (ADFI) and average daily gain (ADG) of piglets (P>0.05), but the ratio of feed to gain (F/G) was tended to decreased (P=0.09). Dietary COS significantly increased the apparent digestibilities of dry matter, organism matter, crude protein, ether extract, total energy, ash, calcium and phosphor in diet of piglets (P<0.05), and significantly increased the activity of superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in plasma of piglets (P<0.05). 2) After diquat injection, ADG and ADFI of piglets were significantly decreased and F/G was significantly increased (P<0.01). Dietary COS significantly inhibited the reduction of ADG of piglets induced by diquat injection (P<0.05). 3) After diquat injection, the activity of catalase (CAT) in plasma (P<0.01) and lactase, sucrase and maltase of jejunum mucosa (P<0.05), and mRNA expression levels of glucose transporter 2 (GLUT2) and sodium/glucose transporter 1 (SGLT1) of jejunum mucosa (P<0.05) of piglets were significantly decreased. Dietary COS significantly increased the activity of SOD and T-AOC in plasma (P<0.05), and significantly relieved reduction of the activities of disaccharidases and mRNA expression levels of GLUT2 and SGLT1 of jejunum mucosa of oxidative stress piglets (P<0.05). In conclusion, under normal feeding statues, dietary 50 mg/kg COS can significantly improve nutrients digestibilities in diets and antioxidant capacity, decrease F/G of piglets. In oxidative stress statues, COS can improve antioxidant capacity, alleviate oxidative stress induced by diquat injection, improve nutrient digestion and transport capacity of jejunum and relieve the reduction of weight gain of oxidative stress piglets.

Key words： chitooligosaccharides; weaned piglets; antioxidant; jejunum mucosa; nutrient digestion and transport

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