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德氏乳酸菌对黄河鲤皮肤黏液中免疫和抗氧化指标以及皮肤中抗菌肽基因表达的影响

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  • 河南科技大学动物科技学院, 洛阳 471003
曾庆辉(1995-),男,河南信阳人,本科生,水族科学与技术专业。E-mail:13015579260@163.com

收稿日期: 2018-01-05

  网络出版日期: 2018-07-20

基金资助

河南科技大学大学生研究训练计划(SRTP)项目(2017313);河南科技大学博士科研启动金(13480074)

Effects of Lactobacillus delbrueckii on Immune and Antioxidant Indexes in Skin Mucous and Antibacterial Peptide Gene Expression in Skin of Cyprinus carpio Huanghe var.

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  • College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, China

Received date: 2018-01-05

  Online published: 2018-07-20

摘要

本试验主要研究了德氏乳酸菌(Lactobacillus delbrueckii)对黄河鲤(Cyprinus carpio Huanghe var.)皮肤黏液中免疫和抗氧化指标以及皮肤中抗菌肽基因表达的影响。将初重为(15.0±0.5)g的450尾黄河鲤随机分为5组,每组3个重复,每个重复30尾。5组试验鱼分别投喂含德氏乳酸菌0(对照组)、1×105、1×106、1×107和1×108 CFU/g的饲料,饲养8周后,采集皮肤黏液和皮肤,测定皮肤黏液中免疫和抗氧化指标以及皮肤中抗菌肽基因[肝表达抗菌肽1(Leap-1)和肝表达抗菌肽2(Leap-2)]的相对表达量。结果显示:1×106和1×107 CFU/g德氏乳酸菌组皮肤黏液中溶菌酶(LYZ)、碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)活性及补体3(C3)、补体4(C4)含量均显著高于对照组(P<0.05),同时其皮肤黏液中丙二醛(MDA)含量显著低于对照组(P<0.05),而1×105和1×108 CFU/g德氏乳酸菌组的上述指标与对照组差异不显著(P>0.05)。皮肤黏液中酸性磷酸酶(ACP)活性各组之间差异均不显著(P>0.05)。1×106 CFU/g德氏乳酸菌组皮肤黏液中过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPx)活性显著高于对照组(P<0.05),1×105、1×106和1×107 CFU/g德氏乳酸菌组皮肤黏液中总抗氧化能力(T-AOC)显著高于对照组(P<0.05)。各试验组皮肤中抗菌肽基因Leap-1和Leap-2的相对表达量均显著高于对照组(P<0.05),其中以1×107 CFU/g德氏乳酸菌组最高。结果表明,饲料中添加1×106~1×107 CFU/g的德氏乳酸菌能够提高黄河鲤皮肤的免疫功能和抗氧化能力,并能上调抗菌肽基因的表达。

本文引用格式

曾庆辉, 刘敏, 姚玉红, 丁翔飞, 史丽莎, 李晓凡, 张春暖 . 德氏乳酸菌对黄河鲤皮肤黏液中免疫和抗氧化指标以及皮肤中抗菌肽基因表达的影响[J]. 动物营养学报, 2018 , 30(7) : 2872 -2878 . DOI: 10.3969/j.issn.1006-267x.2018.07.050

Abstract

The aim of the present study was to investigate the effects of Lactobacillus delbrueckii (L. delbrueckii) on the immune and antioxidant indexes in skin mucous and the antimicrobial peptide gene expression in skin of Cyprinus carpio Huanghe var. A total of 450 Cyprinus carpio Huanghe var. with the initial body weight of (15.0±0.5) g were randomly distributed into five groups with three replicates per group and 30 fish per replicate. The fish in those five groups fed diets containing 0 (control group), 1×105, 1×106, 1×107 and 1×108 CFU/g L. delbrueckii, respectively, for 8 weeks. At the end of feeding trail, the skin mucous and skin were collected, and the immune and antioxidant indexes in the skin mucous and the relative expression levels of antimicrobial peptide genes including liver-expressed antimicrobial peptide-1 (Leap-1) and liver-expressed antimicrobial peptide-2 (Leap-2) in the skin were determined. The results showed that the activities of lysozyme (LYZ), alkaline phosphatase (AKP), superoxide dismutase (SOD) and the contents of complement 3 (C3) and complement 4 (C4) in skin mucous of the 1×106 and 1×107 CFU/g L. delbrueckii groups were significantly higher than those of the control group (P<0.05), while the malondialdehyde (MDA) content in skin mucous of the 1×106 and 1×107 CFU/g L. delbrueckii groups was significantly lower than that of the control group (P<0.05). There were no significant differences of above parameters between the 1×105 or 1×108 CFU/g L. delbrueckii groups and the control group (P>0.05). No significant difference was found in the acid phosphatase (ACP) activity in skin mucous among groups (P>0.05). The activities of catalase (CAT) and glutathion peroxidase (GPx) in skin mucous of the 1×106 CFU/g L. delbrueckii group were significantly higher than those of the control group (P<0.05), and the total antioxidant capacity (T-AOC) in skin mucous of the 1×105, 1×106 and 1×107 CFU/g L. delbrueckii groups was significantly higher than that of the control group (P<0.05). In addition, the relative expression levels of antimicrobial peptide genes Leap-1 and Leap-2 in skin of all experimental groups were significantly up-regulated compared with the control group (P<0.05), and highest values were all found in the 1×107 CFU/g L. delbrueckii group. Those results demonstrate that diet supplemented with 1×106 to 1×107 CFU/g L. delbrueckii can enhance the immunity, antioxidant capability and Leap-1 and Leap-2 gene expression in skin of Cyprinus carpio Huanghe var.

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