动物营养学报 >

2018 , Vol. 30 >Issue 11: 4369 - 4378

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.11.013

猪营养 Swine Nutrition

妊娠后期饲粮中添加小麦水解蛋白对母猪繁殖性能、血清生化指标和氨基酸含量及胎盘组织营养物质转运相关基因表达的影响

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  • 1. 中国农业大学动物科学技术学院, 北京 100193;
    2. 河南银发牧业有限公司, 郑州 451100;
    3. 郑州新威营养技术有限公司, 郑州 450100;
    4. 德克萨斯州农工大学动物科学院, 大学城 77843
寇娇(1993-),女,四川苍溪人,硕士研究生,从事动物营养与生化研究。E-mail:sckoujiao@163.com

收稿日期: 2018-05-03

  网络出版日期: 2018-11-20

基金资助

国家自然科学基金项目（31272450，31572412）；智慧郑州1125聚才计划

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Effects of Dietary Hydrolyzed Wheat Gluten in Late Pregnancy on Reproductive Performance, Serum Biochemical Parameters and Amino Acid Contents, and Placental Tissue Nutrient Transporters Related Gene Expression of Sows

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  • 1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
    2. Henan Yinfa Animal Husbandry Co., Ltd., Zhengzhou 451100, China;
    3. Zhengzhou Newwill Nutrition Technology Co., Ltd., Zhengzhou 450100, China;
    4. Department of Animal Science, Texas A & M University, College Station 77843, USA

Received date: 2018-05-03

  Online published: 2018-11-20

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摘要

本试验旨在研究妊娠后期饲粮中添加小麦水解蛋白（HWG）对母猪繁殖性能、血清生化指标和氨基酸含量及胎盘组织营养物质转运相关基因表达的影响。试验选用3~4胎次、预产期相近的妊娠母猪33头，随机分为3组，每组11个重复，每个重复1头母猪。对照组母猪饲喂基础饲粮，试验组母猪在基础饲粮中于妊娠第90~114天期间分别添加1%和2%的HWG，所有饲粮均等能等氮。结果表明：1）与对照组相比，饲粮中添加1% HWG显著提高了仔猪平均初生活仔重（P<0.05），饲粮中添加2% HWG显著提高了仔猪初生窝重、初生活仔窝重（P<0.05）。2）与对照组相比，饲粮中添加1%和2% HWG显著提高了妊娠第107天母猪血清中天冬酰胺、组氨酸、鸟氨酸含量和脐带血清中组氨酸、异亮氨酸、亮氨酸、苯丙氨酸和酪氨酸含量（P<0.05），显著降低了母猪血清中血氨、甘氨酸含量和脐带血清中血氨含量（P<0.05）；饲粮中添加1% HWG显著提高了母猪血清中缬氨酸含量（P<0.05），饲粮中添加2% HWG显著提高了母猪血清中异亮氨酸、苏氨酸含量和脐带血清中精氨酸、谷氨酸、谷氨酰胺、甘氨酸、蛋氨酸、丝氨酸、苏氨酸、色氨酸、缬氨酸、白蛋白、葡萄糖含量及碱性磷酸酶活性（P<0.05）。3）与对照组相比，饲粮中添加1% HWG显著提高了胎盘组织中性氨基酸转运载体2（SNAT2）、谷氨酸转运载体3（EAAT3）、L型氨基酸转运载体2（LAT2）、葡萄糖转运载体2（GLUT2）的mRNA相对表达量（P<0.05），饲粮中添加2% HWG显著提高了胎盘组织中性氨基酸转运载体1（SNAT1）、SNAT2、EAAT3、LAT2、小肽转运载体1（PepT1）、小肽转运载体2（PepT2）、葡萄糖转运载体1（GLUT1）、GLUT2、葡萄糖转运载体3（GLUT3）的mRNA相对表达量（P<0.05）。由此可见，妊娠后期饲粮中添加HWG能提高胎盘组织营养物质转运相关基因的表达，促进母体营养物质的转运效率和胎猪的生长发育，提高仔猪初生窝重。

关键词： 小麦水解蛋白; 妊娠母猪; 繁殖性能; 血清生化指标; 氨基酸; 胎盘

本文引用格式

寇娇, 梁海威, 刘宁, 戴兆来, 李菊, 潘俊良, 朱芳周, 伍国耀, 武振龙 . 妊娠后期饲粮中添加小麦水解蛋白对母猪繁殖性能、血清生化指标和氨基酸含量及胎盘组织营养物质转运相关基因表达的影响[J]. 动物营养学报, 2018 , 30(11) : 4369 -4378 . DOI: 10.3969/j.issn.1006-267x.2018.11.013

Abstract

This experiment was conducted to investigate the effects of dietary hydrolyzed wheat gluten (HWG) in late pregnancy on reproductive performance, serum biochemical parameters and amino acid contents, and placental tissue nutrient transporters related gene expression of sows. Thirty-three gestation sows (3 to 4 parities) with similar expected date of childbirth were randomly allocated to 3 groups with 11 replicates per group and 1 sow per replicate. Sows in the control group were fed a basal diet, and the others in the experimental groups were fed the basal diets supplemented with 1% and 2% HWG during day 90 to 114 of pregnancy, respectively. All diets were isonitrogenous and isocaloric. The results showed as follows:1) compared with the control group, dietary 1% HWG supplementation significantly increased the average birth weight of all piglets born alive of piglets (P<0.05), dietary 2% HWG supplementation significantly increased the total litter weight at birth for all piglets born and total litter weight at birth for all live piglets (P<0.05). 2) Compared with the control group, dietary 1% and 2% HWG supplementation significantly increased the contents of asparagine, histidine and ornithine in serum of sows on day 107 of pregnancy, and contents of histidine, isoleucine, leucine, phenylalanine and tyrosine in serum of umbilical cord on day 107 of pregnancy (P<0.05), whereas, significantly decreased the contents of ammonia, glycine in serum of sows and umbilical cord serum ammonia content (P<0.05); dietary 1% HWG supplementation significantly increased the serum valine content of sows (P<0.05), dietary 2% HWG supplementation significantly increased the contents of isoleucine and threonine in serum of sows, and contents of arginine, glusate, glutamine, glycine, methionine, serine, threonine, tryptophan, valine, albumin, glucose and alkaline phosphatase activity in serum of umbilical cord (P<0.05). 3) Compared with the control group, dietary 1% HWG supplementation significantly increased the mRNA relative expressions of sodium-dependent neutral amino acid transporter (SNAT)2, excitatoryamino acid transporter 3 (EAAT3), L-type amino acid transporter 2 (LAT2) and glucose transporter (GLUT)2 in placental tissue (P<0.05), dietary 2% HWG supplementation significantly increased the mRNA relative expressions of SNAT1, SNAT2, EAAT3, LAT2, peptide transporter (PepT)1, PepT2, GLUT1, GLUT2 and GLUT3 in placental tissue (P<0.05). In conclusion, dietary HWG in late pregnancy can improve the placental tissue nutrient transporters related gene expression, promote the efficiency of transport efficiency of maternal nutrients and the growth and development of fetal pigs, improve the total litter weight at birth for all piglets born.

Key words： hydrolyzed wheat gluten; gestation sows; reproductive performance; serum biochemical parameters; amino acids; placenta

参考文献

[1] WIESER H.Chemistry of gluten proteins[J].Food Microbiology,2007,24(2):115-119.  

[2] 王延州.小麦蛋白谷氨酰胺肽制备及对肠粘膜损伤修复效果的研究[D].硕士学位论文.北京:中国农业科学院,2014:13-16.

[3] DE SANTIS M A,GIULIANI M M,GIUZIO L,et al.Differences in gluten protein composition between old and modern durum wheat genotypes in relation to 20th century breeding in Italy[J].European Journal of Agronomy,2017,87:19-29.

[4] 何林玲.富含谷氨酰胺小麦水解蛋白的制备及其性质的研究[D].博士学位论文.武汉:武汉轻工大学,2015:40-49.

[5] WANG X Q,FENG Y,SHU G,et al.Effect of dietary supplementation with hydrolyzed wheat gluten on growth performance,cell immunity and serum biochemical indices of weaned piglets (Sus scrofa)[J].Agricultural Sciences in China,2011,10(6):938-945.  

[6] WU G,BAZER F W,JOHNSON G A,et al.Triennial growth symposium:important roles for L-glutamine in swine nutrition and production[J].Journal of Animal Science,2011,89(7):2017-2030.  

[7] WANG H,ZHANG C,WU G Y,et al.Glutamine enhances tight junction protein expression and modulates corticotropin-releasing factor signaling in the jejunum of weanling piglets[J].The Journal of Nutrition,2015,145(1):25-31.  

[8] WANG B,WU Z L,JI Y,et al.L-glutamine enhances tight junction integrity by activating CaMK kinase 2-AMP-activated protein kinase signaling in intestinal porcine epithelial cells[J].The Journal of Nutrition,2016,146(3):501-508.  

[9] JIAO N,WU Z L,JI Y,et al.L-glutamate enhances barrier and antioxidative functions in intestinal porcine epithelial cells[J].The Journal of Nutrition,2015,145(10):2258-2264.  

[10] SUN Y L,WU Z L,LI W,et al.Dietary L-leucine supplementation enhances intestinal development in suckling piglets[J].Amino Acids,2015,47(8):1517-1525.  

[11] 王立,戈蕾.小麦水解蛋白的生产及应用现状[J].饲料与畜牧,2016(10):47-49.

[12] 原伟伟,王井亮,代兵,等.小麦水解蛋白对早期断奶仔猪生产性能及肠道健康的影响[J].畜牧与兽医,2017,49(7):23-27.

[13] BLASCO M,FONDEVILA M,GUADA J A.Inclusion of wheat gluten as a protein source in diets for weaned pigs[J].Animal Research,2005,54(4):297-306.  

[14] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J].Method,2001,25(4):402-408.  

[15] BRETT K E,FERRARO Z M,YOCKELL-LELIEVRE J,et al.Maternal-fetal nutrient transport in pregnancy pathologies:the role of the placenta[J].International Journal of Molecular Sciences,2014,15(9):16153-16185.  

[16] LAGER S,POWELL T L.Regulation of nutrient transport across the placenta[J].Journal of Pregnancy,2012,2012:179827.

[17] WU G Y,BAZER F W,CUDD T A,et al.Maternal nutrition and fetal development[J].The Journal of Nutrition,2004,134(9):2169-2172.  

[18] MCPHERSON R L,JI F,WU G,et al.Growth and compositional changes of fetal tissues in pigs[J].Journal of Animal Science,2004,82(9):2534-2540.  

[19] 朱玉华.谷氨酰胺通过抑制microRNA-29a和细胞自噬调控宫内生长受限仔猪肠道发育的研究[D].博士学位论文.北京:中国农业大学,2017:84-104.

[20] PANTHAM P,ROSARIO F J,NIJLAND M,et al.Reduced placental amino acid transport in response to maternal nutrient restriction in the baboon[J].American Journal of Physiology:Regulatory,Integrative and Comparative Physiology,2015,309(7):R740-R746.

[21] ILLSLEY N P.Current topic:glucose transporters in the human placenta[J].Placenta,2000,21(1):14-22.  

[22] WU G Y.Functional amino acids in growth,reproduction,and health[J].Advances in Nutrition,2010,1(1):31-37.  
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