动物营养学报 >

2018 , Vol. 30 >Issue 11: 4470 - 4481

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.11.024

反刍动物营养 Ruminant Nutrition

干酒糟及其可溶物中糠氨酸含量与瘤胃降解特性和瘤胃非降解蛋白质的小肠消化率的相关性研究

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  • 东北农业大学动物科学技术学院, 哈尔滨 150030
徐宏建(1994-),男,黑龙江哈尔滨人,硕士研究生,从事反刍动物营养与饲料科学研究。E-mail:xuhongjian0714@163.com

收稿日期: 2018-04-25

  网络出版日期: 2018-11-20

基金资助

国家奶牛产业技术体系（CARS-36）

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Correlation Research between Furosine Content and Ruminal Degradation Characteristics and Intestinal Digestibility of Rumen Undegraded Protein in Distillers Dried Grains with Soluble

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  • College of Animal Science and Technology of Northeast Agricultural University, Harbin 150030, China

Received date: 2018-04-25

  Online published: 2018-11-20

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摘要

本试验旨在验证美拉德反应中间产物糠氨酸含量与不同程度热加工干酒糟及其可溶物（DDGS）的瘤胃降解特性及瘤胃非降解蛋白质的小肠消化率之间存在相关性并建立回归方程，并与酸性洗涤不溶蛋白质作为饲料热处理敏感指标进行比较。试验采用高效液相色谱法测定不同热处理DDGS中糠氨酸含量，采用尼龙袋法测定干物质和粗蛋白质瘤胃降解特性并采用改进的三步体外法测定瘤胃非降解蛋白质的小肠消化率，分析两者之间相关性并建立回归方程。结果表明：1）随着加热程度的增加，DDGS中糠氨酸和酸性洗涤不溶蛋白质含量显著增加（P<0.05），干物质和粗蛋白质瘤胃有效降解率及瘤胃非降解蛋白质的小肠消化率降低，且不同处理之间存在显著差异（P<0.05）。2）糠氨酸与酸性洗涤不溶蛋白质含量均与干物质和粗蛋白质瘤胃培养可溶部分（r=-0.72 vs.-0.60；r=-0.60 vs.-0.51）、不可降解部分（r=0.96 vs.0.84；r=0.96 vs.0.85）、有效降解率（r=-0.62 vs.-0.51；r=-0.72 vs.-0.61）以及瘤胃非降解蛋白质的小肠消化率（r=-0.52 vs.-0.57）存在显著相关性（P<0.05），并可建立回归预测方程。3）糠氨酸含量较酸性洗涤不溶蛋白质含量与瘤胃降解特性具有更高的相关性，二者与瘤胃非降解蛋白质的小肠消化率、总可消化蛋白质含量之间的相关性相似，但糠氨酸含量与小肠可消化蛋白质含量具有更高相关性（r=0.67）。综上，不同热处理DDGS中糠氨酸含量与瘤胃降解特性和小肠消化率存在相关关系，并可建立回归方程。糠氨酸含量较酸洗洗涤不溶蛋白质含量更适合作为评价饲料热加工程度的关键指标。

关键词： 糠氨酸; 瘤胃降解特性; 小肠消化率; DDGS; 热加工

本文引用格式

徐宏建, 李昕, 王玉洁, 韩春雷, 张永根 . 干酒糟及其可溶物中糠氨酸含量与瘤胃降解特性和瘤胃非降解蛋白质的小肠消化率的相关性研究[J]. 动物营养学报, 2018 , 30(11) : 4470 -4481 . DOI: 10.3969/j.issn.1006-267x.2018.11.024

Abstract

The aim of this experiment was to verify the correlation between the furosine content of Maillard reaction intermediates and the rumen degradation characteristic, intestinal digestibility of rumen undegraded protein of distillers dried grains with soluble (DDGS) with different degrees of heat processing, to establish regression equations, and to compare with the acid detergent insoluble protein as a sensitive index for heat treatment of feeds. In this study, high-performance liquid chromatography was used to determine furosine content, nylon bag method was used to determine the rumen degradation characteristics of dry matter and crude protein, and a modified three-step in vitro method was used to determine the intestinal digestibility of rumen undegraded protein in DDGS with different degrees of heat processing, to analyze correlation and establish regression equations. The results showed as follows:1) with increasing heating degree, the furosine and acid detergent insoluble protein contents in DDGS significantly increased (P<0.05), and the rumen degradation rate and intestinal digestibility of undegraded protein of dry matter and crude protein in DDGS significantly decreased (P<0.05). 2) Both furosine and acid detergent insoluble protein contents were significantly related to soluble fraction of dry matter and crude protein (r=-0.72 vs. -0.60; r=-0.60 vs. -0.51), undegradable fraction (r=0.96 vs. 0.84; r=0.96 vs. 0.85), effective degradation rate (r=-0.62 vs. -0.51; r=-0.72 vs. -0.61) and intestinal digestibility of rumen undegradable protein (r=-0.52 vs. -0.57), and the correlation was significant (P<0.05). The prediction equations could be established by the regression. 3) Furosine content had a higher correlation with ruminal degradation characteristics than acid detergent insoluble protein content. Both had similar correlations with the intestinal digestibility of rumen undegradable protein and total digestible protein content, but furosine and small intestine digestible protein content had a higher correlation(r=0.67). There is a correlation between furosine content and ruminal degradation characteristics and intestinal digestibility in DDGS with different heat processing, and a regression equation can be established. Furosine content is more suitable than acid detergent insoluble protein content as a key indicator to evaluate the degree of heat processing.

Key words： furosine; rumen degradable characteristics; intestinal digestibility; DDGS; heat processing

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