动物营养学报 >

2018 , Vol. 30 >Issue 11: 4559 - 4568

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.11.032

特种经济动物营养 Special economic animal nutrition

半胱胺盐酸盐对冬毛期雄性水貂毛皮品质、血清生化和激素指标及肝脏相关基因表达的影响

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  • 1. 中国农业科学院特产研究所, 吉林省特种经济动物分子生物学国家重点实验室, 长春 130112;
    2. 牧原食品科技股份有限公司, 洛阳 420106;
    3. 山东亚康检测技术有限公司, 潍坊 261101
孙伟丽(1982-),女,黑龙江牡丹江人,博士研究生,研究方向为特种经济动物营养与饲养。E-mail:tcsswl@163.com

收稿日期: 2018-04-19

  网络出版日期: 2018-11-20

基金资助

吉林省科技发展计划项目（20150101112JC）；中国农业科学院科技创新工程经费资助

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Effects of Cysteamine Hydrochloride on Fur Quality, Serum Biochemical and Hormone Parameters and Liver Related Gene Expression of Male Minks during Winter Fur-Growing Period

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  • 1. Institute of Special Animal and Plant Sciences, Chinese Academy of Agriculture Sciences, Jilin Provincial Key Laboratory for Molecular Biology of Special Economic Animals, Changchun 130112, China;
    2. Muyuan Food Technology Co., Ltd., Luoyang 420106, China;
    3. Shandong Yakang Detection Technology Co., Ltd., Weifang 261101, China

Received date: 2018-04-19

  Online published: 2018-11-20

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摘要

本试验旨在研究饲粮中添加半胱胺盐酸盐（CSH）对冬毛期雄性水貂毛皮品质、血清生化和激素指标及肝脏相关基因表达的影响。选择160日龄体重[（2.13±0.10）kg]相近的健康冬毛期雄性水貂56只，随机分为7组，每组8个重复，每个重复1只。各组分别饲喂在基础饲粮中添加0（Ⅰ组，对照组）、60（Ⅱ组）、90（Ⅲ组）、120（Ⅳ组）、60（Ⅴ组）、90（Ⅵ组）、120 mg/kg（Ⅶ组）CSH的试验饲粮；其中，Ⅱ、Ⅲ、Ⅳ组添加方式为连续添加，Ⅴ、Ⅵ、Ⅶ组添加方式为间隔添加（连续添加1周，间隔1周）。预试期7 d，正试期51 d。结果表明：1）Ⅵ和Ⅶ组水貂皮长、针毛长度和绒毛长度显著或极显著高于对照组（P<0.05或P<0.01）。2）Ⅱ、Ⅲ、Ⅴ、Ⅵ、Ⅶ组血清尿素氮含量显著或极显著低于对照组（P<0.05或P<0.01），Ⅱ、Ⅲ、Ⅴ、Ⅵ、Ⅶ组血清甘油三酯含量显著或极显著低于对照组（P<0.05或P<0.01）。3）Ⅵ组血清生长激素含量显著高于对照组和Ⅳ组（P<0.05），Ⅱ、Ⅲ、Ⅵ组血清生长抑素含量显著低于对照组和Ⅳ组（P<0.05），Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ、Ⅶ组血清生长激素受体含量显著或极显著高于对照组（P<0.05或P<0.01），Ⅱ、Ⅲ、Ⅴ、Ⅵ、Ⅶ组血清胰岛素样生长因子-Ⅰ含量显著高于Ⅳ组（P<0.05）。4）Ⅵ组肝脏胰岛素样生长因子-Ⅰ基因表达量显著高于对照组和Ⅱ、Ⅳ、Ⅴ组（P<0.05），Ⅵ组肝脏胰岛素样生长因子-Ⅰ受体基因表达量显著高于Ⅱ、Ⅳ、Ⅴ组（P<0.05），Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ、Ⅶ组肝脏生长激素受体基因表达量显著或极显著高于对照组（P<0.05或P<0.01）。由此可见，在本试验条件下，冬毛期雄性水貂饲粮中CSH的适宜添加水平为90 mg/kg，适宜添加方式为间隔添加。

关键词： 半胱胺盐酸盐; 水貂; 毛皮品质; 血清指标; 基因表达

本文引用格式

孙伟丽, 樊燕燕, 张婷, 王卓, 李恒伟, 李光玉 . 半胱胺盐酸盐对冬毛期雄性水貂毛皮品质、血清生化和激素指标及肝脏相关基因表达的影响[J]. 动物营养学报, 2018 , 30(11) : 4559 -4568 . DOI: 10.3969/j.issn.1006-267x.2018.11.032

Abstract

This experiment was conducted to investigate the effects of dietary cysteamine hydrochloride (CSH) on fur quality, serum biochemical and hormone parameters and liver related gene expression of male minks during winter fur-growing period. Fifty six 160-day-old male minks with similar body weight[(2.13±0.10) kg] were randomly divided into 7 groups with 8 replicates per group and 1 mink per replicate. Minks were fed the basal diets supplemented with 0 (group Ⅰ, control group) 60 (group Ⅱ), 90 (group Ⅲ), 120 (group Ⅳ), 60 (group Ⅴ), 90 (group Ⅵ) and 120 mg/kg (group Ⅶ) CSH, respectively; the supplemental way of groups Ⅱ, Ⅲ and Ⅳ was continuous supplementation, and the supplemental way of groups Ⅴ, Ⅵ and Ⅶ was interval supplementation (1 week continuous, 1 weeks interval). The pre-experimental period lasted for 7 days, and the experimental period lasted for 51 days. The results showed as follows:1) the skin length, aciculum length and villus length of minks in groups Ⅵ and Ⅶ were significantly higher than those in the control group (P<0.05 or P<0.01). 2) The serum urea nitrogen content in groups Ⅱ, Ⅲ, Ⅴ, Ⅵ and Ⅶ was significantly lower than that in the control group (P<0.05 or P<0.01), and the serum triglyceride content in groups Ⅱ, Ⅲ, Ⅴ, Ⅵ and Ⅶ was significantly lower than that in the control group (P<0.05 or P<0.01). 3) The serum growth hormone content in group Ⅵ was significantly higher than that in the control group and group Ⅳ (P<0.05), and the serum somatostatin content in groups Ⅱ, Ⅲ and Ⅵ was significantly lower than that in the control group and group Ⅳ (P<0.05), the serum growth hormone receptor content in groups Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅵ and Ⅶ was significantly higher than that in the control group (P<0.05 or P<0.01), the serum insulin-like growth factor-Ⅰ content in groups Ⅱ, Ⅲ, Ⅴ, Ⅵ and Ⅶ was significantly higher than that in the control Ⅳ (P<0.05 or P<0.01). 4) The liver insulin-like growth factor-Ⅰ gene expression level in group Ⅵ was significantly higher than that in the control group and groups Ⅱ, Ⅳ, Ⅴ (P<0.05), the liver insulin-like growth factor-Ⅰ receptor gene expression level in group Ⅵ was significantly higher than that in groups Ⅱ, Ⅳ, Ⅴ (P<0.05), the liver growth hormone receptor gene expression level in groups Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅵ and Ⅶ was significantly higher than that in the control group (P<0.05 or P<0.01). In conclusion, in this experimental condition, the optimal CSH supplemental level for diets of male minks during winter fur-growing period is 90 mg/kg, and the optimal supplemental way was interval supplementation.

Key words： cysteamine hydrochloride; minks; fur quality; serum parameters; gene expression

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