动物营养学报 >

2018 , Vol. 30 >Issue 11: 4601 - 4610

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.11.036

饲料营养 Feed science and technology

饲粮中添加高剂量茶多酚对产蛋后期蛋鸡生产性能、蛋品质和脂质代谢的影响

展开
  • 四川农业大学动物营养研究所, 动物抗病营养教育部重点实验室, 动物抗病营养与饲料农业部重点实验室, 成都 611130
何俊金(1991-),男,四川绵阳人,硕士研究生,从事家禽营养研究。E-mail:413899685@qq.com

收稿日期: 2018-04-17

  网络出版日期: 2018-11-20

基金资助

国家科技支撑计划（2014BAD13B04）；四川省蛋鸡产业链项目（2014NZ0002）；四川省科创饲料产业技术研究院（2013NZ0054）

收起

Effects of Adding High Doses of Tea Polyphenols into Diets on Performance, Egg Quality and Lipid Metabolism of Laying Hens during Later Laying Period

Expand
  • Key Laboratory for Animal Disease-Resistance Nutrition and Feed of Ministry, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2018-04-17

  Online published: 2018-11-20

Fold

摘要

本试验旨在研究饲粮中添加高剂量茶多酚对产蛋后期蛋鸡生产性能、蛋品质、血清脂质代谢指标和肝脏脂质代谢相关基因表达的影响。选用480羽65周龄罗曼粉壳蛋鸡作为试验动物，随机分成4组，每组8个重复，每个重复15羽。对照组饲喂基础饲粮，3个试验组分别饲喂在基础饲粮中添加666、1 333和2 666 mg/kg茶多酚（纯度为63.58%，其中儿茶素含量为40.20%）的试验饲粮，预试期2周，正试期9周。结果显示：1）与对照组相比，添加2 666 mg/kg茶多酚显著降低产蛋率（第1~3周、第4~6周和第1~9周）、平均日采食量（第1~3周、第4~6周、第7~9周和第1~9周），显著升高料蛋比（第1~3周和第4~6周）；1 333和2 666 mg/kg茶多酚组的平均蛋重在第1~3周、第4~6周和第1~9周显著低于对照组（P<0.05）。2）与对照组相比，饲粮添加2 666 mg/kg茶多酚显著提高第2周哈夫单位（P<0.05），且显著降低第2周蛋壳比率（P<0.05）；饲粮添加1 333 mg/kg茶多酚显著提高第4周哈夫单位（P<0.05），且显著降低第2周蛋壳比率（P<0.05）；饲粮添加666、1 333和2 666 mg/kg茶多酚均显著降低第9周蛋黄胆固醇含量（P<0.05）；饲粮添加666、1 333和2 666 mg/kg茶多酚对蛋壳强度、蛋黄颜色、蛋黄比率、蛋白比率及蛋黄脂肪酸组成均无显著影响（P>0.05）。3）与对照组相比，饲粮添加666、1 333和2 666 mg/kg茶多酚显著降低血清总胆固醇、低密度脂蛋白胆固醇含量（P<0.05），饲粮添加1 333和2 666 mg/kg茶多酚显著降低血清甘油三酯含量（P<0.05）。4）与对照组相比，饲粮添加666、1 333和2 666 mg/kg茶多酚显著提高肝脏腺苷酸激活蛋白激酶（AMPK）mRNA相对表达量（P<0.05）。由此可知，饲粮中添加高剂量（1 333和2 666 mg/kg）的茶多酚会降低蛋鸡产蛋后期的生产性能，但可以提高鸡蛋的哈夫单位，降低血清总胆固醇和低密度脂蛋白胆固醇含量及蛋黄胆固醇含量，并上调肝脏AMPK基因的表达。

关键词： 茶多酚; 蛋鸡; 生产性能; 蛋品质; 脂质代谢

本文引用格式

何俊金, 王建萍, 丁雪梅, 曾秋凤, 白世平, 张克英 . 饲粮中添加高剂量茶多酚对产蛋后期蛋鸡生产性能、蛋品质和脂质代谢的影响[J]. 动物营养学报, 2018 , 30(11) : 4601 -4610 . DOI: 10.3969/j.issn.1006-267x.2018.11.036

Abstract

This experiment was conducted to study the effects of adding high doses of tea polyphenols (TP) into diets on performance, egg quality and serum lipometabolism parameters and hepatic lipometabolism-related gene expression of laying hens during later laying period. A total of 480 Lohmann laying hens (65-week-old) were randomly allotted into 4 groups with 8 replicates per group and 15 hens per replicate. The laying hens in control group were fed a basal diet, and those in experimental groups were fed the basal diets supplemented with 666, 1 333 and 2 666 mg/kg TP (its purity reached 63.58%, and the content of catechin in it was 40.20%), respectively. There was a pretrial period of 2 weeks followed by an experimental period of 9 weeks. The results showed as follows:1) compared with the control group, adding 2 666 mg/kg TP significantly decreased the laying rate of week 1 to 3, 4 to 6 and 1 to 9 (P<0.05), significantly decreased the average daily feed intake of week 1 to 3, 4 to 6, 7 to 9 and 1 to 9 (P<0.05), and significantly increased the feed/egg of week 1 to 3 and 4 to 6 (P<0.05); adding 1 333 and 2 666 mg/kg TP significantly decreased the average egg weight of week 1 to 3, 4 to 6 and 1 to 9 (P<0.05). 2) Compared with the control group, adding 2 666 mg/kg TP significantly increased the Haugh unit of week 2 (P<0.05), and significantly decreased the eggshell ratio of week 2 (P<0.05); adding 1 333 mg/kg TP significantly increased the Haugh unit of week 4 (P<0.05), and significantly decreased the eggshell ratio of week 2 (P<0.05); adding 666, 1 333 and 2 666 mg/kg TP significantly decreased egg yolk cholesterol content of week 9 (P<0.05); adding 666, 1 333 and 2 666 mg/kg TP did not significantly affect the eggshell strength, egg yolk color, egg yolk ratio, albumen ratio and egg yolk fatty acid composition (P>0.05). 3) Compared with the control group, adding 666, 1 333 and 2 666 mg/kg TP significantly decreased the serum total cholesterol (TC) and low density lipoprotein cholesterin (LDL-C) contents (P<0.05); adding 1 333 and 2 666 mg/kg TP significantly decreased the serum triglyceride (TG) content (P<0.05). 4) Compared with the control group, adding 666, 1 333 and 2 666 mg/kg TP significantly increased the mRNA relative expression level of AMP-activated protein kinase (AMPK) in liver (P<0.05). In conclusion, adding high doses (1 333 and 2 666 mg/kg) of TP into diets cause the decrease of performance of laying hens during later laying period, but can increase the Haugh unit of egg, decrease the serum TC and LDL-C contents and egg yolk cholesterol content, and up-regulate the expression of AMPK gene in liver.

Key words： tea polyphenols; laying hens; performance; egg quality; lipid metabolism

参考文献

[1] DEVINE P J,PERREAULT S D,LUDERER U.Roles of reactive oxygen species and antioxidants in ovarian toxicity[J].Biology of Reproduction,2012,86(2):1-10.

[2] YANG C S,LAMBERT J D,SANG S M.Antioxidative and anti-carcinogenic activities of tea polyphenols[J].Archives of Toxicology,2009,83(1):11-21.  

[3] 张光玉.茶多酚——一种极有潜力的新型饲料添加剂[J].饲料博览,1999(11):7-10.

[4] UUGANBAYAR D,BAE I H,CHOI K S,et al.Effects of green tea powder on laying performance and egg quality in laying hens[J].Asian-Australasian Journal of Animal Sciences,2005,18(12):1769-1774.  

[5] 楼洪兴,林智,王友明,等.茶多酚对蛋鸡生产性能、脂类代谢及蛋品品质的影响[J].茶叶科学,2004,24(2):135-140.

[6] 张旭,蒋桂韬,王向荣,等.茶多酚对蛋鸡生产性能、蛋品质和蛋黄胆固醇含量的影响[J].动物营养学报,2011,23(5):869-874.

[7] BISWAS M A H,WAKITA M.Comparison of two dietary factors,green tea powder feeding and feed restriction,influencing laying performance and egg quality in hens[J].Faculty of Bioresources Mie University,2001,25(26):55-61.

[8] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method[J].Methods,2001,25(4):402-408.  

[9] YAMANE T,GOTO H,TAKAHASHI D,et al.Effects of hot water extracts of tea on performance of laying hens[J].Japanese Poultry Science,1999,36(1):31-37.  

[10] SADAO K,YUKO Y.Effects of green tea powder feed supplement on performance of hens in the late stage of laying[J].International Journal of Poultry Science,2008,7(5):491-496.  

[11] 王人悦.绿茶粉及茶多酚对蛋鸡血液生化指标、生产性能及鸡蛋品质的影响[D].硕士学位论文.合肥:安徽农业大学,2007.

[12] 汪小红,武书庚,崔耀明,等.茶多酚对蛋鸡生产性能、蛋品质和抗氧化能力的影响[J].动物营养学报,2017,29(1):193-201.

[13] 尹靖东,齐广海,霍启光.类黄酮对蛋鸡脂类代谢的影响[J].畜牧兽医学报,2002,33(3):215-220.

[14] 吕娜.茶叶的生物活性成分及其生物学作用[J].产业与科技论坛,2014(21):63-64.

[15] 岳三立.饲料中主要抗营养因子作用机理及其防治措施[J].农家科技旬刊,2016(11):366.

[16] TSUANG Y H,SUN J S,CHEN L T,et al.Direct effects of caffeine on osteoblastic cells metabolism:the possible causal effect of caffeine on the formation of osteoporosis[J].Journal of Orthopaedic Surgery and Research,2006,1:7.

[17] HALLSTRÖM H,WOLK A,GLYNN A,et al.Coffee,tea and caffeine consumption in relation to osteoporotic fracture risk in a cohort of Swedish women[J].Osteoporosis International,2006,17(7):1055-1064.  

[18] 张兰威.蛋制品工艺学[M].哈尔滨:黑龙江科学技术出版社,1996.

[19] ROBERTS J R.Factors affecting egg shell and internal egg quality[C]//LE MERIDIEN S R.18th Annual ASAIM Southeast Asian Feed Technology and Nutrition Workshop.Cambodia:ASAIM,2010.

[20] PUTHPONGSIRIPORN U,SCHEIDELER S E,SELL J L,et al.Effects of vitamin E and C supplementation on performance,in vitro lymphocyte proliferation,and antioxidant status of laying hens during heat stress[J].Poultry Science,2001,80(8):1190-1200.  

[21] 高斌,高洪.氧自由基与细胞损伤[J].动物医学进展,2002,23(5):34-36.

[22] 曹鹤瑶,何伟逸,王琳琳,等.绿茶作为肠道内脂质消化吸收抑制剂的研究进展[J].食品科技,2013,38(11):62-65.

[23] 齐广海,郑君杰,尹靖东,等.类黄酮物质对蛋鸡抗氧化和脂质代谢的影响[J].营养学报,2002,24(2):153-157.

[24] 王继强,龙强,李爱琴,等.降低鸡蛋胆固醇的营养调控措施[J].家禽科学,2008(9):39-42.

[25] PEARCE J.Some differences between avian and mammaeian biochemistry[J].International Journal of Biochemistry,1977,8(4):269-275.  

[26] HARDIE D G,CARLING D,CARLSON M.The AMP-activated/SNF1 protein kinase subfamily:metabolic sensors of the eukaryotic cell?[J].Annual Review of Biochemistry,1998,67(1):821-855.  

[27] WINDER W W,HARDIE D G.Inactivation of acetyl-CoA carboxylase and activation of AMP-activated protein kinase in muscle during exercise[J].American Journal of Physiology,1996,270(2 Pt 1):E299-E304.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2