动物营养学报 >

2018 , Vol. 30 >Issue 11: 4660 - 4669

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.11.043

饲料营养 Feed science and technology

富硒酵母和枯草芽孢杆菌对湖羊羔羊小肠黏膜形态和直肠菌群的影响

展开
  • 安徽农业大学动物科技学院, 安徽地方畜禽遗传资源保护与生物育种省级实验室, 合肥 230036
程连平(1994-),男,山东济南人,硕士研究生,研究方向为动物临床诊疗技术。E-mail:chenglianping8@163.com

收稿日期: 2018-04-16

  网络出版日期: 2018-11-20

基金资助

2018年安徽省重点研究与开发计划（1804g07020187）；地方畜禽遗传资源保护与生物育种安徽省重点实验室开放课题（AKLGRCB2017009）；安徽省现代农业牛羊产业技术体系（AHCYJSTX-07）

收起

Effects of Selenium-Enriched Yeast and Bacillus subtilis on Intestinal Mucosal Morphology and Rectum Microflora of Hu Lambs

Expand
  • College of Animal Science and Technology, The Open Fund of Anhui Province Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, Anhui Agricultural University, Hefei 230036, China

Received date: 2018-04-16

  Online published: 2018-11-20

Fold

摘要

本试验旨在研究富硒酵母和枯草芽孢杆菌对湖羊断奶羔羊小肠黏膜形态和直肠菌群的影响。选取体况良好、体重为（9.65±0.38）kg的湖羊断奶羔羊21只，随机分为3组，即对照组、富硒酵母组和枯草芽孢杆菌组，每组7只羊。对照组饲喂基础饲粮，试验组分别按照100 g/t的比例在精料中添加富硒酵母和枯草芽孢杆菌制剂。试验期为28 d。结果表明：1）与对照组相比，富硒酵母组和枯草芽孢杆菌组的十二指肠、空肠、回肠的绒毛高度显著提高（P<0.05），十二指肠和回肠的隐窝深度显著降低（P<0.05），空肠的隐窝深度极显著降低（P<0.01），十二指肠和空肠的绒毛高和隐窝深度比值（V/C）极显著提高（P<0.01），回肠V/C显著提高（P<0.05）。2）富硒酵母和枯草芽孢杆菌的添加影响了湖羊羔羊直肠菌群的α多样性，使得直肠菌群在纲、目、科、属、种水平上呈现不同的丰度差异。综上得出，在饲粮中添加富硒酵母和枯草芽孢杆菌能够促进湖羊羔羊小肠各段的发育，增加直肠有益菌群的丰度，降低有害菌群的增殖。

关键词： 富硒酵母; 枯草芽孢杆菌; 湖羊羔羊; 小肠黏膜形态; 直肠菌群

本文引用格式

程连平, 舒迎霜, 贺濛初, 夏晓冬, 李玉, 冯士彬, 王希春, 吴金节 . 富硒酵母和枯草芽孢杆菌对湖羊羔羊小肠黏膜形态和直肠菌群的影响[J]. 动物营养学报, 2018 , 30(11) : 4660 -4669 . DOI: 10.3969/j.issn.1006-267x.2018.11.043

Abstract

The present experiment was conducted to evaluate the effects of selenium-enriched yeast and Bacillus subtilis on intestinal mucosal morphology and rectum microflora of Hu lambs. Twenty-one Hu Lambs, weighted (9.65±0.38) kg, were divided into 3 groups:control group, selenium-enriched yeast group and Bacillus subtilis group, with 7 lambs per group. The lambs in the control group were fed a basal diet, and in experimental groups, 100 g/t selenium-enriched yeast and Bacillus subtilis were added into concentrate, respectively. The experiment lasted for 28 days. The results showed as follows:1) compared with the control group, the villus height of duodenum, jejunum and ileum in the selenium enriched yeast group and Bacillus subtilis group significantly increased (P<0.05); the crypt depth of duodenum and ileum significantly decreased (P<0.05), while the crypt depth of jejunum significantly decreased (P<0.01); the villus height to crypt depth ratio (V/C) of duodenum and jejunum significantly increased (P<0.01), and the V/C of ileum significantly increased (P<0.05). 2) The supplement of selenium-enriched yeast and Bacillus subtilis affected the α diversity of the rectal flora of Hu lambs, resulting in different abundances of the rectal flora at the levels of the class, order, family, genus, species. It is concluded that the supplement of selenium enriched yeast and Bacillus subtilis in the diet can significantly enhance the development of the small intestine of lambs, increase the abundance of beneficial rectum microflora and reduce the proliferation of harmful bacteria.

Key words： selenium-enriched yeast; Bacillus subtilis; Hu lambs; intestinal mucosal morphology; rectum microflora

参考文献

[1] 杨晓洪,王娜,叶波平.畜禽养殖中的抗生素残留以及耐药菌和抗性基因研究进展[J].药物生物技术,2014(6):583-588.

[2] SÁNCHEZ B,DELGADO S,BLANCO-MÍGUEZ A,et al.Probiotics,gut microbiota,and their influence on host health and disease[J].Molecular Nutrition & Food Research,2016,61(1),doi:10.1002/mnfr.201600240.

[3] JUNIPER D T,PHIPPS R H,RAMOS-MORALES E,et al.Effect of dietary supplementation with selenium-enriched yeast or sodium selenite on selenium tissue distribution and meat quality in beef cattle[J].Journal of Animal Science,2008,86(11):3100-3109.  

[4] MOLNAR A K,PODMANICZKY B,KURTI K,et al.Effect of different concentrations of Bacillus subtilis on growth performance,carcase quality,gut microflora and immune response of broiler chickens[J].British Poultry Science,2011,52(6):658-665.  

[5] 张宏福.动物营养参数与饲养标准[M].2版.北京:中国农业出版社,2010.

[6] CHEN Z,XIE J,WANG B,et al.Effect of γ-aminobutyric acid on digestive enzymes,absorption function,and immune function of intestinal mucosa in heat-stressed chicken[J].Poultry Science,2014,93(10):2490-2500.  

[7] 贺琴,王自蕊,游金明,等.酵母壁多糖对断奶仔猪生长性能和小肠黏膜形态结构的影响[J].动物营养学报,2016,28(11):3536-3541.

[8] 秦红.益生菌对育肥猪肠道屏障功能的影响[D].硕士学位论文.太谷:山西农业大学,2016.

[9] YUNUSOVA R D,NEVILLE T L,VONNAHME K A,et al.Impacts of maternal selenium supply and nutritional plane on visceral tissues and intestinal biology in 180-day-old offspring in sheep[J].Journal of Animal Science,2013,91(5):2229-2242.  

[10] 齐博,武书庚,王晶,等.枯草芽孢杆菌对肉仔鸡生长性能、肠道形态和菌群数量的影响[J].动物营养学报,2016,28(6):1748-1756.

[11] MAHMOUD K Z,OBEIDAT B S,AL-SADI M Z,等.枯草芽孢杆菌及饲料粗蛋白质水平对肉鸡生长性能和肉鸡肠道形态的影响[J].饲料博览,2017(1):56.

[12] 祁凤华,杨帆,马红,等.枯草芽孢杆菌与嗜酸乳杆菌对黄羽肉鸡小肠黏膜形态和免疫器官指数的影响[J].黑龙江畜牧兽医,2015(19):129-131.

[13] 周勃.枯草芽孢杆菌对肉鸡小肠黏膜组织形态结构的影响[J].国外畜牧学(猪与禽),2017,37(8):75-77.

[14] ROUND J L,MAZMANIAN S K.The gut microbiota shapes intestinal immune responses during health and disease[J].Nature Reviews Immunology,2009,9(5):313-323.  

[15] LV C H,WANG T,REGMI N,et al.Effects of dietary supplementation of selenium-enriched probiotics on production performance and intestinal microbiota of weanling piglets raised under high ambient temperature[J].Journal of Animal Physiology and Animal Nutrition,2015,99(6):1161-1171.  

[16] LI S Y,ZHOU S,WANG X,et al.Yeast surface display of Escherichia coli enterotoxin and its effects of intestinal microflora and mucosal immunity[J].Current Microbiology,2017,74(7):854-862.  

[17] 刘红露,范磊,戴茜茜,等.面包酵母硒与酪酸梭菌制剂对肉鸡生长、抗氧化和肠道菌群的影响[J].浙江农业学报,2015,27(9):1529-1534.

[18] LI W F,RAJPUT I R,XU X,et al.Effects of probiotic(Bacillus subtilis)on laying performance,blood biochemical properties and intestinal microflora of Shaoxing duck[J].International Journal of Poultry Science,2011,10(8):583-589.  

[19] 郑婷婷,王苇,秦瑶,等.日粮添加耐受性枯草芽孢杆菌Q04对肉鸡肠道菌群、生产性能、部分血液生化指标和免疫指标的影响[C]//中国畜牧兽医学会动物微生态学分会第十一次全国学术研讨会暨第五届会员代表大会论文集.重庆.中国畜牧兽医学会动物微生态学分会,2014:147-147.

[20] WU B Q,ZHANG T,GUO L Q,et al.Effects of Bacillus subtilis KD1 on broiler intestinal flora[J].Poultry Science,2011,90(11):2493-2499.  

[21] 吴志新,冯雪,曲艺,等.枯草芽孢杆菌对草鱼生长、消化和肠道菌群的影响[C]//第九届世界华人鱼虾营养学术研讨会论文集.厦门:中国水产学会,2013:350-350.

[22] 任继平.芽孢杆菌制剂对大肠杆菌感染仔猪免疫应答及肠道菌群影响[D]博士学位论文.北京:中国农业大学,2014.

[23] SANZ Y,NADAL I,SANCHEZ E.Probiotics as drugs against human gastrointestinal infections[J].Recent Patents on Anti-Infective Drug Discovery,2007,2(2):148-156.  

[24] LJUNGH A,WADSTRÖM T.Lactic acid bacteria as probiotics[J].Current Issues in Intestinal Microbiology,2006,7(2):73-89.

[25] KOCIOLEK L K,GERDING D N.Breakthroughs in the treatment and prevention of Clostridium difficile infection[J].Nature Reviews Gastroenterology & Hepatology,2016,13(3):150-160.  
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2