甘薯渣替代白酒糟对育肥牛肌内脂肪沉积相关基因表达的影响

张海波

doi:10.3969/j.issn.1006-267x.2018.11.045

动物营养学报 >

2018 , Vol. 30 >Issue 11: 4676 - 4682

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.11.045

饲料资源开发 Feed Resources Development

甘薯渣替代白酒糟对育肥牛肌内脂肪沉积相关基因表达的影响

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宜春学院生命科学与资源环境学院, 宜春 336000

张海波(1985-),男,重庆人,讲师,博士研究生,从事反刍动物营养研究。E-mail:zhanghaiboainide@163.com

收稿日期: 2018-05-03

网络出版日期: 2018-11-20

基金资助

江西省教育厅科学技术研究项目（GJJ170919）；宜春学院博士科研启动基金（3350100050）

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Effects of Sweet Potato Residue Replace Distillers' Grains on Intramuscular Fat Deposition Related Genes Expression in Fattening Cattle

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College of Life Science and Environmetal Resource, Yichun University, Yichun 336000, China

Received date: 2018-05-03

Online published: 2018-11-20

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摘要

本试验旨在研究甘薯渣替代白酒糟对育肥牛肌内脂肪（IMF）沉积相关基因表达的影响。选择30头西杂阉公育肥牛（西门塔尔牛×本地黄牛）随机分为3组，每组10头，每个重复1头牛。A组（对照组）饲喂基础饲粮，B组和C组分别用甘薯渣替代基础饲粮中50%和100%的白酒糟，试验期为8周。结果表明：1）C组的平均日增重和IMF含量显著低于A组和B组（P<0.05）。2）C组的血清甘油三酯、总胆固醇、高密度脂蛋白和低密度脂蛋白含量显著低于A组（P<0.05）。3）C组背最长肌脂肪酸合成酶（FAS）和乙酰辅酶A羧化酶（ACC）活性显著低于A组和B组（P<0.05），激素敏感脂肪酶（HSL）和肉碱转移酶-1（CPT-1）活性显著高于A组和B组（P<0.05）。B组背最长肌FAS和ACC活性显著低于A组（P<0.05），HSL和CPT-1活性显著高于A组（P<0.05）。4）C组背最长肌固醇调节元件结合蛋白-1（SREBP-1）、FAS、ACC和过氧化物酶增殖激活受体γ（PPARγ）基因表达量显著低于A组和B组（P<0.05），HSL和CPT-1基因表达量显著高于A组和B组（P<0.05）。B组背最长肌SREBP-1、FAS、ACC和PPARγ基因表达量显著低于A组（P<0.05），HSL和CPT-1基因表达量显著高于A组（P<0.05）。由此可见，提高甘薯渣替代白酒糟比例，可下调育肥牛背最长肌脂肪酸合成相关基因（SREBP-1、FAS、ACC和PPARγ）表达以及上调脂肪分解相关基因（HSL和CPT-1）表达，减少背最长肌IMF沉积。

关键词： 育肥牛; 甘薯渣; 肌内脂肪; 基因表达; 白酒糟

本文引用格式

张海波 . 甘薯渣替代白酒糟对育肥牛肌内脂肪沉积相关基因表达的影响[J]. 动物营养学报, 2018 , 30(11) : 4676 -4682 . DOI: 10.3969/j.issn.1006-267x.2018.11.045

Abstract

This experiment was conducted to study the effects of sweet potato residue replace distillers' grains on the intramuscular fat (IMF) deposition related genes expression in fattening cattle. Thirty crossbred fattening steers (Yellow breed×Simmental cattle) were randomly divided into 3 groups with 10 cattle in each group and 1 cattle in each repetition. Group A (control group) was fed a basal diet, and sweet potato residue was used in groups B and C to replace 50% and 100% distiller's grains on the basal diet. The test lasted for 9 weeks. The results showed as follows:1) the average daily gain and IMF content in group C were significantly lower than those in groups A and B (P<0.05). 2) The serum contents of triglyceride, total cholesterol, high density lipoprotein and low density lipoprotein in group C were significantly lower than those in group A (P<0.05). 3) The activities of fatty acid synthetase (FAS) and acetyl coenzyme A carboxylase activity (ACC) in longissimus dorsi muscle in group C were significantly lower than those in groups A and B (P<0.05), and the activities of hormone sensitive lipase (HSL) and carnitine transferase 1 (CPT-1) were significantly higher than those in groups A and B (P<0.05). The activities of FAS and ACC in longissimus dorsi muscle in group B were significantly lower than those in group A (P<0.05), and the activities of HSL and CPT-1 were significantly higher than those in group A (P<0.05). 4) The gene expression levels of sterol regulator element binding protein-1 (SREBP-1), FAS, ACC, peroxidase activation receptor γ (PPARγ) in longissimus dorsi muscle in group C were significantly lower than those in groups A and B (P<0.05), and the gene expression levels of HSL and CPT-1 were significantly higher than those in groups A and B (P<0.05). The gene expression levels of SREBP-1, FAS, ACC, PPARγ in longissimus dorsi muscle in group B were significantly lower than those in group A (P<0.05), and the gene expression levels of HSL and CPT-1 were significantly higher than those in group A (P<0.05). The results indicate that increasing the replacement ratio of sweet potato residue to distillers' grains can down-regulate the longissimus dorsi muscle fatty acid synthesis related genes (SREBP-1, FAS, ACC and PPARγ) expression, and up-regulate the fatty acid decomposition related genes (HSL and CPT-1) expression, reducing the longissimus dorsi muscle IMF deposition.

Key words： fattening cattle; sweet potato residue; intramuscular fat; gene expression; distiller's grains

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