研究简报 Short communications

纤维素酶和复合益生菌对全株玉米青贮品质的影响

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  • 1. 河南农业大学牧医工程学院, 郑州 450002;
    2. 河南德邻生物制品有限公司, 新乡 453000;
    3. 陕西省微生物研究所, 西安 710043
孙贵宾(1992-),男,河南项城人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:1281687422@qq.com

收稿日期: 2018-04-19

  网络出版日期: 2018-11-20

基金资助

河南省产学研合作计划项目(162107000069);国家自然科学基金项目(U1204325);陕西省科学院项目(2016k-10)

Effects of Cellulase and Compound Probiotics on Silage Quality of Whole Corn

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  • 1. College of Animal Science and Veterinary Medicine, Henan Agriculture University, Zhengzhou 450002, China;
    2. Henan Delin Biological Product Co. Ltd., Xinxiang 453000, China;
    3. Microbiology Institute of Shanxi, Xi'an 710043, China

Received date: 2018-04-19

  Online published: 2018-11-20

摘要

本试验旨在研究纤维素酶和复合益生菌对全株玉米青贮品质的影响。以全株玉米为材料,试验分为对照组(不含添加剂)、纤维素酶组(添加1 g/kg纤维素酶)、复合益生菌组(添加2 mL/kg复合益生菌,植物乳杆菌∶酿酒酵母=1∶1)、菌酶联合组(添加1 g/kg纤维素酶和2 mL/kg复合益生菌),每组3个重复。青贮45 d后,测定全株玉米青贮的营养成分、发酵品质和微生物菌落数,分析纤维素酶和复合益生菌对全株玉米青贮品质的影响。结果表明:1)纤维素酶组和菌酶联合组全株玉米青贮中的粗蛋白质含量均显著高于对照组(P<0.05);与对照组相比,菌酶联合处理显著降低了全株玉米青贮的中性洗涤纤维、酸性洗涤纤维和纤维素的含量(P<0.05)。2)与对照组相比,各试验组全株玉米青贮的感官品质得到提高,可溶性碳水化合物含量和pH显著降低(P<0.05);菌酶联合处理显著降低了全株玉米青贮的氨态氮/总氮(P<0.05)。3)与对照组相比,菌酶联合处理显著降低了全株玉米青贮中的乳酸菌、酵母菌和需氧菌的数量(P<0.05)。本试验得出,纤维素酶和复合益生菌的联合处理有效提高了全株玉米青贮的营养价值和品质。

本文引用格式

孙贵宾, 常娟, 尹清强, 刘超齐, 谢凤莲, 王平, 党晓伟, 张志敏 . 纤维素酶和复合益生菌对全株玉米青贮品质的影响[J]. 动物营养学报, 2018 , 30(11) : 4738 -4745 . DOI: 10.3969/j.issn.1006-267x.2018.11.052

Abstract

The aim of this study was investigated the effects of cellulase and compound probiotics on the silage quality of whole corn. The whole corn was used in this experiment, and was divided into 4 groups:control group (no additive), cellulase group (adding 1 g/kg cellulase), compound probiotics group (adding 2 mL/kg compound probiotics), probiotics and enzyme combination group (adding 1 g/kg cellulase and 2 mL/kg compound probiotics), with 3 replicates in each group. After 45 days of silage, the nutrient composition, fermentation quality and the number of microbial colonies were analyzed. The results showed as follows:1) the crude protein content of cellulase group and probiotics and enzyme combined group were significantly higher than that of control group (P<0.05). The compound probiotics and enzyme combined treatment significantly decreased the neutral detergent fiber, acid detergent fiber and cellulose contents of whole corn silage (P<0.05). 2) The sensory quality of corn silage in each group was improved, and the water soluble carbohydrates content and pH were significantly decreased compared with the control group (P<0.05), and the compound probiotics and enzyme combined treatment significantly decreased the ammonia nitrogen/total nitrogen of whole corn silage (P<0.05). 3) The compound probiotics and enzyme combined treatment significantly decreased the number of lactic acid bacteria, yeasts and aerobic bacteria of whole corn silage (P<0.05). In conclusion, combined treatment of cellulase and compound probiotics can effectively improve the nutritional value and quality of whole corn silage.

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