硒代蛋氨酸对条纹锯生长性能、组织硒含量、抗氧化能力及血清生化指标的影响

张清雯, 陈超, 邵彦翔, 赵亭亭

doi:10.3969/j.issn.1006-267x.2018.11.053

动物营养学报 >

2018 , Vol. 30 >Issue 11: 4746 - 4756

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.11.053

研究简报 Short communications

硒代蛋氨酸对条纹锯生长性能、组织硒含量、抗氧化能力及血清生化指标的影响

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1. 中国水产科学研究院黄海水产研究所, 农业部海洋渔业可持续发展重点实验室, 青岛 266071;
2. 上海海洋大学水产与生命学院, 上海 201306

张清雯(1992-),女,山东诸城人,硕士研究生,从事海水鱼类繁育和营养学研究。E-mail:zhangqingwenzi@126.com

收稿日期: 2018-04-27

网络出版日期: 2018-11-20

基金资助

农业农村部农业国际合作交流项目——“一带一路”热带国家水产养殖科技合作项目

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Effects of Selenomethionine on Growth Performance, Tissue Selenium Content, Antioxidant Capacity and Serum Biochemical Indices of Centropristis striata

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1. Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2. College of Fisheries and Science, Shanghai Ocean University, Shanghai 201306, China

Received date: 2018-04-27

Online published: 2018-11-20

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摘要

本试验旨在研究饲料中添加硒代蛋氨酸（Se-Met）对条纹锯生长性能、组织硒含量、抗氧化能力及血清生化指标的影响。选取初始体重为（81.62±0.75）g的健康条纹锯幼鱼540尾，随机分为6组，每组3个重复，每个重复30尾。各组分别投喂硒含量为0.09、0.21、0.44、0.58、0.77和1.05 mg/kg的试验饲料。试验期8周。结果表明：1）饲料硒含量为0.58 mg/kg组的终末体重和增重率最高，显著高于其他各组（P<0.05）。饲料硒含量为0.58 mg/kg组的饲料系数最低，显著低于饲料硒含量为0.09、0.77和1.05 mg/kg组（P<0.05）。2）饲料硒含量为0.77 mg/kg组的肌肉粗脂肪含量最高，显著高于除饲料硒含量为0.58 mg/kg组的其他各组（P<0.05）。饲料硒含量为0.44 mg/kg组的肌肉粗灰分含量最高，显著高于其他各组（P<0.05）。3）饲料硒含量为0.77 mg/kg组的肝脏中硒含量显著高于饲料硒含量为0.09、0.21和0.44 mg/kg组（P<0.05）。饲料硒含量为0.58 mg/kg组的血清中硒含量最高，显著高于饲料硒含量为0.09、0.21、0.44和1.05 mg/kg组（P<0.05）。4）饲料硒含量为1.05 mg/kg组的血清谷胱甘肽过氧化物酶（GPx）活性显著高于其他各组（P<0.05）。饲料硒含量为0.58 mg/kg组的血清谷胱甘肽还原酶（GR）活性最高，显著高于饲料硒含量为0.77和1.05 mg/kg组。饲料硒含量为0.58 mg/kg组的血清过氧化氢酶（CAT）活性最高，显著高于饲料硒含量为0.09、0.21和1.05 mg/kg组（P<0.05）。饲料硒含量为1.05 mg/kg组的血清谷胱甘肽S-转移酶（GST）活性最高，显著高于饲料硒含量为0.09、0.21、0.44和0.58 mg/kg组（P<0.05）。5）饲料硒含量为0.09 mg/kg组的肝脏GPx活性最低，显著低于饲料硒含量为0.44、0.58和0.77 mg/kg组（P<0.05）。饲料硒含量为0.09 mg/kg组的肝脏GR活性最低，显著低于饲料硒含量为0.44、0.58和1.05 mg/kg组（P<0.05）。饲料硒含量为0.09 mg/kg组的肝脏GST活性最高，显著高于饲料硒含量为0.44、0.58、0.77和1.05 mg/kg组（P<0.05）。饲料硒含量为0.77 mg/kg组的肝脏超氧化物歧化酶（SOD）活性最高，显著高于饲料硒含量为0.09和1.05 mg/kg组（P<0.05）。饲料硒含量为0.44 mg/kg组的肝脏CAT活性最高，显著高于其他各组（P<0.05）。6）饲料硒含量为0.58 mg/kg组的血清总蛋白含量最高，显著高于其他各组（P<0.05）。饲料硒含量为0.58 mg/kg组的血清总胆固醇含量最高，显著高于饲料硒含量为0.09、0.21和0.44 mg/kg组（P<0.05）。饲料硒含量为0.77 mg/kg组的血清免疫球蛋白M含量和溶菌酶活性最高，显著高于饲料硒含量为0.09、0.21、0.44和1.05 mg/kg组（P<0.05）。以增重率、肝脏中硒含量、血清溶菌酶活性为评价指标，通过二次曲线分析得出条纹锯对硒的适宜需求量分别为0.62、0.92、0.72 mg/kg，可见饲料中硒的适宜含量为0.62~0.92 mg/kg。

关键词： 条纹锯; 硒代蛋氨酸; 生长性能; 组织硒含量; 抗氧化能力

本文引用格式

张清雯, 陈超, 邵彦翔, 赵亭亭 . 硒代蛋氨酸对条纹锯生长性能、组织硒含量、抗氧化能力及血清生化指标的影响[J]. 动物营养学报, 2018 , 30(11) : 4746 -4756 . DOI: 10.3969/j.issn.1006-267x.2018.11.053

Abstract

This experiment was conducted to investigate the effects of selenomethionine (Se-Met) on growth performance, tissue selenium content, antioxidant capacity and serum biochemical indices of Centropristis striata. A total of 540 healthy juvenile Centropristis striata with the initial body weight of (81.62±0.75) g were randomly divided into 6 groups with 3 replicates per group and 30 fish per replicate. Fish in those groups were fed experimental diets which containing 0.09 (control group), 0.21, 0.44, 0.58, 0.77 and 1.05 mg/kg selenium, respectively. The experiment lasted for 8 weeks. The results showed as follows:1) the final body weight and weight gain rate of dietary selenium content 0.58 mg/kg group were the highest, and significantly higher than those of other groups (P<0.05). The feed conversion rate of dietary selenium content 0.58 mg/kg group was the lowest, and significantly lower than that of dietary selenium content 0.09, 0.77 and 1.05 mg/kg groups (P<0.05). 2) The muscle crude lipid content of dietary selenium content 0.77 mg/kg group was the highest, and significantly higher than that of other groups except dietary selenium content 0.58 mg/kg group (P<0.05). The muscle crude ash content of dietary selenium content 0.44 mg/kg group was the highest, and significantly higher than that of other groups (P<0.05). 3) The liver selenium content of dietary selenium content 0.77 mg/kg group was significantly higher than that of dietary selenium content 0.09, 0.21 and 0.44 mg/kg groups (P<0.05). The serum selenium content of dietary selenium content 0.58 mg/kg group was the highest, and significantly higher than that of dietary selenium content 0.09, 0.21, 0.44 and 1.05 mg/kg groups (P<0.05). 4) The serum glutathione peroxidase (GPx) activity of dietary selenium content 1.05 mg/kg group was significantly higher than that of other groups (P<0.05). The serum glutathione reductase (GR) activity of dietary selenium content 0.58 mg/kg group was the highest, and significantly higher than that of dietary selenium content 0.77 and 1.05 mg/kg groups (P<0.05). The serum catalase (CAT) activity of dietary selenium content 0.58 mg/kg group was the highest, and significantly higher than that of dietary selenium content 0.09, 0.21 and 1.05 mg/kg groups (P<0.05). The serum glutathione S-transferase (GST) activity of dietary selenium content 1.05 mg/kg group was the highest, and significantly higher than that of dietary selenium content 0.09, 0.21, 0.44 and 0.58 mg/kg groups (P<0.05). 5) The liver GPx activity of dietary selenium content 0.09 mg/kg group was the lowest, and significantly lower than that of 0.44, 0.58 and 0.77 mg/kg groups (P<0.05). The liver GR activity of dietary selenium content 0.09 mg/kg group was the lowest, and significantly lower than that of 0.44, 0.58 and 1.05 mg/kg groups (P<0.05). The liver GST activity of dietary selenium content 0.09 mg/kg group was the highest, and significantly higher than that of dietary selenium content 0.44, 0.58, 0.77 and 1.05 mg/kg groups (P<0.05). The liver superoxide dismutase (SOD) activity of dietary selenium content 0.77 mg/kg group was the highest, and significantly higher than that of dietary selenium content 0.09 and 1.05 mg/kg groups (P<0.05). The liver CAT activity of dietary selenium content 0.44 mg/kg group was the highest, and significantly higher than that of other groups (P<0.05). 6) The serum total protein content of dietary selenium content 0.58 mg/kg group was the highest, and significantly higher than that of other groups (P<0.05). The serum total cholesterol content of dietary selenium content 0.58 mg/kg group was the highest, and significantly higher than that of dietary selenium content 0.09, 0.21 and 0.44 mg/kg groups (P<0.05). The serum immunoglobulin M content and lysozyme activity of dietary selenium content 0.77 mg/kg group was the highest, and significantly higher than those of dietary selenium content 0.09, 0.21, 0.44 and 1.05 mg/kg groups (P<0.05). Base on weight gain rate, liver GPX activity and serum lysozyme activity as evaluating indicators by quadratic curve analysis, the dietary selenium requirement of Centropristis striata is 0.62, 0.92 and 0.72 mg/kg, respectively, and the suitable dietary selenium content is 0.62 to 0.92 mg/kg.

Key words： Centropristis striata; selenomethionine; growth performance; tissue selenium content; antioxidant capacity

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