禽营养 Poultry nutrition

蛋氨酸缺乏对肉仔鸡小肠发育和杯状细胞的影响

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  • 1. 黔东南民族职业技术学院, 凯里 556000;
    2. 西华师范大学生命科学学院, 西南野生动植物资源保护教育部重点实验室, 南充 637009
刘晓东(1984-),男,四川达州人,副教授,硕士,主要从事动物病理学的研究。E-mail:liuxiaodongk@163.com

收稿日期: 2018-05-18

  网络出版日期: 2018-12-19

Effects of Methionine Deficiency on Small Intestinal Development and Goblet Cells of Broilers

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  • 1. Qiandongnan Vocational & Technical College for Nationalities, Kaili 556000, China;
    2. Key Laboratory of Southwest China Wildlife Resources Conservation of Ministry of Education, College of Life Science, China West Normal University, Nanchong 637009, China

Received date: 2018-05-18

  Online published: 2018-12-19

摘要

本研究旨在探讨蛋氨酸缺乏对肉仔鸡小肠发育和杯状细胞的影响。选用120只1日龄的健康雏鸡[体重为(45±5) g],随机分为2组(每组6个重复,每个重复10只鸡),分别饲喂基础饲粮(对照组)和蛋氨酸缺乏饲粮(试验组),试验期42 d,于第14、28和42天采集样品,利用光学显微镜与组织化学方法测定小肠各段绒毛高度、隐窝深度以及杯状细胞数量。结果表明:与对照组相比,试验组肉仔鸡小肠各肠段绒毛高度、隐窝深度以及绒毛高度与隐窝深度的比值显著或极显著降低(P<0.05或P<0.01),小肠杯状细胞数量也显著或极显著减少(P<0.05或P<0.01)。结果提示,蛋氨酸缺乏会导致小肠绒毛高度、隐窝深度降低以及杯状细胞数量减少,从而导致肉仔鸡小肠正常发育受阻以及功能受损。

本文引用格式

刘晓东, 彭利兰, 何胜梅, 张森, 莘海亮, 吴邦元 . 蛋氨酸缺乏对肉仔鸡小肠发育和杯状细胞的影响[J]. 动物营养学报, 2018 , 30(12) : 4898 -4906 . DOI: 10.3969/j.issn.1006-267x.2018.12.017

Abstract

Aims of this research were to determine the effects of methionine (Met) deficiency on the small intestine development and goblet cells of broilers. A total of 120 one-day-old broilers with body weight of (45±5) g were divided into two groups with 6 replicates each group and 10 broilers in each replicate. Broilers in the control group were fed a basal diet, and those in the experimental group were fed Met-deficient diet for 42 days. Samples were collected on day 14, 28 and 42 and the villus height, crypt depth and the goblet cell population in different small intestine sections of broilers were detected by the light microscopy and histochemistry method. Results showed that the small intestinal villus height, crypt depth and the ratio of villus height to crypt depth were significantly or extremely significantly decreased (P<0.05 or P<0.01), and the small intestinal goblet cell amounts were also significantly or extremely significantly decreased (P<0.05 or P<0.01) in the experimental group compared with the control group. In conclusion, Met deficiency can reduce the villus height, crypt depth and the goblet cell population, indicating that the normal development and function of small intestine of broilers are finally impaired.

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