动物营养学报 >

2018 , Vol. 30 >Issue 12: 4939 - 4946

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.12.021

禽营养 Poultry nutrition

羟基蛋氨酸锌对产蛋后期蛋鸡生产性能、蛋品质和免疫相关基因表达的影响

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  • 西北农林科技大学动物科技学院, 杨凌 712100
齐茜(1994-),女,陕西周至人,硕士研究生,动物营养与饲料科学专业。E-mail:1336762544@qq.com

收稿日期: 2018-05-28

  网络出版日期: 2018-12-19

基金资助

现代农业产业技术体系建设专项资金（CARS-40-S20）；陕西省科技统筹创新工程计划项目（2016KTL02-18）

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Effects of Methionine Hydroxy Analog Chelate Zinc on Performance, Eggshell Quality and Immune-Related Gene Expression of Laying Hens during Later Period of Laying

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  • College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China

Received date: 2018-05-28

  Online published: 2018-12-19

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摘要

本试验旨在研究羟基蛋氨酸锌（MHA-Zn）对产蛋后期蛋鸡生产性能、蛋品质和免疫相关基因表达的影响。选取体重和产蛋率相近的57周龄海兰灰蛋鸡960只，随机分为4个组，每组8个重复，每个重复30只。参照NRC（1994）蛋鸡饲养标准和海兰公司推荐的饲粮营养水平配制玉米-豆粕型基础饲粮（锌含量35.08 mg/kg），对照组在基础饲粮中添加80 mg/kg硫酸锌（以锌计），试验组分别在基础饲粮中添加20、40和80 mg/kg MHA-Zn（以锌计）。预试期4周，正试期12周。结果表明：1）各组之间蛋鸡的产蛋率、平均蛋重、平均日采食量、料蛋比、日产蛋重均无显著差异（P>0.05）。2）各组之间鸡蛋的蛋白高度、哈夫单位和蛋黄颜色均无显著差异（P>0.05）。40和80 mg/kg MHA-Zn组鸡蛋的蛋壳厚度和蛋壳强度均显著高于对照组和20 mg/kg MHA-Zn组（P<0.05），破蛋率显著低于对照组和20 mg/kg MHA-Zn组（P<0.05）。3）各组之间蛋鸡的脾脏免疫相关基因核因子-κB1（NF-κB1）、肿瘤坏死因子-α（TNF-α）和白细胞介素-10（IL-10）mRNA的相对表达量均无显著差异（P>0.05）。80 mg/kg MHA-Zn组的脾脏白细胞介素-8（IL-8）mRNA的相对表达量显著低于40 mg/kg MHA-Zn组（P<0.05），40 mg/kg MHA-Zn组显著低于20 mg/kg MHA-Zn组（P<0.05）。综上所述，饲粮中添加40和80 mg/kg的MHA-Zn可显著提高蛋壳厚度和蛋壳强度，显著降低破蛋率，并显著降低促炎因子IL-8 mRNA的相对表达量。建议产蛋后期蛋鸡饲粮中MHA-Zn适宜添加水平为40 mg/kg。

关键词： 蛋鸡; 羟基蛋氨酸锌; 生产性能; 蛋品质; 免疫相关基因

本文引用格式

齐茜, 李佶隆, 马淑雪, 刘幸, 高玉鹏, 闵育娜 . 羟基蛋氨酸锌对产蛋后期蛋鸡生产性能、蛋品质和免疫相关基因表达的影响[J]. 动物营养学报, 2018 , 30(12) : 4939 -4946 . DOI: 10.3969/j.issn.1006-267x.2018.12.021

Abstract

This experiment was conducted to study the effects of methionine hydroxy analog chelate zinc (MHA-Zn) on performance, eggshell quality and immune-related gene expression of laying hens during later period of laying. A total of 960 Hy-Line grey laying hens (57-week-old) with similar body weight and laying rate were randomly assigned into 4 groups with 8 replicates per group and 30 hens per replicate. The corn-soybean meal basal diet (zinc content was 35.08 mg/kg) was formulated according to NRC(1994) standard for laying hens and the recommended nutrient levels of Hy-Line company, hens in the control group were fed the basal diet supplemented with 80 mg/kg zinc sulfate (as zinc), and hens in the experimental groups were fed the basal diet supplemented with 20, 40 and 80 mg/kg MHA-Zn (as zinc), respectively. The pre-experimental period lasted for 4 weeks, and the experimental period lasted for 12 weeks. The results showed as follows: 1) there were no significant differences in laying rate, average egg weight, average daily feed intake, feed to egg ratio and daily egg mass of laying hens among all groups (P>0.05). 2) There were no significant differences in albumen height, Haugh unit and yolk color of eggs among all groups (P>0.05). The eggshell strength and eggshell thickness of eggs in 40 and 80 mg/kg MHA-Zn groups were significantly higher than those in control group and 20 mg/kg MHA-Zn group (P<0.05), and the broken egg rate was significantly lower than that in control group and 20 mg/kg MHA-Zn group (P<0.05). 3) There were no significant differences in the mRNA relative expression levels of immune-related genes such as nuclear factor-κB1 (NF-κB1), tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) in spleen among all groups (P>0.05). The mRNA relative expression level of interleukin-8 (IL-8) in spleen in 80 mg/kg MHA-Zn group was significantly lower than that in 40 mg/kg MHA-Zn group (P<0.05), and the mRNA relative expression level of IL-8 in spleen in 40 mg/kg MHA-Zn group was significantly lower than that in 20 mg/kg MHA-Zn group (P<0.05). In conclusion, dietary supplemented with 40 and 80 mg/kg MHA-Zn can significantly increase the eggshell thickness and eggshell strength, significantly decrease the broken egg rate, and significantly decrease the mRNA relative expression level of proinflammatory factor IL-8. The optimal dietary MHA-Zn level of laying hens during later period of laying is 40 mg/kg.

Key words： laying hens; MHA-Zn; performance; eggshell quality; immune-related gene

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