条纹锯鮨幼鱼维生素C需要量

赵亭亭, 陈超, 邵彦翔, 张清雯, 刘玲

doi:10.3969/j.issn.1006-267x.2018.12.034

动物营养学报 >

2018 , Vol. 30 >Issue 12: 5062 - 5074

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.12.034

水产营养 Aquaculture Nutrition

条纹锯鮨幼鱼维生素C需要量

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1. 中国水产科学研究院黄海水产研究所, 青岛 266071;
2. 上海海洋大学水产与生命学院, 上海 201306

赵亭亭(1992-),女,山东济南人,硕士研究生,从事鱼类营养与饲料研究。E-mail:2467832293@qq.com

收稿日期: 2018-05-28

网络出版日期: 2018-12-19

基金资助

中国-东盟海上合作基金（2016—2018）；农业农村部农业国际合作交流项目—热带国家水产养殖科技创新合作；"海上丝路"国家鱼类养殖关键技术研究

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Vitamin C Requirement of Juvenile Centropristis striata

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1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao 266071, China;
2. College of Fishers and Life Science, Shanghai Ocean University, Shanghai 201306, China

Received date: 2018-05-28

Online published: 2018-12-19

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摘要

本试验旨在研究条纹锯鮨（Centropristis striata）幼鱼维生素C需要量。试验采用单因素试验设计法，选取平均体重为（88.34±24.10）g的条纹锯鮨幼鱼630尾，随机分为7组，每组3个重复，每个重复30尾鱼。各组分别投喂维生素C含量（实测值）分别为10.97（对照）、74.03、148.33、213.64、335.33、618.88和910.98 mg/kg的等氮等能饲料。检测饲料维生素C含量对条纹锯鮨幼鱼生长性能、血清生化指标、相关酶活性、各组织中维生素C积累量和肌肉成分的影响。试验期8周。结果表明：1）74.03 mg/kg组的增重率和特定生长率显著高于10.97、618.88和910.98 mg/kg组（P<0.05），74.03 mg/kg组的饲料系数显著低于10.97和910.98 mg/kg组（P<0.05），10.97和910.98 mg/kg组蛋白质效率显著低于74.03和618.88 mg/kg组（P<0.05），335.33 mg/kg组的肥满度显著低于74.03、148.33和213.64 mg/kg组（P<0.05）。2）10.97、74.03 mg/kg组血清总蛋白含量显著低于其他各组（P<0.05），618.88和910.98 mg/kg组血清甘油三酯含量显著低于其他各组（P<0.05），10.97 mg/kg组血清溶菌酶活性显著低于213.64、335.33、618.88、910.98 mg/kg组（P<0.05）。3）335.33 mg/kg组血清超氧化物歧化酶（SOD）活性显著高于10.97、74.03、910.98 mg/kg组（P<0.05），335.33 mg/kg组血清总抗氧化能力（T-AOC）显著高于10.97、618.88、910.98 mg/kg组（P<0.05），335.33 mg/kg组血清丙二醛（MDA）含量显著低于10.97 mg/kg组（P<0.05）。4）10.97 mg/kg组肝脏SOD和过氧化物酶（CAT）活性显著低于其他各组（P<0.05），148.33、335.33和910.98 mg/kg组肝脏MDA含量显著低于10.97 mg/kg（P<0.05）。5）74.03 mg/kg组肌肉SOD活性和T-AOC均显著高于其他各组（P<0.05）。6）213.64、335.33、618.88、910.98 mg/kg组血清谷丙转氨酶（GOT）活性显著低于10.97 mg/kg组（P<0.05），10.97 mg/kg组肝脏GOT活性显著低于其他各组（P<0.05）。10.97、74.03 mg/kg组血清谷草转氨酶（GPT）活性显著高于其他各组（P<0.05）。7）910.98 mg/kg组血清中维生素C积累量显著高于其他各组（P<0.05），910.98 mg/kg组肝脏中维生素C积累量显著高于10.97、74.03、148.33和213.64 mg/kg组（P<0.05），618.88 mg/kg组的肌肉中维生素C积累量显著高于10.97、74.03、148.33和213.64 mg/kg组（P<0.05）。8）74.03 mg/kg组肌肉水分含量显著低于10.97、148.33和213.64 mg/kg组（P<0.05），910.98 mg/kg组肌肉胶原蛋白含量显著高于其他各组（P<0.05）。由此可见，以特定生长率、肝脏中维生素C积累量和血清溶菌酶活性为评价标准，通过折线回归模型得出条纹锯鮨幼鱼饲料维生素C需要量分别为66.66、309.93和345.11 mg/kg。

关键词： 条纹锯鮨; 维生素C; 生长; 抗氧化; 维生素C积累量

本文引用格式

赵亭亭, 陈超, 邵彦翔, 张清雯, 刘玲 . 条纹锯鮨幼鱼维生素C需要量[J]. 动物营养学报, 2018 , 30(12) : 5062 -5074 . DOI: 10.3969/j.issn.1006-267x.2018.12.034

Abstract

This experiment was conducted to investigate the vitamin C requirement of juvenile Centropristis striata. A single factor test design was adopted in the experiment, a total of 630 juvenile Centropristis striata with average body weight of (88.34±24.10) g were selected and randomly divided into 7 groups with 3 replicates per group and 30 fishes per replicate. Fishes in the 7 groups were fed the isonitrogenous and isocaloric diets which vitamin C contents (measured values) were 10.97 (control), 74.03, 148.33, 213.64, 335.33, 618.88, 910.98 mg/kg, respectively. The effects of dietary vitamin C on growth performance, serum biochemical indices, related enzyme activities, tissue vitamin C concentrations and muscle composition of juvenile Centropristis striata were detected. The experiment lasted for 8 weeks. The results showed as follows: 1) the weight gain rate and special gain rate in 74.03 mg/kg group were significantly higher than those in 10.97, 618.88 and 910.98 mg/kg groups (P<0.05), the feed conversion ratio in 74.03 mg/kg group was significantly lower than that in 10.97 and 910.98 mg/kg groups (P<0.05), the protein efficiency ratio in 10.97 and 910.98 mg/kg groups was significantly lower than that in 74.03 and 618.88 mg/kg groups (P<0.05), the condition factor in 335.33 mg/kg group was significantly lower than that in 74.03, 148.33 and 213.64 mg/kg groups (P<0.05). 2) The serum total protein content in 10.97 and 74.03 mg/kg groups was significantly lower than that in other groups (P<0.05), the serum triglyceride content in 618.88 and 910.98 mg/kg groups was significantly lower than that in other groups (P<0.05), the serum lysozyme activity in 10.97 mg/kg group was significantly lower than that in 213.64, 335.33, 618.88 and 910.98 mg/kg groups (P<0.05). 3) The serum superoxide dismutase (SOD) activity in 335.33 mg/kg group was significantly higher than that in 10.97, 74.03 and 910.98 mg/kg groups (P<0.05), the serum total antioxidant capacity (T-AOC) in 335.33 mg/kg group was significantly higher than that in 10.97, 618.88 and 910.98 mg/kg groups (P<0.05), the serum malondialdehyde (MDA) content in 335.33 mg/kg group was significantly lower than that in 910.98 mg/kg group (P<0.05). 4) The activities of SOD and catalase (CAT) in liver in 10.97 mg/kg group were significantly lower than those in other groups (P<0.05), the liver MDA content in 148.33, 335.33 and 910.98 mg/kg was significantly lower than that in 10.97 mg/kg group (P<0.05). 5) The muscle SOD activity and T-AOC in 74.03 mg/kg group were significantly higher than those in other groups (P<0.05). 6) The serum glutamic oxalacetic transaminase (GOT) activity in 213.64, 335.33, 618.88 and 910.98 mg/kg groups was significantly lower than that in 10.97 mg/kg group (P<0.05), the liver GOT activity in 10.97 mg/kg group was significantly lower than that in other groups (P<0.05). The serum glutamic-pyruvic transaminase (GPT) activity in 10.97 and 74.03 mg/kg groups was significantly higher than that in other groups (P<0.05). 7) The serum vitamin C accumulation in 910.98 mg/kg group was significantly higher than that in other groups (P<0.05), the liver vitamin C accumulation in 910.98 mg/kg group was significantly higher than that in 10.97, 74.03, 148.33 and 213.64 mg/kg groups (P<0.05), the muscle vitamin C accumulation in 618.88 mg/kg group was significantly higher than that in 10.97, 74.03, 148.33 and 213.64 mg/kg groups (P<0.05). 8) The muscle moisture content in 74.03 mg/kg group was significantly lower than that in 10.97, 148.33 and 213.64 groups (P<0.05), the muscle collagen content in 910.98 mg/kg group was significantly higher than that in other groups (P<0.05). In conclusion, set the special gain rate, liver vitamin C accumulation and serum lysozyme activity as evaluation indexes, the broken-line regression analysis results indicate that dietary vitamin C requirements of juvenile Centropristis striata are 66.66, 309.93 and 345.11 mg/kg, respectively.

Key words： Centropristis striata; vitamin C; growth; antioxidant; vitamin C accumulation

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