分子营养 Molecular Nutrition

牛膝多糖抑制沙门氏菌侵染猪小肠上皮细胞的研究

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  • 湖南农业大学动物科技学院, 湖南畜禽安全生产协同创新中心, 长沙 410128
赵玉蓉(1971-),女,湖南澧县人,教授,博士,从事动物营养与饲料研究。E-mail:1335434506@qq.com

收稿日期: 2018-05-24

  网络出版日期: 2018-12-19

基金资助

湖南省自然科学基金(10JJ5015)

Study on Inhibitory Effects of Achyranthes bidentata Polysaccharides on Salmonella Infection in Intestinal Porcine Epithelial Cells-1

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  • Hunan Co-Innovation Center of Animal Production Safety, College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China

Received date: 2018-05-24

  Online published: 2018-12-19

摘要

本试验以猪小肠上皮细胞(IPEC-1)为模型,探讨牛膝多糖(ABPS)对IPEC-1增殖、紧密连接相关蛋白mRNA表达和沙门氏菌侵染的影响。在IPEC-1细胞培养基中分别加入相应剂量的ABPS,使培养基中ABPS终浓度分别为0(对照)、50、100、200、400 μg/mL。分别采用噻唑蓝(MTT)法、实时荧光定量PCR法和平板细菌计数法检测ABPS对IPEC-1的影响。结果表明:1)与对照组相比,ABPS对IEPC-1增殖无显著影响(P>0.05)。2)ABPS处理能显著抑制沙门氏菌的侵染,随着ABPS浓度的增加,其抑制效果先增加后减少,浓度为50 μg/mL时抑制效果达到峰值,其细胞被侵染的沙门氏菌数量极显著低于对照组和200、400 μg/mL ABPS组(P<0.01)。3)与对照组相比,50、200 μg/mL ABPS均极显著上调IEPC-1紧密连接相关蛋白[Ras相关的C3肉毒素底物1(RAC1)、细胞质密闭小带-1(ZO-1)、闭锁蛋白(occludin)、闭合蛋白-1(claudin-1)]mRNA的相对表达量(P<0.01)。由此可知,适量的ABPS能够通过上调IEPC-1紧密连接相关蛋白mRNA表达,从而增强小肠黏膜的屏障功能,抑制沙门氏菌的侵染。

本文引用格式

赵玉蓉, 王耀东 . 牛膝多糖抑制沙门氏菌侵染猪小肠上皮细胞的研究[J]. 动物营养学报, 2018 , 30(12) : 5083 -5088 . DOI: 10.3969/j.issn.1006-267x.2018.12.036

Abstract

This experiment was conducted to evaluate the effects of Achyranthes bidentata polysaccharides (ABPS) on proliferation, mRNA expression of tight junction related proteins and infection of Salmonella in intestinal porcine epithelial cells-1 (IPEC-1). Adding ABPS in IPEC-1 culture medium, the final concentration of ABPS were 0 (control), 50, 100, 200, 400 μg/mL. The effects of ABPS on IPEC-1 were detected using MTT assay, quantitative real-time PCR and plate colony counting method. The results showed as follows: 1) compared with the control group, ABPS had no significant effect on IPEC-1 proliferation (P>0.05). 2) The ABPS significantly inhibited Salmonella infection in IPEC-1, with the increase of ABPS concentration, the number of infected Salmonella decreased first and then increased. The number of infected salmonella in IPEC-1 treated with 50 μg/mL ABPS was the lowest among all groups, and was significantly lower than that in control group and 200 and 400 μg/mL of ABPS groups (P<0.01). 3) Compared with the control group, 50 and 200 μg/mL of ABPS significantly up-regulated the mRNA relative expression level of tight junction related proteins (ras-related C3 botulinum toxin substrate 1, zonula occluden-1, occludin, claudin-1) in IEPC-1 (P<0.01). Above results indicate that suitable ABPS concentration can promote IEPC-1 proliferation, strengthen the barrier function of intestinal mucosa and inhibit Salmonella infection with up-regulation the mRNA expression of tight junction related proteins.

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