动物营养学报 >

2018 , Vol. 30 >Issue 12: 5153 - 5163

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.12.044

饲料营养 Feed science and technology

壳寡糖对异育银鲫生长性能、肠道组织结构和非特异性免疫功能的影响

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  • 1. 苏州大学基础医学与生物科学学院, 江苏省水产动物营养重点实验室, 苏州 215123;
    2. 中泰和(北京)科技发展有限公司, 北京 100027
孙飞(1993-),男,河南鹤壁人,硕士研究生,研究方向为水产动物营养与饲料。E-mail:1152956552@qq.com

收稿日期: 2018-05-28

  网络出版日期: 2018-12-19

基金资助

水产动物功能性糖的营养与饲料应用研究（P113401216）

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Effects of Chitosan Oligosaccharide on Growth Performance, Intestinal Structure and Non-Specific Immune Function of Crucian Carp (Carassius auratus gibelio)

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  • 1. Key Laboratory of Aquatic Nutrition of Jiangsu Province, School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China;
    2. Zhongtaihe(Beijing) Science & Technology Development Company, Beijing 100027, China

Received date: 2018-05-28

  Online published: 2018-12-19

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摘要

本试验旨在研究壳寡糖（COS）对异育银鲫生长性能、肠道组织结构及非特异性免疫功能的影响。在含正常豆油、氧化豆油的基础饲料中分别添加0、0.02%、0.04%、0.06%的COS，共配制成8种试验饲料（CG、OO、CG-200、CG-400、CG-600、OO-200、OO-400、OO-600）。其中，以油脂原料为豆油的未添加COS的饲料（CG）作为正对照组，以油脂原料为氧化豆油的未添加COS的饲料（OO）作为负对照组。将初始体重为（7.60±0.05） g的960尾异育银鲫随机分成8组，每组设3个重复（网箱），每个重复40尾鱼，在池塘网箱中养殖72 d。结果显示：与CG组相比，CG-200组的特定生长率（SGR）升高了16.20%（P<0.05），CG-400和CG-600组则无显著变化（P>0.05）；CG-200组肠皱襞高度增加了86.84%（P<0.05），肠壁厚度增加了20.45%（P<0.05），肠皱襞宽度减少了12.18%（P>0.05），血清超氧化物歧化酶（SOD）活性上升了6.59%（P>0.05），血清丙二醛（MDA）含量下降了16.94%（P>0.05）；OO组SGR下降了8.10%（P<0.05），肠皱襞高度降低了17.81%（P<0.05），肠皱襞宽度增加了70.37%（P<0.05），肠壁厚度减少了30.33%（P>0.05），血清SOD活性下降了27.35%（P<0.05），血清MDA含量上升了25.72%（P<0.05）。与OO组比，OO-200、OO-400、OO-600组SGR升高不显著（P>0.05），其中OO-400组肠皱襞高度增加了18.89%（P<0.05），肠皱襞宽度降低了42.99%（P<0.05），肠壁厚度增加了38.35%（P>0.05），血清SOD活性上升了40.90%（P<0.05），血清MDA含量下降了16.20%（P>0.05）。OO-400组在SGR、肠道结构、血清SOD活性及MDA含量等指标上达到了与CG组相同的水平。本试验条件下，根据结果得出：在含4%豆油的常规饲料中添加0.02%的COS可以促进异育银鲫生长，改善肠道结构，提高鱼体非特异性免疫功能；氧化豆油会对异育鲫鱼的生长和健康造成负面影响，在该饲料中添加0.04%的COS可改善由氧化豆油引起的负面影响，使异育银鲫的健康程度达到正常水平。

关键词： 异育银鲫; 壳寡糖; 氧化油脂; 肠道组织结构; 非特异性免疫指标

本文引用格式

孙飞, 何杰, 叶元土, 蔡春芳, 吴萍, 吴代武, 周露阳, 高敏敏, 郁浓, 张艳芳 . 壳寡糖对异育银鲫生长性能、肠道组织结构和非特异性免疫功能的影响[J]. 动物营养学报, 2018 , 30(12) : 5153 -5163 . DOI: 10.3969/j.issn.1006-267x.2018.12.044

Abstract

In order to study the effects of chitosan oligosaccharides (COS) on growth performance, intestinal structure and non-specific immune function of crucian carp (Carassius auratus gibelio), eight experimental diets were formulated which added 0, 0.02%, 0.04% and 0.06% COS into basal diets containing normal soybean oil or oxidized soybean oil, respectively, and those diets were named as CG, OO, CG-200, CG-400, CG-600, OO-200, OO-400 and OO-600, respectively. The diet which did not add COS and take normal soybean oil as oil material (CG) was set as a positive control group, and the diet which did not add COS and take oxidized soybean oil as oil material (OO) was set as a negative control group. A total of 960 crucian carp with an initial body weight of (7.60±0.05) g were randomly divided into 8 groups with 3 replicates (net cages) per group and 40 fish per replicate, and cultured in a pond net cage for 72 d. The results showed that, compared with CG group, the specific growth rate (SGR) of CG-200 group was increased by 16.20% (P<0.05), but that of CG-400 and CG-600 groups had no significant change (P>0.05); the intestinal fold height and intestinal wall thickness were increased by 86.84% and 20.45% (P<0.05), the intestinal fold width was decreased by 12.18% (P>0.05), the serum superoxide dismutase (SOD) activity was increased by 6.59% (P>0.05) and the serum malondialdehyde (MDA) content was decreased by 16.94% (P>0.05) of CG-200 group; the SGR was decreased by 8.10% (P<0.05), the intestinal fold height was decreased by 17.81%, the intestinal fold width was increased by 70.30% (P<0.05), the intestinal wall thickness was decreased by 30.33% (P>0.05), the serum SOD activity was decreased 27.35% (P<0.05) and the serum MDA content was increased by 25.72% (P<0.05) of OO group. Compared with the OO group, the SGR of OO-200, OO-400 and OO-600 groups was tend to increase (P>0.05). The intestinal fold height was increased by 18.89% (P<0.05), the intestinal fold width was decreased by 42.99% (P<0.05), the intestinal wall thickness was decreased by 38.35% (P>0.05), the serum SOD activity was increased by 40.90% (P<0.05) and the serum MDA content was decreased 16.20% (P>0.05) of OO-400 group. The fish of OO-400 group achieved the similar states in SGR, intestinal structure, serum SOD activity and MDA content as those of CG group. In this experiment, the results indicate that, the growth, intestinal structure and non-specific immunity of crucian carp can be improved by adding 0.02% COS to a 4% soybean oil routine diet. Oxidized oil in the diet has negative effects on the growth and health of crucian carp. Adding 0.04% COS to a 4% oxidized soybean oil diet can improve the negative effects which caused by oxidizing oil, and can adjust the health degree of crucian carp to the normal level.

Key words： crucian carp (Carassius auratus gibelio); chitosan oligosaccharide; oxidized oil; intestinal structure; non-specific immune indexes

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