本试验旨在研究L-精氨酸（L-Arg）对冷应激仔猪生长性能、免疫功能及肝脏、肾脏中肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）基因表达量的影响。试验采用双因素有重复完全交叉析因试验设计，选择26日龄断奶、体重[（7.48±0.47）kg]相近的"杜×长×大"三元杂交仔猪72头，按体重和性别完全随机分为6组，每组6个重复，每个重复2头猪，分别接受2个温度（适温23~27℃、低温14~18℃）和3个L-Arg添加量（0、0.8%、1.2%）的交叉处理。试验期21 d。结果显示：1）温度（P=0.006）和L-Arg（P=0.027）对仔猪平均日增重有显著影响，二者交互作用能显著提高仔猪平均日增重（P=0.047）；温度和L-Arg及二者交互作用对仔猪平均日采食量均没有显著影响（P>0.05）；温度（P=0.031）和L-Arg（P=0.037）均对料重比有显著影响，二者交互作用对料重比的影响不显著（P=0.269）。2）温度和L-Arg对仔猪胸腺重量和脾脏重量均有显著影响（P<0.05），二者交互作用对胸腺重量的影响不显著（P=0.352），但对脾脏重量有显著影响（P=0.026）。3）温度和L-Arg的交互作用对血清免疫球蛋白（Ig）A（P=0.049）、IgM（P=0.021）和IgG（P=0.015）含量有显著影响。4）温度和L-Arg的交互作用对肝脏中IFN-γ基因表达量有显著影响（P=0.018），对肾脏中TNF-α和IFN-γ基因表达量无显著影响（P>0.05）。结果提示，饲粮中添加适量L-Arg可以改善冷应激仔猪的生长性能，提高其机体免疫功能。

The aim of this experiment was to investigate the effects of L-arginine (L-Arg) on growth performance, immune function and gene expression levels of tumor necrosis factor-α(TNF-α) and interferon-γ (IFN-γ) in the liver and kidney of cold stressed piglets. The experiment was designed with two factors with repeated complete cross factorial test. According to body weight and sexual distinction, a total of 72 piglets with 28 days of weaning and similar weight[(7.48±0.47) kg] were selected and divided into 6 groups with 6 repetitions per group and 2 piglets per repetition, and cross treated with two temperatures (normal temperature 23 to 27℃, low temperature 14 to 18℃) and three levels of L-Arg supplementation (0, 0.8% and 1.2%). The experiment lasted for 21 d. The results showed as follows:1) temperature (P=0.006) and L-Arg (P=0.027) had significant influence on the average daily gain of piglets, and their interaction could significantly increase the average daily gain of piglets (P=0.047). The temperature, L-Arg and their interaction had no significant influence on the average daily feed intake of piglets (P>0.05). Temperature (P=0.031) and L-Arg (P=0.037) had significant effects on the ratio of feed to gain, and their interaction had no significant effect on the ratio of feed to gain (P=0.269). 2) Temperature and L-Arg had significant influence on the weight of thymus and spleen of piglets (P<0.05), and their interaction had no significant effect on thymus weight (P=0.352), while significantly affected the weight of spleen (P=0.026). 3) The interaction of temperature and L-Arg had significant effects on serum immunoglobulin (Ig) A (P=0.049), IgM (P=0.021) and IgG (P=0.015) contents. 4) The interaction of temperature and L-Arg had significant effects on the gene expression level of IFN-γ in the liver (P=0.018), and had no significant effect on the gene expression levels of TNF-α and IFN-γ in the kidney (P>0.05). The results show that adding L-Arg to the diet can improve the growth performance and immune function of the cold-stressed piglets.