动物营养学报 >

2019 , Vol. 31 >Issue 1: 140 - 147

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.01.019

猪与禽营养 Swine and poultry nutrition

高钼饲粮对产蛋后期蛋鸡生产性能、蛋品质、血清生化指标和肝脏抗氧化指标的影响

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  • 四川农业大学动物营养研究所, 动物抗病营养教育部重点实验室, 动物抗病营养与饲料农业部重点实验室, 成都 611130
王建萍(1983-),女,四川成都人,副研究员,博士,从事家禽营养和蛋品安全与质量调控研究。E-mail:wangjianping@sicau.edu.cn

收稿日期: 2018-06-29

  网络出版日期: 2019-01-16

基金资助

国家科技支撑计划（2014BAD13B04）；四川省蛋鸡产业链项目（2018NZ0009，2014NZ0002，2014NZ0043，2013NZ0054）；四川省科创饲料产业技术研究院（2013NZ0054）

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Effects of High Molybdenum Diet on Performance, Egg Quality, Serum Biochemical Indexes and Hepatic Antioxidative Indexes of Laying Hens during Late Laying Period

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  • Animal Nutrition Institute, Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Key Laboratory for Animal Disease-Resistance Nutrition and Feed, Ministry of Agriculture, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2018-06-29

  Online published: 2019-01-16

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摘要

本试验旨在研究高钼饲粮对产蛋后期蛋鸡生产性能、蛋品质、血清生化指标和肝脏抗氧化指标的影响。选用200只65周龄罗曼粉壳蛋鸡作为试验动物，随机分成4组，每组5个重复，每个重复10只。对照组饲喂基础饲粮（钼含量为1.50 mg/kg），其余3组分别饲喂在基础饲粮基础上以钼酸钠形式添加10（M10组）、20（M20组）、100 mg/kg钼（M100组）的试验饲粮。试验期为5周。结果表明：1）饲粮中添加钼可线性降低蛋鸡的产蛋率（P=0.04）。与对照组相比，M100组蛋鸡的产蛋率降低5.62%，料蛋比增加11.98%，破壳蛋率增加168%，差异均达到显著水平（P<0.05）。2）饲粮中添加钼可线性降低蛋壳厚度（P=0.04），线性降低蛋黄颜色（P=0.01），线性增加蛋壳亮度值（P=0.01），线性降低蛋壳红度值（P=0.04）。与对照组相比，M20和M100组的蛋壳厚度显著降低（P<0.05），M100组的蛋黄颜色显著降低（P<0.05）。3）与对照组相比，M100组血清中谷草转氨酶（AST）、谷丙转氨酶（ALT）和碱性磷酸酶（AKP）活性显著提高（P<0.05），提升率分别为46.02%、51.99%和15.72%。M100组血清中尿酸（UA）含量显著低于其他各组（P<0.05）。血清中黄嘌呤氧化酶（XOD）活性随钼添加量的升高呈现二次曲线变化（P<0.05）。与对照组相比，M10和M20组血清中XOD活性分别提高了85.93%和101.14%（P<0.05），而M100组则降低了46.64%（P<0.05）。与对照组相比，M100组肝脏总抗氧化能力（T-AOC）和谷胱甘肽过氧化物酶（GSH-Px）活性显著降低（P<0.05），还原性谷胱甘肽（GSH）和丙二醛（MDA）含量显著增加（P<0.05）。综上所述，在饲粮中添加10和20 mg/kg钼可增加产蛋后期蛋鸡血清中XOD活性，但对生产性能、蛋品质和肝脏抗氧化能力没有显著影响，而当饲粮中钼添加量达到100 mg/kg时，则会降低产蛋后期蛋鸡的生产性能、蛋品质以及血清中XOD活性和肝脏抗氧化能力。

关键词： 钼; 蛋鸡; 生产性能; 蛋品质; 抗氧化

本文引用格式

王建萍, 杨曾乔, 丁雪梅, 白世平, 曾秋凤, 张克英 . 高钼饲粮对产蛋后期蛋鸡生产性能、蛋品质、血清生化指标和肝脏抗氧化指标的影响[J]. 动物营养学报, 2019 , 31(1) : 140 -147 . DOI: 10.3969/j.issn.1006-267x.2019.01.019

Abstract

This experiment was conducted to study the effects of high molybdenum (Mo) diet on performance, egg quality, serum biochemical indexes and hepatic antioxidative indexes of laying hens during late laying period. A total of 200 pink-eggshell Lohnman laying hens (65-week-old) were randomly allotted into 4 groups with 5 replicates per group and 10 birds per replicate. Laying hens in control group were fed a basal diet (Mo content was 1.50 mg/kg), and those in other 3 groups were fed the basal diet supplemented with 10 (M10), 20 (M20), and 100 mg/kg Mo (M100) as sodium molybdate for 5 weeks. The results showed as follows:1) Mo supplementation linearly decreased laying rate (P=0.04). Compared with the control group, the laying rate in M100 group was decreased by 5.62% and the feed/egg and broken eggshell rate were increased by 11.98% and 168.59%, respectively, and the differences were significant (P<0.05). 2) Mo supplementation linearly reduced the egg thickness (P=0.04), yolk color (P=0.01) and eggshell lightness value (P=0.01), and linearly increased the eggshell redness value (P=0.04). The eggshell thickness in M20 and M100 groups was significantly decreased, and the yolk color in M100 group was significantly decreased compared with control group (P<0.05). 3) The activities of serum aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (AKP) in M100 group were significantly increased compared with control group (P<0.05), and the increase rates were 46.02%, 51.99% and 15.72%, respectively. The serum uric acid content in M100 group was significantly lower than that in other groups (P<0.05). Serum xanthine oxidase (XOD) activity showed a quadratic curve change with the Mo supplemental level increasing (P<0.05). Compared with the control group, the serum XOD activity in M10 and M20 groups was increased by 85.93% and 101.14% (P<0.05), respectively, while it was decreased by 46.64% in M100 group (P<0.05). Compared with the control group, the serum total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) activity in M100 group were significantly reduced (P<0.05), and the serum reduced glutathione (GSH) and malondialdehyde (MDA) contents were significantly increased (P<0.05). In conclusion, dietary supplemented with 10 and 20 mg/kg Mo can increase serum XOD activity without any effects on performance, egg quality and hepatic antioxidant ability of laying hens during late laying period, but when Mo supplemental level is 100 mg/kg, it can reduce the performance, egg quality, serum XOD activity and hepatic antioxidant ability of laying hens during late laying period.

Key words： molybdenum; laying hens; performance; egg quality; antioxidant ability

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